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Literature summary for 3.2.1.26 extracted from
- Invertases in oat seedlings (1980), Plant Physiol., 65, 135-140.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.02 mM, complete inhibition of invertase I, 44% inhibition of invertase II | Avena sativa |  |
| aniline | 10 mM, 45% inhibition of invertase I, no inhibition of invertase II | Avena sativa | |
| Hg2+ | 0.002 mM, 55% inhibition of invertase I, complete inhibition of invertase II | Avena sativa | |
| I2 | 0.004 mM, complete inhibition of invertase II, 9% inhibition of invertase I | Avena sativa | |
| PCMB | 0.002 mM, 21% inhibition of invertase I, 79% inhibition of invertase II | Avena sativa | |
| pyridoxal | 1 mM, 65% inhibition of invertase I, 25% inhibition of invertase II | Avena sativa | |
| pyridoxamine | 10 mM, 28% inhibition of invertase I, 65% inhibition of invertase II | Avena sativa | |
| pyridoxine | 2.5 mM, 70% inhibition of invertase I, 20% inhibition of invertase II | Avena sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
sucrose | invertase I | Avena sativa | |
| 2.9 | - |
raffinose | invertase I | Avena sativa | |
| 6.7 | - |
sucrose | invertase II | Avena sativa | |
| 14 | - |
stachyose | invertase I | Avena sativa | |
| 17 | - |
raffinose | invertase II | Avena sativa | |
| 25 | - |
stachyose | invertase II | Avena sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
invertase I, gel filtration | Avena sativa |
| 108000 | - |
invertase II, gel filtration | Avena sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | - |
2 invertases: I and II | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| coleoptile | high invertase activity in the apical segment of the coleoptile, the level of activity decreases sharply with distance from the apex | Avena sativa | - |
| leaf | low activity | Avena sativa | - |
| root | low activity | Avena sativa | - |
| seedling | highest activity at the apex of the coleoptile, much lower in the primary leaf, mesocotyl and root | Avena sativa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| refrigerated, stable for 4 weeks | Avena sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | - |
Avena sativa | fructose + melibiose | - |
? | |
| stachyose + H2O | - |
Avena sativa | ? | - |
? | |
| sucrose + H2O | - |
Avena sativa | D-fructose + D-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
at pH 5 or higher, stable for at least 30 min, invertase II | Avena sativa |
| 47 | - |
pH 4.5, 5 min, 50% loss of activity | Avena sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
invertase I | Avena sativa |
| 5 | - |
invertase II | Avena sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | pH 3.0: about 50% of maximal activity, pH 5.0: about 80% of maximal activity, invertase II | Avena sativa |
| 4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 20% of maximal activity | Avena sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
0°C, 30 min, stable, invertase I | Avena sativa |
| 3.5 | - |
low stability at or below, invertase II | Avena sativa |
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