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Literature summary for 3.2.1.26 extracted from

  • Du, L.; Pang, H.; Wang, Z.; Lu, J.; Wei, Y.; Huang, R.
    Characterization of an invertase with pH tolerance and truncation of its N-terminal to shift optimum activity toward neutral pH (2013), PLoS ONE, 8, e62306.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.8
-
sucrose at pH 4.5 and 45°C uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE uncultured bacterium
65000
-
x * 65000, MALDI-TOF mass spectrometry uncultured bacterium
66700
-
x * 66700, calculated from amino acid sequence uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium F1CGX0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography uncultured bacterium

Storage Stability

Storage Stability Organism
37°C, pH 7.0, 30 days, the enzyme remains stable uncultured bacterium
4°C, pH 4.5, 30 days, the enzyme remains stable uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
uncultured bacterium D-fructose + D-glucose + sucrose
-
?
inulin + H2O
-
uncultured bacterium D-fructose + ?
-
?
nystose + H2O
-
uncultured bacterium D-fructose + D-glucose + sucrose
-
?
raffinose + H2O
-
uncultured bacterium D-fructose + melibiose
-
?
sucrose + H2O
-
uncultured bacterium D-fructose + D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE uncultured bacterium
? x * 65000, MALDI-TOF mass spectrometry uncultured bacterium
? x * 66700, calculated from amino acid sequence uncultured bacterium

Synonyms

Synonyms Comment Organism
acid invertase
-
uncultured bacterium
uninv2
-
uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 more than 50% activity between 35 and 55°C uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1448
-
sucrose at pH 4.5 and 45°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 more than 50% activity betwenn pH 3.5 and 6.0 uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme retains 100% activity at pH 5.0 for 120 min. The activity is reduced to more than 70% after 360 min at pH 5.0-8.0 uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
81.3
-
sucrose at pH 4.5 and 45°C uncultured bacterium