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Literature summary for 3.2.1.26 extracted from
- Studies on kinetics and thermostability of a novel acid invertase from Fusarium solani (2006), J. Agric. Food Chem., 54, 4617-4623.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-chymotrypsin | 0.5 mg/ml | Fusarium solani | |
| additional information | Pb2+ and Hg2+ do not inhibit the enzyme activity at a concentration of 1 mM | Fusarium solani | |
| Urea | 4 M | Fusarium solani |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.57 | - |
sucrose | in MES buffer, pH 5.0, at 40°C | Fusarium solani | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 50% increased activity at 1 mM | Fusarium solani | |
| Co2+ | 69% increased activity at 1 mM | Fusarium solani | |
| Cu2+ | 120% increased activity at 1 mM | Fusarium solani | |
| K+ | 20% increased activity at 1 mM | Fusarium solani | |
| Mg2+ | 105% increased activity at 1 mM | Fusarium solani | |
| Na+ | 60% increased activity at 1 mM | Fusarium solani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Fusarium solani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium solani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration | Fusarium solani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.92 | - |
crude extract, at pH 5.0 and 40 ?C | Fusarium solani |
| 245.6 | - |
after 3.11fold purification, at pH 5.0 and 40 ?C | Fusarium solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | - |
Fusarium solani | D-glucose + D-fructose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid invertase | - |
Fusarium solani |
| beta-D-fructofuranosidase | - |
Fusarium solani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fusarium solani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
- |
Fusarium solani |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
an increase in pH beyond optimum causes a rapid inactivation of the enzyme with 100% loss in activity observed at pH 5.0 | Fusarium solani |
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