Cloned (Comment) | Organism |
---|---|
overexpression of a truncated version of InvA (residues Lys9-Thr460) | Nostoc sp. PCC 7120 = FACHB-418 |
Crystallization (Comment) | Organism |
---|---|
partial proteolysis and multiple sequence alignment indicate that both N and C termini are somewhat unstructured. Thus, we overexpressed and purified a truncated version of InvA (residues Lys9-Thr460) and successfully optimized its crystal co-crystallized with 200 mM fructose, the 2.11 A SeMet-substituted crystal of which was used for structure determination. In the sucrose-complexed structure (InvA-Suc), which has a space group of C2221, each asymmetric unit contains three molecules of InvA | Nostoc sp. PCC 7120 = FACHB-418 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.7 | - |
sucrose | pH and temperature not specified in the publication | Nostoc sp. PCC 7120 = FACHB-418 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Nostoc sp. PCC 7120 = FACHB-418 | the enzyme plays a key role in primary metabolism and plant development | beta-D-fructose + alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. PCC 7120 = FACHB-418 | Q8YWS9 | - |
- |
Purification (Comment) | Organism |
---|---|
truncated version of InvA (residues Lys9-Thr460) | Nostoc sp. PCC 7120 = FACHB-418 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Nostoc sp. PCC 7120 = FACHB-418 | beta-D-fructose + alpha-D-glucose | - |
? | |
sucrose + H2O | the enzyme plays a key role in primary metabolism and plant development | Nostoc sp. PCC 7120 = FACHB-418 | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | invertase InvA exists as a hexamer in both crystal and solution | Nostoc sp. PCC 7120 = FACHB-418 |
Synonyms | Comment | Organism |
---|---|---|
alkaline invertase InvA | - |
Nostoc sp. PCC 7120 = FACHB-418 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Nostoc sp. PCC 7120 = FACHB-418 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 40% of maximal activity, pH 9.0: about 60% of maximal activity | Nostoc sp. PCC 7120 = FACHB-418 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a key role in primary metabolism and plant development | Nostoc sp. PCC 7120 = FACHB-418 |