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Literature summary for 3.2.1.26 extracted from
- Production and characterization of a novel yeast extracellular invertase activity towards improved dibenzothiophene biodesulfurization (2014), Appl. Biochem. Biotechnol., 174, 2048-2057.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | about 90% residual activity at 10 mM | Zygosaccharomyces bailii |  |
| MoO42- | about 60% residual activity at 10 mM | Zygosaccharomyces bailii | |
| WO42 | about 25% residual activity at 10 mM | Zygosaccharomyces bailii | |
| Zn2+ | about 80% residual activity at 10 mM | Zygosaccharomyces bailii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Zygosaccharomyces bailii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | about 140% activity at 1 mM | Zygosaccharomyces bailii | |
| Ca2+ | about 175% activity at 1 mM | Zygosaccharomyces bailii | |
| Co2+ | about 130% activity at 1 mM | Zygosaccharomyces bailii | |
| Cu2+ | about 120% activity at 1 mM | Zygosaccharomyces bailii | |
| Fe3+ | about 110% activity at 1 mM | Zygosaccharomyces bailii | |
| Mn2+ | about 190% activity at 1 mM | Zygosaccharomyces bailii | |
| additional information | the presence of Mg2+, Zn2+, MoO42-, and WO42- causes no significant effect on invertase activity at 1 mM | Zygosaccharomyces bailii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zygosaccharomyces bailii | - |
- |
- |
| Zygosaccharomyces bailii Talf1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | - |
Zygosaccharomyces bailii | D-fructose + D-glucose | - |
? | |
| sucrose + H2O | - |
Zygosaccharomyces bailii Talf1 | D-fructose + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| invertase | - |
Zygosaccharomyces bailii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Zygosaccharomyces bailii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | high stability at 30°C (after 13 days 40% remaining activity). At 60°C, a residual invertase activity of about 14% is observed, and above 65°C, all invertase activities are inactivated due enzyme denaturation within 30 min. For 45°C, an invertase activity loss of about 45 and 70% is observed within 3 and 6 h, respectively, with the total enzyme denaturation occurring within around 24 h | Zygosaccharomyces bailii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Zygosaccharomyces bailii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | high stability at pH 5.5-6.5. The pH increase to 6.5 and 7.5 influence severely the enzyme stability, remaining about 5% invertase activity after 1 day at pH 7.5 and 7 days at pH 6.5. Thus, a sharply loss of invertase activity is observed for pH 7.5 | Zygosaccharomyces bailii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Zygosaccharomyces bailii | Jerusalem artichoke acts as the strongest inducer of enzyme activity | up |
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Release 2023.1 (January 2023)
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