Cloned (Comment) | Organism |
---|---|
gene lbbetafruct2, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris | Ipomoea batatas |
gene lbbetafruct3, DNA and amino acid sequence determination and analysis, expression of the His-tagged soluble, vacuolar isozyme in Pichia pastoris | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.97 | - |
sucrose | pH 5.0, recombinant isozyme lbbetafruct3 | Ipomoea batatas | |
10.1 | - |
sucrose | pH 5.0, recombinant isozyme lbbetafruct2 | Ipomoea batatas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | vacuolar acid invertase | Ipomoea batatas | - |
- |
vacuole | acid invertase | Ipomoea batatas | 5773 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | Q94C05 | isozyme lbbetafruct3; cv. Tainong 57 | - |
Ipomoea batatas | Q94C06 | isozyme lbbetafruct2; cv. Tainong 57 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation by PMGase F, the glycosylation of isozyme lbbetafruct2 differs in the different plant tissues and between native and recombinant enzyme, overview | Ipomoea batatas |
glycoprotein | deglycosylation by PMGase F, the glycosylation of isozyme lbbetafruct3 differs in the different plant tissues and between native and recombinant enzyme, overview | Ipomoea batatas |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged soluble, vacuolar isozyme lbbetafruct3 5fold from Pichia pastoris by nickel affinity chromatography | Ipomoea batatas |
recombinant His-tagged soluble, vacuolar isozyme lbbetalbbetafruct2 7.6fold from Pichia pastoris by nickel affinity chromatography | Ipomoea batatas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young and mature, expression of isozyme lbbetafruct3 is restricted to shoots and leaves | Ipomoea batatas | - |
additional information | isozyme lbbetafruct2 is expressed in most tissues, except for mature leaves and sprouting storage roots, exspecially in sink organs | Ipomoea batatas | - |
shoot | expression of isozyme lbbetafruct3 is restricted to shoots and leaves | Ipomoea batatas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant isozyme lbbetafruct3 | Ipomoea batatas |
553 | - |
purified recombinant isozyme lbbetafruct2 | Ipomoea batatas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct2 | Ipomoea batatas | ? | - |
? | |
additional information | activity with cellobiose, maltose, lactose, and inulin, by isozyme lbbetafruct3 | Ipomoea batatas | ? | - |
? | |
raffinose + H2O | 25% of the activity with sucrose, recombinant isozyme lbbetafruct3 | Ipomoea batatas | alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose | - |
? | |
raffinose + H2O | 37% of the activity with sucrose, recombinant isozyme lbbetafruct2 | Ipomoea batatas | alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose | - |
? | |
stachyose + H2O | 13% of the activity with sucrose, recombinant isozyme lbbetafruct2 | Ipomoea batatas | ? | - |
? | |
stachyose + H2O | 9% of the activity with sucrose, recombinant isozyme lbbetafruct3 | Ipomoea batatas | ? | - |
? | |
sucrose + H2O | preferred substrate, recombinant isozyme lbbetafruct2 | Ipomoea batatas | D-glucose + D-fructose | - |
? | |
sucrose + H2O | preferred substrate, recombinant isozyme lbbetafruct3 | Ipomoea batatas | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | analysis of conserved notifs and segments in the secondray structure of isozyme lbbetafruct2, overview | Ipomoea batatas |
More | analysis of conserved notifs and segments in the secondray structure of isozyme lbbetafruct3, overview | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
lbbetafruct2 | - |
Ipomoea batatas |
lbbetafruct3 | - |
Ipomoea batatas |
soluble acid invertase | - |
Ipomoea batatas |
Vacuolar invertase | - |
Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant isozyme lbbetafruct2 | Ipomoea batatas |
5 | - |
recombinant isozyme lbbetafruct3 | Ipomoea batatas |