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Literature summary for 3.2.1.26 extracted from

  • Kocal, N.; Sonnewald, U.; Sonnewald, S.
    Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv vesicatoria (2008), Plant Physiol., 148, 1523-1536.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
Lin8 400 bp fragment inserted into plant transformation vector pK7GWIWG2(II), transformed into tomato plants via Agrobacterium tumefaciens-mediated gene transfer Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall two isoforms: Lin6 and Lin8 Solanum lycopersicum 5618
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content ?
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 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
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 var. Moneymaker wild-type and with silenced leaf isoforms of cw-Inv, interaction with Xanthomonas campestris pv vesicatoria infection
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
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 Solanum lycopersicum
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additional information tomato lines with strongly reduced source leaf cell wall-bound invertase activity by RNAi inhibition, 10-15% source leaf activity, while sink leaves and petioles still have 40-60% of wild-type activity, no alterations in other organs and tissue: Lin8-RNAi line 33, line 50, line 57, gene-silencing Solanum lycopersicum
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 Lin8-RNAi line 33 activity: 3.4 micromol/squaremeter/min, vacuolar invertase: 3.6 micromol/squaremeter/min Solanum lycopersicum
additional information
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 Lin8-RNAi line 50 activity: 3.6 micromol/squaremeter/min, vacuolar invertase: 5.6 micromol/squaremeter/min Solanum lycopersicum
additional information
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 Lin8-RNAi line 57 activity: 1.5 micromol/squaremeter/min, vacuolar invertase: 4.0 micromol/squaremeter/min Solanum lycopersicum
additional information
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 wild-type activity: 40.5 micromol/squaremeter/min, vacuolar invertase: 4.2 micromol/squaremeter/min Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content Solanum lycopersicum ?
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 ?
sucrose + H2O
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 Solanum lycopersicum D-fructose + D-glucose
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 ?

Synonyms

Synonyms Comment Organism
cell wall-bound invertase
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 Solanum lycopersicum