Information on EC 3.1.3.8 - 3-phytase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.3.8
-
RECOMMENDED NAME
GeneOntology No.
3-phytase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
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PATHWAY
KEGG Link
MetaCyc Link
Inositol phosphate metabolism
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phytate degradation I
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SYSTEMATIC NAME
IUBMB Comments
myo-inositol-hexakisphosphate 3-phosphohydrolase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1-phytase
-
-
-
-
168phyA
-
-
168phyA
-
-
-
3'-phytase
-
-
3-phytase
-
-
3-phytase
Schwanniomyces capriottii CBS 2923
-
-
-
3-phytase A
P34752
-
acid phytase
-
-
acid phytase
Aspergillus niger FS3
-
-
-
acidic histidine acid phosphatase
G4WZU3
-
acidic histidine acid phosphatase
Serratia sp. TN49, ACCC 03909
G4WZU3
-
-
alkaline beta-propeller phytase
Janthinobacterium sp.
G0XZM1
-
alkaline beta-propeller phytase
Janthinobacterium sp. TN115, ACCC 5613
G0XZM1
-
-
alkaline beta-propeller phytase
G4WZU4
-
alkaline beta-propeller phytase
Serratia sp. TN49, ACCC 03909
G4WZU4
-
-
alkaline phytase
-
-
alkaline phytase
-
-
-
alkaline phytase
Q0GYS1, Q0GYS2
-
Allzyme
-
commercial preparation
Allzyme phytase
-
commercial preparation
AppA
-
gene name
AppA
D9D7K9
gene name
AppA
Q7CIZ7
-
appA phytase
-
-
AppA2
Q6RK08
gene name
AppA2 phytase
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
ASR1
Q84CN9
-
beta-propeller phytase
-
beta-propeller phytase has substrate specificity for myo-inositol hexakisphosphate and yields a myo-inositol trisphosphate and three phosphate groups as the final products
beta-propeller phytase
Bacillus amyloliquefaciens DS11
-
beta-propeller phytase has substrate specificity for myo-inositol hexakisphosphate and yields a myo-inositol trisphosphate and three phosphate groups as the final products
-
beta-propeller phytase
-
-
beta-propeller phytase
-
-
-
beta-propeller phytase
O31097, P42094, Q70E78, Q84B22, Q93GB6, Q9F657
-
beta-propeller phytase
-
-
beta-propeller phytase
G4WZU4
-
beta-propeller phytase
Serratia sp. TN49, ACCC 03909
G4WZU4
-
-
BPP
-
-
-
cell-bound phytase
D3HIF3
-
cysteine phytase
-
-
dual-domain BPP
-
-
dual-domain BPP
-
-
-
experimental phytase SP 1002
-
-
HAP
Q84CN9
-
HAP phytase
D9D7K9
-
histidine acid phosphatase
-
-
histidine acid phosphatase
Q6RK08
-
histidine acid phosphatase
Q84CN9
-
histidine acid phosphatase
D3HIF3
-
histidine acid phytase
G4WZU3
-
histidine acid phytase
Serratia sp. TN49, ACCC 03909
G4WZU3
-
-
LlALP1
Q0GYS1
-
LlALP2
Q0GYS2
-
microbial phytase
-
-
MOK1 phytase
-
-
myo-inositol hexakiphosphate phosphohydrolase
O00092
-
myo-inositol hexakiphosphate phosphohydrolase
Aspergillus fumigatus WY-2
O00092
-
-
myo-inositol hexakis phosphohydrolase
-
-
myo-inositol hexakisphosphate 3-phosphohydrolase
-
-
myo-inositol hexakisphosphate 3-phosphohydrolase
Schwanniomyces capriottii CBS 2923
-
-
-
myo-inositol hexaphosphate phosphohydrolase
Q6T9Z6
-
myo-inositol hexaphosphate phosphohydrolase
Aspergillus niger N-J
Q6T9Z6
-
-
myo-inositol hexaphosphate phosphohydrolase
-
-
myo-inositol hexaphosphate phosphohydrolase
Myceliophthora heterothallica BJTLR50
-
-
-
MYO-inositol-hexaphosphate 3-phosphohydrolase
-
-
-
-
myo-inositol-hexaphosphate phosphohydrolase
-
-
Natuphos
-
commercial preparation
Natuphos
-
commercial preparation
neutral phytase
A4UU76
-
neutral phytase
Bacillus licheniformis ZJ-6
A4UU76
-
-
PAP
C4PKL2, C4PKL4
-
PAP
C4PKK7, C4PKK9
-
PAP
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
PAP
C4PKL6
-
PAP type I
C4PKL2, C4PKL4
-
PAP type I
C4PKK7, C4PKK9
-
PAP type I
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
PAP type I
C4PKL6
-
PAPhy
C4PKK7, C4PKK8
-
PAPhys
C4PKL2, C4PKL4
-
PAPhys
C4PKK7, C4PKK9
-
PAPhys
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
PAPhys
C4PKL6
-
pGF11 phytase
-
recombinant enzyme
pGP209 phytase
-
recombinant enzyme
pH 2.5 optimum acid phosphatase
-
-
-
-
PhyA
Aspergillus japonicus BCC18313
B3VPB2
-
-
PhyA
-
gene name
PhyA
B3VPB3
-
PhyA
P34752
-
PhyA
Q6T9Z6
-
PhyA
Aspergillus niger BCC18081
B3VPB3
-
-
PhyA
Aspergillus niger N-J
Q6T9Z6
-
-
PhyA
Aspergillus niger NRRL 3135
P34752
-
-
PhyA
Kodamaea ohmeri BG3
-
-
-
PhyA
Myceliophthora heterothallica BJTLR50
-
-
-
PhyA
Schwanniomyces capriottii CBS 2923
-
-
-
PhyA phytase
P34752
-
PhyA115
Janthinobacterium sp.
G0XZM1
gene name
PhyA115
Janthinobacterium sp. TN115, ACCC 5613
G0XZM1
gene name
-
PhyB49
G4WZU4
-
PhyB49
Serratia sp. TN49, ACCC 03909
G4WZU4
-
-
PhyC
O31097
-
PhyC
Bacillus subtilis VTT E-68013
-, O31097
-
-
PhyCm
Bacillus licheniformis ZJ-6
A4UU76
-
-
PhyH
-
-
PhyH
-
-
-
PhyH49
G4WZU3
-
PhyH49
Serratia sp. TN49, ACCC 03909
G4WZU3
-
-
PhyK
Q84CN9
-
PhyK
Klebsiella sp. ASR1
-
-
-
PhyP
-
-
phytase
-
-
-
-
phytase A
-
-
phytase C
O31097
-
phytase C
P42094, Q70E78, Q84B22, Q93GB6, Q9F657
-
phytase C
Bacillus subtilis VTT E-68013
O31097
-
-
phytate 1-phosphatase
-
-
-
-
Phytate 3-phosphatase
-
-
-
-
phytate 6-phosphatase
-
-
-
-
PPHY
D3HIF3
gene name
protease-resistant phytase
A2TEY6
-
purple acid phosphatase
C4PKL2, C4PKL4
-
purple acid phosphatase
C4PKK7
-
purple acid phosphatase
C4PKK8
-
purple acid phosphatase
C4PKK9
-
purple acid phosphatase
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
purple acid phosphatase
C4PKL6
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Ronozyme P
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commercial preparation
MOK1 phytase
Pseudomonas syringae MOK1.
-
-
-
additional information
Q84CN9
phytase ASR1 is a member of the histidine-acid-phosphatase family, that shares two conserved active-site motifs, RHGXRXP and HD, and hydrolyzes metal-free phytate with pH optima in the acidic range
additional information
D3HIF3
PPHY is a phytase belonging to histidine acid phosphatases
CAS REGISTRY NUMBER
COMMENTARY
37288-11-2
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
purple acid phosphatase with phytase activity
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-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. Growth and phosphorus nutrition of the plants supplied with phytate is improved significantly when the phytase gene from Aspergillus niger is introduced. The Aspergillus phytase is only effective when secreted as an extracellular enzyme by inclusion of the signal peptide sequence from the carrot extensin gene
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-
Manually annotated by BRENDA team
gene phyA
UniProt
Manually annotated by BRENDA team
Aspergillus aculeatus RCEF 4894
gene phyA
UniProt
Manually annotated by BRENDA team
ATCC 11382 and ATCC 11358; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
var. awamorii ATCC 38854. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
NRRL 4875 and PCC 104; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
; strain NTG-23
-
-
Manually annotated by BRENDA team
strain K; strain NRRL 3135; strain WB 320; strain WB 364; strain WB 4016; strain WB 4541; strain WB4781; strain X
-
-
Manually annotated by BRENDA team
strain NRRL 3135
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus ficuum K
strain K
-
-
Manually annotated by BRENDA team
Aspergillus ficuum NRRL 3135
strain NRRL 3135
-
-
Manually annotated by BRENDA team
Aspergillus ficuum NTG-23
; strain NTG-23
-
-
Manually annotated by BRENDA team
Aspergillus ficuum WB 320
strain WB 320
-
-
Manually annotated by BRENDA team
Aspergillus ficuum WB 364
strain WB 364
-
-
Manually annotated by BRENDA team
Aspergillus ficuum WB 4016
strain WB 4016
-
-
Manually annotated by BRENDA team
Aspergillus ficuum WB 4541
strain WB 4541
-
-
Manually annotated by BRENDA team
Aspergillus ficuum WB4781
strain WB4781
-
-
Manually annotated by BRENDA team
Aspergillus ficuum X
strain X
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
3-phytase A precursor; strain WY-2
SwissProt
Manually annotated by BRENDA team
; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
ATCC 130703. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus fumigatus WY-2
3-phytase A precursor; strain WY-2
SwissProt
Manually annotated by BRENDA team
strain BCC18313
UniProt
Manually annotated by BRENDA team
Aspergillus japonicus BCC18313
strain BCC18313
UniProt
Manually annotated by BRENDA team
3-phytase A precursor
SwissPRot
Manually annotated by BRENDA team
; The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-
-
Manually annotated by BRENDA team
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
gene phyA
SwissPRot
Manually annotated by BRENDA team
gene phyI1s
-
-
Manually annotated by BRENDA team
isolated from soil samples collected in Northeast Parana State in Brazil
-
-
Manually annotated by BRENDA team
japonicus saito ATCC 1034; NRRL 326; NRRL 330; NRRL337; NRRL372; NRRL 372; NRRL 4361; strain ATCC 10864; strain ATCC 9142; strain van Tieghem; var. cinnamoneum NRRL 348
-
-
Manually annotated by BRENDA team
NRRL 3135
-
-
Manually annotated by BRENDA team
NRRL 3135. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
recombinant enzyme from strain van Tieghem; strain NRRL 3135
-
-
Manually annotated by BRENDA team
strain 92 and strain NRRL 3135. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissPRot
Manually annotated by BRENDA team
strain 92; strain IIIAn/8; strain NRRL 3135
-
-
Manually annotated by BRENDA team
strain BCC18081
UniProt
Manually annotated by BRENDA team
strain CB; strain Naturphos
SwissPRot
Manually annotated by BRENDA team
strain N-J
UniProt
Manually annotated by BRENDA team
strain NRRL 65
-
-
Manually annotated by BRENDA team
strain NRRL3135. The T213 phytase differs from NRRL 3135 phytase at 12 amino acid residues.S13 in NRRL 3135 and A14 in T213, S30 in NRRL 3135 and T14 in T213, E66 in NRRL 3135 and D66 in T213, D89 in NRRL 3135 and E89 in T213, A106 in NRRL 3135 and V106 in T213, V155 in NRRL 3135 and I155 in T213, K171 in NRRL 3135 and E171 in T213, V236 in NRRL 3135 and A236 in T213, N292 in NRRL 3135 and H292 in T213, Q297 in NRRL 3135 and R297 in T213, S345 in NRRL 3135 and N345 in T213, V438 in NRRL 3135 and I438 in T213. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.; strain T213. The T213 phytase differs from NRRL 3135 phytase at 12 amino acid residues.S13 in NRRL 3135 and A14 in T213, S30 in NRRL 3135 and T14 in T213, E66 in NRRL 3135 and D66 in T213, D89 in NRRL 3135 and E89 in T213, A106 in NRRL 3135 and V106 in T213, V155 in NRRL 3135 and I155 in T213, K171 in NRRL 3135 and E171 in T213, V236 in NRRL 3135 and A236 in T213, N292 in NRRL 3135 and H292 in T213, Q297 in NRRL 3135 and R297 in T213, S345 in NRRL 3135 and N345 in T213, V438 in NRRL 3135 and I438 in T213. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain SK57. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
strain T213
-
-
Manually annotated by BRENDA team
strain van Teighem
-
-
Manually annotated by BRENDA team
The enzyme exhibits phytase and acid phosphatase activity. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. Growth and phosphorus nutrition of the plants supplied with phytate is improved significantly when the phytase gene from Aspergillus niger is introduced.The Aspergillus phytase is only effective when secreted as an extracellular enzyme by inclusion of the signal peptide sequence from the carrot extensin gene
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-
-
Manually annotated by BRENDA team
van Teighem
-
-
Manually annotated by BRENDA team
van Teighem. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
variant ficuum
-
-
Manually annotated by BRENDA team
Aspergillus niger 113
gene phyI1s
-
-
Manually annotated by BRENDA team
Aspergillus niger 92
strain 92
-
-
Manually annotated by BRENDA team
Aspergillus niger BCC18081
strain BCC18081
UniProt
Manually annotated by BRENDA team
Aspergillus niger CB
strain CB
SwissPRot
Manually annotated by BRENDA team
Aspergillus niger FS3
isolated from soil samples collected in Northeast Parana State in Brazil
-
-
Manually annotated by BRENDA team
Aspergillus niger IIIAn/8
strain IIIAn/8
-
-
Manually annotated by BRENDA team
Aspergillus niger N-J
strain N-J
UniProt
Manually annotated by BRENDA team
Aspergillus niger Naturphos
strain Naturphos
SwissPRot
Manually annotated by BRENDA team
Aspergillus niger NRRL 3135
gene phyA
SwissPRot
Manually annotated by BRENDA team
Aspergillus niger NRRL 3135
NRRL 3135
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 3135
strain NRRL 3135
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 326
NRRL 326
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 330
NRRL 330
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 372
NRRL 372
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 4361
NRRL 4361
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL 65
strain NRRL 65
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL3135.
strain NRRL3135. The T213 phytase differs from NRRL 3135 phytase at 12 amino acid residues.S13 in NRRL 3135 and A14 in T213, S30 in NRRL 3135 and T14 in T213, E66 in NRRL 3135 and D66 in T213, D89 in NRRL 3135 and E89 in T213, A106 in NRRL 3135 and V106 in T213, V155 in NRRL 3135 and I155 in T213, K171 in NRRL 3135 and E171 in T213, V236 in NRRL 3135 and A236 in T213, N292 in NRRL 3135 and H292 in T213, Q297 in NRRL 3135 and R297 in T213, S345 in NRRL 3135 and N345 in T213, V438 in NRRL 3135 and I438 in T213. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL337
NRRL337
-
-
Manually annotated by BRENDA team
Aspergillus niger NRRL372
NRRL372
-
-
Manually annotated by BRENDA team
Aspergillus niger SK57.
strain SK57. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
Aspergillus niger T213
strain T213
-
-
Manually annotated by BRENDA team
Aspergillus niger T213.
strain T213. The T213 phytase differs from NRRL 3135 phytase at 12 amino acid residues.S13 in NRRL 3135 and A14 in T213, S30 in NRRL 3135 and T14 in T213, E66 in NRRL 3135 and D66 in T213, D89 in NRRL 3135 and E89 in T213, A106 in NRRL 3135 and V106 in T213, V155 in NRRL 3135 and I155 in T213, K171 in NRRL 3135 and E171 in T213, V236 in NRRL 3135 and A236 in T213, N292 in NRRL 3135 and H292 in T213, Q297 in NRRL 3135 and R297 in T213, S345 in NRRL 3135 and N345 in T213, V438 in NRRL 3135 and I438 in T213. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
ATCC 11362; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus syndowi
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain 9A-1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain 9A1; strain CBS
-
-
Manually annotated by BRENDA team
strain CBS. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
Aspergillus terreus 9A-1
strain 9A-1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus terreus 9A1
strain 9A1
-
-
Manually annotated by BRENDA team
Aspergillus terreus CBS
strain CBS
-
-
Manually annotated by BRENDA team
Aspergillus terreus CBS
strain CBS. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
NRRL 4875; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Aspergillus tubingensis NRRL 4875
NRRL 4875
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain S11. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Bacillus amyloliquefaciens DS11
strain DS11
-
-
Manually annotated by BRENDA team
Bacillus amyloliquefaciens DSM7
strain DSM7
-
-
Manually annotated by BRENDA team
Bacillus amyloliquefaciens S11.
strain S11. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-
-
Manually annotated by BRENDA team
Bacillus licheniformis ZJ-6
gene phyC
UniProt
Manually annotated by BRENDA team
gene phyH
-
-
Manually annotated by BRENDA team
strain DECSR1
UniProt
Manually annotated by BRENDA team
strain DS1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain KHU-10. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain DECSR1
UniProt
Manually annotated by BRENDA team
Bacillus sp. DS1.
strain DS1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
gene phyH
-
-
Manually annotated by BRENDA team
Bacillus sp. KHU-10.
strain KHU-10. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
3-phytase
UniProt
Manually annotated by BRENDA team
isozymes FTE, FTEII, and FBA
UniProt
Manually annotated by BRENDA team
KCCM 90097
-
-
Manually annotated by BRENDA team
strain US417
UniProt
Manually annotated by BRENDA team
strain VTT E-68013
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
var. natto
-
-
Manually annotated by BRENDA team
Bacillus subtilis CF92
KCCM 90097
-
-
Manually annotated by BRENDA team
Bacillus subtilis US417
strain US417
UniProt
Manually annotated by BRENDA team
Bacillus subtilis VTT E-68013
3-phytase
UniProt
Manually annotated by BRENDA team
Bacillus subtilis VTT E-68013
strain VTT E-68013
-
-
Manually annotated by BRENDA team
Bifidobacterium adolescentis ATCC15703
ATCC15703
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
genes phyG1 and phyG51
-
-
Manually annotated by BRENDA team
strain YH-15. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Citrobacter braakii YH-15
strain YH-15. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain ABO 510
-
-
Manually annotated by BRENDA team
strain AL27
-
-
Manually annotated by BRENDA team
Cryptococcus laurentii ABO 510
strain ABO 510
-
-
Manually annotated by BRENDA team
Cryptococcus laurentii AL27
strain AL27
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
a low molecular weight form and a high molecular weight form; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
isolated from rabbit ceca
-
-
Manually annotated by BRENDA team
bifunctional enzyme with acid phosphatase and phytase activities
-
-
Manually annotated by BRENDA team
gene APPA
-
-
Manually annotated by BRENDA team
gene AppA2
UniProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Eupenicillium parvum
strain BCC1769
-
-
Manually annotated by BRENDA team
Eupenicillium parvum BCC1769
strain BCC1769
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
L. Merr.. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
isozyme a; Golden Promise, isozyme a
UniProt
Manually annotated by BRENDA team
isozyme b2; Golden Promise, isozyme b2
UniProt
Manually annotated by BRENDA team
ssp. spontaneum, ssp. agriocrithum, and ssp. vulgare
-
-
Manually annotated by BRENDA team
Janthinobacterium sp.
symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae, gene phyA115
UniProt
Manually annotated by BRENDA team
Janthinobacterium sp. TN115, ACCC 5613
symbiotic bacterial strain isolated from the gut contents of Batocera horsfieldi larvae, gene phyA115
UniProt
Manually annotated by BRENDA team
MO-3; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain MO-3; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strainMO-3. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Klebsiella oxytoca MO-3
MO-3
-
-
Manually annotated by BRENDA team
Klebsiella oxytoca MO-3
strain MO-3
-
-
Manually annotated by BRENDA team
No. PG-2; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain ASR1
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Klebsiella sp. ASR1
ASR1
-
-
Manually annotated by BRENDA team
Klebsiella sp. ASR1
strain ASR1
-
-
Manually annotated by BRENDA team
Klebsiella sp. PG-2
No. PG-2
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
fragment; strain BG3
UniProt
Manually annotated by BRENDA team
strain BG3
-
-
Manually annotated by BRENDA team
Kodamaea ohmeri BG3
fragment; strain BG3
UniProt
Manually annotated by BRENDA team
Kodamaea ohmeri BG3
strain BG3
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
LIALP1
SwissProt
Manually annotated by BRENDA team
var. amiga. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
tassi; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain 46/5(2). The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Mitsuokella multacida 46/5(2).
strain 46/5(2). The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Mucor sp.
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
; strain BJTLR50
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SWissProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Myceliophthora heterothallica BJTLR50
strain BJTLR50
-
-
Manually annotated by BRENDA team
Neurospora sp.
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
var. carotovota ACCC 10276
-
-
Manually annotated by BRENDA team
strain DSMZ 18074
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Penicillium caseoicolum
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
gene phyA; protease-resistance phytase gene phyA
UniProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain MOK1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Pseudomonas syringae MOK1.
strain MOK1. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
ATCC 22959. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
i.e. Rhizopus microsporus var. oligosporus, isozymes RO1 and RO2
-
-
Manually annotated by BRENDA team
NRRL 2710; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Rhizopus microsporus var. oligosporus NRRL 2710
NRRL 2710
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain BY85. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae BY85.
strain BY85. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae CY
strain CY
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain CBS 2923
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Schwanniomyces capriottii CBS 2923
strain CBS 2923
-
-
Manually annotated by BRENDA team
Schwanniomyces capriottii CBS 2923
strain CBS 2923
UniProt
Manually annotated by BRENDA team
Schwanniomyces castellii
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
strain JY35. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
strain JY35; The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Selenomonas ruminantium JY35
strain JY35
-
-
Manually annotated by BRENDA team
Selenomonas ruminantium JY35.
strain JY35. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
UniProt
Manually annotated by BRENDA team
alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
UniProt
Manually annotated by BRENDA team
Serratia sp. TN49, ACCC 03909
acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
UniProt
Manually annotated by BRENDA team
Serratia sp. TN49, ACCC 03909
alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
UniProt
Manually annotated by BRENDA team
Talaromyces thermophilus
-
SwissProt
Manually annotated by BRENDA team
Talaromyces thermophilus
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
SwissProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
Torulopsis candida
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
isozyme a1
UniProt
Manually annotated by BRENDA team
isozyme a1; isozyme a1
UniProt
Manually annotated by BRENDA team
isozyme a2
UniProt
Manually annotated by BRENDA team
isozyme b1; isozyme b1
UniProt
Manually annotated by BRENDA team
Triticum aestivum Bob white SH 98 26
isozyme a1; isozyme a1
UniProt
Manually annotated by BRENDA team
Triticum aestivum Bob white SH 98 26
isozyme b1; isozyme b1
UniProt
Manually annotated by BRENDA team
isolated from dried flower buds of Woodfordia fruticosa
UniProt
Manually annotated by BRENDA team
single copy gene PPHY
UniProt
Manually annotated by BRENDA team
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
-
Manually annotated by BRENDA team
gene YeappA
UniProt
Manually annotated by BRENDA team
isozyme b; isozyme b
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
G4WZU3, G4WZU4
the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most; the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most
evolution
-
dual-domain beta-propeller phytases have succeeded evolutionarily because they can increase the amount of available phosphate by interacting together
evolution
-
dual-domain beta-propeller phytases have succeeded evolutionarily because they can increase the amount of available phosphate by interacting together
-
evolution
Serratia sp. TN49, ACCC 03909
-
the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most; the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most
-
physiological function
-
gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate
physiological function
-
phytate is an antinutritional factor that influences the bioavailability of essential minerals by forming complexes with them and converting them into insoluble salts. beta-Propeller phytase hydrolyzes insoluble Ca2+-phytate salts and completely abrogates the ability of phytate to chelate metal ions, stoichiometry, affinity, and thermodynamics of these interactions by isothermal titration calorimetry, overview. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+
physiological function
-
cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment
physiological function
-
importance of enzyme domain PhyH-DI in phytate degradation
physiological function
C4PKK7, C4PKK8
phytase activity in grain is essential to make phosphate available to cell metabolism, and in food and feed; phytase activity in grain is essential to make phosphate available to cell metabolism, and in food and feed
physiological function
-
importance of enzyme domain PhyH-DI in phytate degradation
-
metabolism
-
release of phosphate by stepwise hydrolysis of phosphomonoester bonds in phytate. Phytate is the major storage form of phosphate and inositol, predominantly occurring in cereal grains, legumes, and oilseeds
additional information
-
the enzyme shows strong resistance toward pepsin and trypsin
additional information
O31097
the enzyme is resistant to shrimp digestive enzymes and porcine trypsin
additional information
D9D7K9
catalytic efficiency of the HAP phytase is determined by key residue Arg79 located in close proximity to the active site
additional information
-
conformational dynamics of the active site loop, structure analysis, overview. Molecular dynamic studies indicate that the movement in the active site is mainly confined by the active site loop resulted in wider opening of the loop in absence of phytate, possible role of loop residues as prerequisite for highly active phytases
additional information
G4WZU3, G4WZU4
two types of phytases in Serratia sp. TN49; two types of phytases in Serratia sp. TN49
additional information
Q84CN9
phytase ASR1 is a member of the histidine-acid-phosphatase family, that shares two conserved active-site motifs, RHGXRXP and HD, and hydrolyzes metal-free phytate with pH optima in the acidic range. Histidine-acid-phosphatases share a common two-step mechanism for catalysis. The reaction starts with a nucleophilic attack on the phosphoester bond by a conserved histidine in the long active-site motif. The histidine side chain from the conserved HD motif protonates the leaving group. The second step comprises hydrolysis of the resulting covalent phospho-histidine intermediate. Comparison of substrate binding of Klebsiella sp. ASR1 PhyK with Escherichia coli AppA phytase
additional information
-
the tandemly repeated domains of a beta-propeller phytase act synergistically to increase catalytic efficiency. The intact domain is responsible for phytate hydrolysis. Enzyme domain PhyH-DI also hydrolyzes the phytate intermediate D-Ins(1,4,5,6)P4, and acts synergistically, causing a 1.2-2.5fold increase in phosphate release, with domain PhyH-DII, other beta-propeller phytases, PhyP and 168PhyA, and a histidine acid phosphatase. Fusion of PhyH-DI with PhyP or 168PhyA significantly enhanced their catalytic efficiencies
additional information
-
the enzyme can also act as a vanadate haloperoxidase, EC 1.11.1.10
additional information
A2TEY6, -
replacements of G56E, L65F, Q144H, and L151S improve the thermal stability of the protein by increasing new hydrogen bonds among the adjacent secondary structures
additional information
Q0GYS1, Q0GYS2
the enzyme is a histidine phosphatase, it contains the signature heptapeptide of histidine phosphatases, -RHGXRXP- , near the N-terminus. Isozymes LlALP1 and LlALP2 possess unique catalytic properties. Substrate specificity and temperature dependence of catalysis of the recombinant isoyzme LlALP2 as well as the effect of pH, inhibitors, calcium ions, and EDTA are very similar to that of the wild-type enzyme from lily pollen; the enzyme is a histidine phosphatase, it contains the signature heptapeptide of histidine phosphatases, -RHGXRXP-, near the N-terminus. Isozymes LlALP1 and LlALP2 possess unique catalytic properties. Substrate specificity and temperature dependence of catalysis of the recombinant isoyzme LlALP2 as well as the effect of pH, inhibitors, calcium ions, and EDTA are very similar to that of the wild-type enzyme from lily pollen
additional information
-
the enzyme adsorbs to and interacts with pure minerals and oxisol clays also releasing orthophosphate from InsP6-saturated minerals, overview
additional information
Aspergillus ficuum NTG-23
-
the enzyme shows strong resistance toward pepsin and trypsin
-
additional information
-
the tandemly repeated domains of a beta-propeller phytase act synergistically to increase catalytic efficiency. The intact domain is responsible for phytate hydrolysis. Enzyme domain PhyH-DI also hydrolyzes the phytate intermediate D-Ins(1,4,5,6)P4, and acts synergistically, causing a 1.2-2.5fold increase in phosphate release, with domain PhyH-DII, other beta-propeller phytases, PhyP and 168PhyA, and a histidine acid phosphatase. Fusion of PhyH-DI with PhyP or 168PhyA significantly enhanced their catalytic efficiencies
-
additional information
Bacillus subtilis VTT E-68013
-
the enzyme is resistant to shrimp digestive enzymes and porcine trypsin
-
additional information
Serratia sp. TN49, ACCC 03909
-
two types of phytases in Serratia sp. TN49; two types of phytases in Serratia sp. TN49
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-L-myo-inositol 1,2,3,4,5-pentakisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
1-L-myo-inositol 1,2,3,5,6-pentakisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
130% of the activity with myo-inositol hexakisphosphate
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
2.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
74% of the activity with myo-inositol hexakisphosphate
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
89.6% of the activity with myo-inositol hexakisphosphate
-
?
2-glycerophosphate + H2O
glycerate + phosphate
show the reaction diagram
-
-
-
?
2-glycerophosphate + H2O
glycerate + phosphate
show the reaction diagram
-
19.8% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
2-glycerophosphate + H2O
glycerate + phosphate
show the reaction diagram
-
127% of the activity with myo-inositol hexakisphosphate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
4.9% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
63.6% of the activity with myo-inositol hexakisphosphate
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
97.1% of the activity with myo-inositol hexakisphosphate
-
?
2-phosphoglycerate + H2O
glyceric acid + phosphate
show the reaction diagram
-
-
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
-
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
-
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
-
168% relative activity compared to myo-inositol hexakisphosphate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
-
95% activity compared to myo-inositol hexakisphosphate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
-
69% of the activity with 4-nitrophenylphosphate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
show the reaction diagram
Schwanniomyces capriottii CBS 2923
-
95% activity compared to myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Q0GYS1, Q0GYS2
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Eupenicillium parvum
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
7.6% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
101% of the activity with myo-inositol hexakisphosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
11% of the activity with myo-inositol hexakisphosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
23.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
39.8% of the activity with myo-inositol hexakisphosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
59.6% of the activity with myo-inositol hexakisphosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
reaction is nearly as efficient as on phytate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
0.65% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Aspergillus ficuum, Aspergillus ficuum NTG-23
-
254% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Aspergillus terreus 9A1
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Klebsiella sp. PG-2
-
7.6% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Eupenicillium parvum BCC1769
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Aspergillus niger FS3
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
Aspergillus ficuum NRRL 3135
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenylphosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenylphosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenylphosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
5'-ATP + H2O
?
show the reaction diagram
-
250% of the activity with myo-inositol hexakisphosphate
-
?
6-phosphogluconate + H2O
gluconate + phosphate
show the reaction diagram
-
-
-
-
?
ADP + H2O
?
show the reaction diagram
-
-
-
-
?
ADP + H2O
?
show the reaction diagram
-
-
-
?
ADP + H2O
?
show the reaction diagram
-
-
-
-
?
ADP + H2O
?
show the reaction diagram
-
75% of the activity with myo-inositol hexakisphosphate
-
-
?
ADP + H2O
?
show the reaction diagram
-
14.2% of the activity with myo-inositol hexakisphosphate
-
?
ADP + H2O
?
show the reaction diagram
-
25.6% of the activity with myo-inositol hexakisphosphate
-
?
ADP + H2O
?
show the reaction diagram
-
7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
ADP + H2O
?
show the reaction diagram
Bacillus subtilis VTT E-68013
-
75% of the activity with myo-inositol hexakisphosphate
-
-
?
ADP + H2O
?
show the reaction diagram
Klebsiella sp. ASR1
-
7.5% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
-
-
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
-
120% activity compared to myo-inositol hexakisphosphate
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
-
206% relative activity compared to myo-inositol hexakisphosphate
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
Aspergillus niger FS3
-
-
-
-
?
ADP + H2O
? + phosphate
show the reaction diagram
-
0.02% of the activity with myo-inositol hexakisphosphate
-
-
?
ADP + H2O
? + phosphate
show the reaction diagram
-
177% of the activity with myo-inositol hexakisphosphate
-
-
?
ADP + H2O
? + phosphate
show the reaction diagram
-
50% of the activity with 4-nitrophenylphosphate
-
-
?
ADP + H2O
? + phosphate
show the reaction diagram
Q84B22
80% of the activity with myo-inositol hexakisphosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
-
78% activity compared to myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
no activity
-
-
-
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
25.0% of the activity with myo-inositol hexakisphosphate
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
9.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
30% relative activity compared to myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
50% activity compared to myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
Q84B22
10% of the activity with myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
173% of the activity with myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
Schwanniomyces capriottii CBS 2923
-
50% activity compared to myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
Aspergillus ficuum NTG-23
-
173% of the activity with myo-inositol hexakisphosphate
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
Klebsiella oxytoca MO-3
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
AMP + phosphate
adenosine + phosphate
show the reaction diagram
-
43.9% of the activity with myo-inositol hexakisphosphate
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
?
show the reaction diagram
-
50% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
?
show the reaction diagram
-
22.3% of the activity with myo-inositol hexakisphosphate
-
?
ATP + H2O
?
show the reaction diagram
-
38.8% of the activity with myo-inositol hexakisphosphate
-
?
ATP + H2O
?
show the reaction diagram
-
5.6% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
ATP + H2O
?
show the reaction diagram
-
reaction is nearly as efficient as on phytate
-
?
ATP + H2O
?
show the reaction diagram
Bacillus subtilis VTT E-68013
-
50% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
?
show the reaction diagram
Klebsiella oxytoca MO-3
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
133% activity compared to myo-inositol hexakisphosphate
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
-
365% relative activity compared to myo-inositol hexakisphosphate
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
Cryptococcus laurentii, Cryptococcus laurentii ABO 510
-
76.67% activity compared to myo-inositol hexakisphosphate
-
-
?
ATP + H2O
ADP + phosphate
show the reaction diagram
Aspergillus niger FS3
-
-
-
-
?
ATP + H2O
? + phosphate
show the reaction diagram
-
0.26% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
? + phosphate
show the reaction diagram
-
256% of the activity with myo-inositol hexakisphosphate
-
-
?
ATP + H2O
? + phosphate
show the reaction diagram
Q84B22
97% of the activity with myo-inositol hexakisphosphate
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
?
CTP + H2O
CDP + phosphate
show the reaction diagram
-
352% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-fructose 1,6-diphosphate + H2O
?
show the reaction diagram
-
-
-
?
D-fructose 1,6-diphosphate + H2O
?
show the reaction diagram
-
146% of the activity with myo-inositol hexakisphosphate
-
?
D-fructose 1,6-diphosphate + H2O
?
show the reaction diagram
Cryptococcus laurentii, Cryptococcus laurentii ABO 510
-
202.19% activity compared to myo-inositol hexakisphosphate
-
-
?
D-fructose 1-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 1-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
Q6U677
poor substrate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
35% activity compared to myo-inositol hexakisphosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
75% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
228% of the activity with myo-inositol hexakisphosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
Schwanniomyces capriottii CBS 2923
-
35% activity compared to myo-inositol hexakisphosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
Aspergillus ficuum NTG-23
-
228% of the activity with myo-inositol hexakisphosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
Klebsiella sp. ASR1
-
8.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
D-fructose 6-phosphate + H2O
fructose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q6U677
poor substrate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
28.6% of the activity with myo-inositol hexakisphosphate
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
92.0% of the activity with myo-inositol hexakisphosphate
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
5% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
76.72% activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 3-phosphate + H2O
glucose + phosphate
show the reaction diagram
-
75% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q6U677
poor substrate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
78.5% of the activity with myo-inositol hexakisphosphate
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
57% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
73.75% activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
82% activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
0.39% of the activity with myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
190% of the activity with myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
20% of the activity with 4-nitrophenylphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Cryptococcus laurentii ABO 510
-
73.75% activity compared to myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Aspergillus ficuum NTG-23
-
190% of the activity with myo-inositol hexakisphosphate
-
-
?
D-glucose 6-phosphate + H2O
glucose + phosphate
show the reaction diagram
-
reaction is nearly as efficient as on phytate
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
Q6U677
poor substrate
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
50% of the activity with myo-inositol hexakisphosphate
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
75% relative activity compared to myo-inositol hexakisphosphate
-
-
?
D-ribose 5-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
dATP + H2O
dADP + phosphate
show the reaction diagram
-
338% relative activity compared to myo-inositol hexakisphosphate
-
-
?
dATP + H2O
? + phosphate
show the reaction diagram
-
31% of the activity with 4-nitrophenylphosphate
-
-
?
dCTP + H2O
dCDP + phosphate
show the reaction diagram
-
367% relative activity compared to myo-inositol hexakisphosphate
-
-
?
dCTP + H2O
? + phosphate
show the reaction diagram
-
35% of the activity with 4-nitrophenylphosphate
-
-
?
dGTP + H2O
dGDP + phosphate
show the reaction diagram
-
189% relative activity compared to myo-inositol hexakisphosphate
-
-
?
dGTP + H2O
? + phosphate
show the reaction diagram
-
52% of the activity with 4-nitrophenylphosphate
-
-
?
di-sodium phenyl phosphate dihydrate + H2O
?
show the reaction diagram
-
111.85% activity compared to myo-inositol hexakisphosphate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
-
-
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
438.5% of the activity with myo-inositol hexakisphosphate
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
95% of the activity with 4-nitrophenylphosphate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
Na-diphosphate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
Klebsiella oxytoca MO-3
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
Na-diphosphate
-
-
?
dTTP + H2O
dTDP + phosphate
show the reaction diagram
-
389% relative activity compared to myo-inositol hexakisphosphate
-
-
?
dTTP + H2O
? + phosphate
show the reaction diagram
-
37% of the activity with 4-nitrophenylphosphate
-
-
?
fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
-
-
-
?
fructose 1,6-diphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
fructose 1,6-diphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
fructose 1,6-diphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
fructose 1,6-diphosphate + H2O
?
show the reaction diagram
Klebsiella sp., Klebsiella sp. ASR1
-
12.3% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
glucose 1-phosphate + H2O
glucose + phosphate
show the reaction diagram
-
-
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
-
-
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
-
-
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
-
-
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
Klebsiella sp., Klebsiella sp. ASR1
-
2.7% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
Klebsiella oxytoca MO-3
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
50.8% of the activity with myo-inositol hexakisphosphate
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
85.6% of the activity with myo-inositol hexakisphosphate
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
Klebsiella oxytoca MO-3
-
less than 10% of the activity with myo-inositol hexakisphosphate
-
-
?
glycerol 3-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
glycerol 3-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
43% of the activity with 4-nitrophenylphosphate
-
-
?
GTP + H2O
?
show the reaction diagram
-
-
-
?
GTP + H2O
?
show the reaction diagram
-
-
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
-
271% relative activity compared to myo-inositol hexakisphosphate
-
-
?
inositol 1-monophosphate + H2O
inositol + phosphate
show the reaction diagram
-
58% activity compared to myo-inositol hexakisphosphate
-
-
?
inositol 2-monophosphate + H2O
inositol + phosphate
show the reaction diagram
-
84% activity compared to myo-inositol hexakisphosphate
-
-
?
inositol bisphosphate + H2O
?
show the reaction diagram
Klebsiella sp., Klebsiella sp. PG-2
-
-
-
-
?
inositol monophosphate + H2O
inositol + phosphate
show the reaction diagram
Klebsiella sp., Klebsiella sp. PG-2
-
-
-
-
?
inositol pentaphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
inositol tetraphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
inositol tetraphosphate + H2O
?
show the reaction diagram
-
almost the same activity as with myo-inositol hexakisphosphate
-
-
?
inositol triphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
inositol triphosphate + H2O
?
show the reaction diagram
-
40% of the activity with myo-inositol hexakisphosphate
-
-
?
myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
myo-inositol-2,4,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens DS11
-
-
-
-
?
myo-inositol 2,4,5,6-tetrakisphosphate + H2O
myo-inositol 2,4,6-trisphosphate + phosphate
show the reaction diagram
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens DS11
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
inositol + phosphate
show the reaction diagram
Klebsiella sp., Klebsiella sp. PG-2
-
-
-
-
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
O00093
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
P34752
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
O00107
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Talaromyces thermophilus
O00096
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
A2TBB4
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Schwanniomyces castellii, Paramecium sp.
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
O00092
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Q6T9Z6
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
-
a mixture of two pentaphosphates: the major component is 1-myo-inositol 1,2,4,5,6-pentakisphosphate and the other is 1-myo-inositol 1,2,3,4,5-pentakisphosphate
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
about 85% of myo-inositol hexakisphosphate in soybean meal is hydrolyzed by the enzyme whereas only 67% of the myo-inositol hexakisphosphate in cottonseed meal is hydrolyzed by the same enzyme treatment
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
100% relative activity
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
-
100% activity
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Cryptococcus laurentii, Cryptococcus laurentii ABO 510
-
preferred substrate, 100% activity
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger NRRL 4361, Aspergillus niger NRRL 326
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger 92
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Selenomonas ruminantium JY35
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Bacillus amyloliquefaciens DSM7
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus fumigatus WY-2
O00092
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Myceliophthora heterothallica BJTLR50
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus ficuum WB 4541
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Saccharomyces cerevisiae CY
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger N-J
Q6T9Z6
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus terreus CBS
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger NRRL 3135
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Bacillus subtilis VTT E-68013
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger IIIAn/8
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus terreus 9A1
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus tubingensis NRRL 4875, Aspergillus ficuum WB 4016, Aspergillus niger NRRL 330, Aspergillus ficuum X, Aspergillus ficuum K
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Klebsiella sp. ASR1
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus ficuum WB 320
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Rhizopus microsporus var. oligosporus NRRL 2710, Aspergillus niger NRRL 65
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Kodamaea ohmeri BG3
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus ficuum WB 364, Aspergillus niger NRRL337, Aspergillus ficuum NRRL 3135
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus ficuum NRRL 3135
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger NRRL372, Aspergillus niger NRRL 372, Aspergillus ficuum WB4781
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Aspergillus niger T213
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate
show the reaction diagram
Klebsiella oxytoca MO-3
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
-
-
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
O93838, P34752
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
O00085
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Talaromyces thermophilus
O00096
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
O00093
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
O00092
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Q8KTX7
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Q6U677
-
-
-
-
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Schwanniomyces castellii
-
constitutive
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
hydrolyzes phytate in a stepwise manner
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
product release is the rate limiting step of the reaction
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
rapid equilibrium ordered mechanism in which binding of Ca2+ to the active site is necessary for the essential activation of the enzyme. Ca2+ turns out to be also required for the substrate because the enzyme is only able to hydrolyze the calcium-phythate complex
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
step-wise hydrolysis of myo-inositol hexakisphosphate
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
the enzyme is able to hydrolyze any of the six phosphate groups of phytate. The reaction is likely to proceed through a direct attack of the metal-bridging water molecule on the phosphorous atom of a substrate and the subsequent stabilization of the pentavalent transition state by the bound calcium ions
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
very specific for phytate
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
very specific for, no activity on other phosphate esters
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
inducible enzyme
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
P34753
constitutive enzyme
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
inducible
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate (i.e. myo-inositol 1,2,3,4,5-pentakisphosphate) or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Aspergillus terreus CBS
O00085
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Aspergillus niger T213.
-
product release is the rate limiting step of the reaction
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Saccharomyces cerevisiae BY85.
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate (i.e. myo-inositol 1,2,3,4,5-pentakisphosphate) or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Bacillus sp. KHU-10.
-
very specific for, no activity on other phosphate esters
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Aspergillus niger NRRL3135.
-
product release is the rate limiting step of the reaction
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Bacillus sp. DS1.
-
-, constitutive enzyme
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Bacillus amyloliquefaciens S11.
-
rapid equilibrium ordered mechanism in which binding of Ca2+ to the active site is necessary for the essential activation of the enzyme. Ca2+ turns out to be also required for the substrate because the enzyme is only able to hydrolyze the calcium-phythate complex
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Pseudomonas syringae MOK1.
-
very specific for phytate
-
?
myo-inositol hexakisphosphate + H2O
myo-inositol pentakisphosphate
show the reaction diagram
-
-
-
?
myo-inositol hexakisphosphate + H2O
D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
-
-
after 10 min D-myo-inositol 1,2,4,5,6-pentakisphosphate is the major degradation product, accompanied by small amounts of D-myo-inositol 1,2,3,4,6-pentakisphosphate, D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate. After 30 min, the quantity of D-myo-inositol 1,2,4,5,6-pentakisphosphate decreases and the levels of D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate increases. After 90 min the major products are Ins(1,3,5) P3 and Ins(2,4,6)P3
?
myo-inositol hexakisphosphate + H2O
D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
-
step-wise dephosphorylation occurs via 1. D-myo-inositol 1,2,4,5,6-pentakisphosphate, 2. D-myo-inositol 1,2,5,6-tetrakisphosphate or D-myo-inositol 2,4,5,6-tetrakisphosphate, 3. D-myo-inositol 1,2,6-trisphosphate, D-myo-inositol 1,2,3-trisphosphate or D-myo-inositol 1,4,5-trisphosphate, 4. myo-inositol 1,2-bisphosphate or myo-inositol 2,5-bisphosphate, myo-inositol 4,5-bisphosphate or myo-inositol 2,4-bisphosphate. Myo-inositol 2-monophosphate is the final product
-
?
myo-inositol hexakisphosphate + H2O
D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
Bacillus subtilis VTT E-68013
-
-
after 10 min D-myo-inositol 1,2,4,5,6-pentakisphosphate is the major degradation product, accompanied by small amounts of D-myo-inositol 1,2,3,4,6-pentakisphosphate, D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate. After 30 min, the quantity of D-myo-inositol 1,2,4,5,6-pentakisphosphate decreases and the levels of D-myo-inositol 2,4,5,6-tetrakisphosphate and D-myo-inositol 1,2,35-tetrakisphosphate increases. After 90 min the major products are Ins(1,3,5) P3 and Ins(2,4,6)P3
?
myo-inositol hexakisphosphate + H2O
myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens DS11
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Q84B22
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
P34752
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
A5A5J1
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
B4X9S4
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Q7CIZ7
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Eupenicillium parvum
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
sodium salt
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Cryptococcus laurentii AL27
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Aspergillus niger NRRL 3135
-, P34752
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
Eupenicillium parvum BCC1769
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
A5A5J1
-
-
-
?
myo-inositol hexakisphosphate + H2O
myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
7% of the activity with 4-nitrophenylphosphate
-
-
?
myo-inositol hexakisphosphate + H2O
myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
show the reaction diagram
-
sodium salt
-
-
?
myo-inositol hexakisphosphate disodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
B3VPB2
-
-
-
?
myo-inositol hexakisphosphate disodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
B3VPB3
-
-
-
?
myo-inositol hexakisphosphate disodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
Aspergillus japonicus BCC18313
B3VPB2
-
-
-
?
myo-inositol hexakisphosphate disodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
Aspergillus niger BCC18081
B3VPB3
-
-
-
?
myo-inositol hexakisphosphate sodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
B3VPB2
-
-
-
?
myo-inositol hexakisphosphate sodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
B3VPB3
-
-
-
?
myo-inositol hexakisphosphate sodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
Aspergillus japonicus BCC18313
B3VPB2
-
-
-
?
myo-inositol hexakisphosphate sodium salt + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate
show the reaction diagram
Aspergillus niger BCC18081
B3VPB3
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-, P34752
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
D3HIF3, -
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
D9D7K9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKK7, C4PKK9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKL2, C4PKL4
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKL6
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
cleavage initiation in the dephosphorylation of myo-inositol hexakisphosphate at C3
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
substrate Na-phytate
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Bacillus subtilis CF92
-
-, substrate Na-phytate
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger NRRL 3135
-, P34752
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger 113
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger FS3
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
myo-inositol-1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
Q84CN9
although the PhyK homolog AppA is biochemically characterized as a 6-phytase, a co-crystal structure shows the phytate 3-phosphate as scissile group in a similar position to in the active site of PhyK
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
1D-myo-inositol-1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
Q84CN9
although the PhyK homolog AppA is biochemically characterized as a 6-phytase, a co-crystal structure shows the phytate 3-phosphate as scissile group in a similar position to in the active site of PhyK
-
-
?
NADP+ + H2O
NAD+ + phosphate
show the reaction diagram
-
58.1% of the activity with myo-inositol hexakisphosphate, recombinant enzyme
-
?
NADP+ + H2O
?
show the reaction diagram
-
-
-
-
?
naphthyl 1-phosphate + H2O
naphthol + phosphate
show the reaction diagram
-
-
-
-
?
O-phospho-L-serine + H2O
L-serine + phosphate
show the reaction diagram
-
-
-
?
O-phospho-L-serine + H2O
L-serine + phosphate
show the reaction diagram
-
25% of the activity with 4-nitrophenylphosphate
-
-
?
O-phospho-L-tyrosine + H2O
L-tyrosine + phosphate
show the reaction diagram
-
28% of the activity with 4-nitrophenylphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Q6T9Z6
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
135% activity compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
152.95% activity compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
311% relative activity compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Cryptococcus laurentii ABO 510
-
152.95% activity compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Schwanniomyces capriottii CBS 2923
-
135% activity compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Bacillus amyloliquefaciens DSM7
-
the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Aspergillus niger N-J
Q6T9Z6
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Klebsiella sp. ASR1
-
the enzyme shows 22.5% activity towards p-nitrophenyl phosphate compared to myo-inositol hexakisphosphate
-
-
?
phenyl phosphate
phenol + phosphate
show the reaction diagram
-
-
-
?
phenyl phosphate
phenol + phosphate
show the reaction diagram
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
-
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
-
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
-
84% of the activity with myo-inositol hexakisphosphate
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
-
89.2% of the activity with myo-inositol hexakisphosphate
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
-
188% relative activity compared to myo-inositol hexakisphosphate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
-
130% activity compared to myo-inositol hexakisphosphate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
-
328% relative activity compared to myo-inositol hexakisphosphate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
-
92% of the activity with 4-nitrophenylphosphate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
Schwanniomyces capriottii CBS 2923
-
130% activity compared to myo-inositol hexakisphosphate
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
Q0GYS1, Q0GYS2
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
Na- and Ca-phytate, the first results in higher activity, substrate binding analysis by molecular modelling of phytate inside the active site, residue K77 is able to form two hydrogen bonds with the two phosphate groups on the phytate, whereas in case of K77R, the guanidinium group of arginine is able to form up to 4 H-bonds with the two phosphate groups
-
-
?
phytate + H2O
?
show the reaction diagram
-
-, Na- and Ca-phytate, the first results in higher activity, substrate binding analysis by molecular modelling of phytate inside the active site, residue K77 is able to form two hydrogen bonds with the two phosphate groups on the phytate, whereas in case of K77R, the guanidinium group of arginine is able to form up to 4 H-bonds with the two phosphate groups
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
Q6RK08
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
G4WZU3, G4WZU4
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
Serratia sp. TN49, ACCC 03909
G4WZU3, G4WZU4
-
-
-
?
pyridoxal phosphate + H2O
pyridoxal + phosphate
show the reaction diagram
-
-
-
?
riboflavin 5-phosphate + H2O
riboflavin + phosphate
show the reaction diagram
-
-
-
-
?
soybean meal + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
tetrasodium diphosphate + H2O
?
show the reaction diagram
-
6% of the activity with myo-inositol hexakisphosphate
-
?
UTP + H2O
UDP + phosphate
show the reaction diagram
-
350% relative activity compared to myo-inositol hexakisphosphate
-
-
?
L-tyrosine phosphate + H2O
L-tyrosine + phosphate
show the reaction diagram
-
142% relative activity compared to myo-inositol hexakisphosphate
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
enzyme exhibits phytase and acid phosphatase activity
-
?
additional information
?
-
-
less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate
-
?
additional information
?
-
-
no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP
-
?
additional information
?
-
-
no activity with GTP and diphosphate
-
?
additional information
?
-
-
inducible enzyme
-
-
-
additional information
?
-
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
-
high levels of phosphorous in the growth medium inhibit phytase synthesis, effect is larger in semisolid medium than in liquid medium
-
-
-
additional information
?
-
-
the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate
-
-
-
additional information
?
-
-
role of phytase in Ca2+ mobilization during germination of mung bean seed via a salvage pathway that involves allosteric activation by myo-inositol triphosphate
-
?
additional information
?
-
-
degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
-
additional information
?
-
-
phytases from bifidobacteria could be partly active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
-
phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
-
no activity towards 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate and 1-L-myo-inositol 1,2,5,6-tetrakisphosphate
-
-
-
additional information
?
-
-
to reduce phytates in dried grains with solubles (DDGS) and corn gluten feed (CGF), a phytase from Aspergillus niger, PhyA, is investigated regarding its capability to catalyze the hydrolysis of phytates in light steep water and whole stillage. Dephosphorylation of phytates in light steep water and whole stillage proceeds via the formation of InsP5, InsP4, InsP3, and InsP2 intermediates with phosphate and InsP1 as the end products. During the process, the amount of phosphate in the substrates is increased from 54% to 66% in the whole stillage, and from 20% to 90% in the light steep water, suggesting to a substantial dephosphorylation of the phytates in the light steep water and whole stillage via PhyA catalyzed hydrolysis. The most effective period of degradation is during the first 2 h for whole stillage and 6 h for light steep water
-
-
-
additional information
?
-
C4PKK7, C4PKK9
substrate specificity of recombinant isozymes, overview
-
-
-
additional information
?
-
C4PKL2, C4PKL4
substrate specificity of recombinant isozymes, overview
-
-
-
additional information
?
-
B3VPB3
no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s
-
-
-
additional information
?
-
B3VPB2
no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s
-
-
-
additional information
?
-
-
phytase hydrolyzes and liberates inorganic phosphate from Ca2+, Mg2+, and Co2+ phytates more efficiently than those of Al3+, Fe2+, Fe3+, and Zn2+
-
-
-
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, overview
-
-
-
additional information
?
-
-
HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+
-
-
-
additional information
?
-
Q84CN9
phytase ASr1 acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule
-
-
-
additional information
?
-
D3HIF3, -
the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents
-
-
-
additional information
?
-
G4WZU3, G4WZU4
phytase activity is determined using the ferrous sulfate-molybdenum blue method
-
-
-
additional information
?
-
Q84CN9
substrate binding structure modelling, all six phosphate groups of phytate are involved in a hydrogen bond network connecting the substrate with PhyK, induced conformational changes upon substrate binding, overview
-
-
-
additional information
?
-
-
substrate specificity of the incomplete domain and the functional relationship of tandemly repeated domains in beta-propeller phytases. The incomplete domain Phy-DI is not functional with myo-inositol-1,2,3,4,5,6-hexakisphosphate at 35C and pH 7.0
-
-
-
additional information
?
-
C4PKL6
substrate specificity of the recombinant isozyme, overview
-
-
-
additional information
?
-
-
substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview
-
-
-
additional information
?
-
D3HIF3
the enzyme catalyzes the dephytinization of soymilk
-
-
-
additional information
?
-
-
the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate
-
-
-
additional information
?
-
-
the native enzyme is also active on ADP, ATP, alpha- and beta-glycerophosphate, 2-naphthyl phosphate, and 4-nitrophenyl phosphate. No activity with sodium tripolyphosphate, fructose-6-phosphate, diphosphate, AMP
-
-
-
additional information
?
-
D3HIF3, -
the phytase exhibits broad substrate specificity since it hydrolyses 4-nitrophenyl phosphate, ATP, ADP, and glucose-6-phosphate besides phytic acid. The enzyme hydrolyzes insoluble calcium and magnesium phytates efficiently, but not iron phytate
-
-
-
additional information
?
-
-
the phytase isozymes exhibit a broad affinity for various phosphorylated compounds, i.e. 4-nitrophenyl phosphate 1-naphthyl phosphate, 2-naphthyl phosphate, AMP, ATP, GTP, fructose-1, 6-bisphosphate, fructose-6-phosphate, glucose-6-phosphate, and pyridoxal phosphate, substrate specificities, overview
-
-
-
additional information
?
-
Q0GYS1, Q0GYS2
the substrate specificity of LlALP from lily pollen is unique among phytases, it differs from that of acid phytases, which show broad substrate specificity, and from other alkaline phytases, which exhibit narrow substrate specificity including lack of activity against 4-nitrophenyl phosphate. LlALP from lily pollen hydrolyzes phytate and pNPP
-
-
-
additional information
?
-
Q0GYS1, Q0GYS2
the substrate specificity of LlALP from lily pollen is unique among phytases, it differs from that of acid phytases, which show broad substrate specificity, and from other alkaline phytases, which exhibit narrow substrate specificity including lack of activity against 4-nitrophenyl phosphate. LlALP from lily pollen hydrolyzes phytate and pNPP. The recombinant LlALP2 expressed in Pichia pastoris hydrolyzes phytate and 4-nitrophenyl phosphate and exhibits no activity towards ATP. Activity against 4-nitrophenyl phosphate is nearly 2.5fold higher than against phytate, similar to what is observed with the native enzyme
-
-
-
additional information
?
-
Aspergillus japonicus BCC18313
B3VPB2
no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s
-
-
-
additional information
?
-
Serratia sp. TN49, ACCC 03909
G4WZU3, G4WZU4
phytase activity is determined using the ferrous sulfate-molybdenum blue method
-
-
-
additional information
?
-
Bacillus subtilis CF92
-
the native enzyme is also active on ADP, ATP, alpha- and beta-glycerophosphate, 2-naphthyl phosphate, and 4-nitrophenyl phosphate. No activity with sodium tripolyphosphate, fructose-6-phosphate, diphosphate, AMP
-
-
-
additional information
?
-
Aspergillus niger NRRL 4361, Aspergillus niger NRRL 326
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum WB 4541
-
-
-
-
-
additional information
?
-
Aspergillus ficuum NTG-23
-
the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate
-
-
-
additional information
?
-
Aspergillus ficuum WB 4016
-
-
-
-
-
additional information
?
-
Saccharomyces cerevisiae BY85.
-
degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
-
additional information
?
-
Aspergillus niger NRRL 330
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
substrate specificity of recombinant isozymes, overview
-
-
-
additional information
?
-
Aspergillus ficuum X, Aspergillus ficuum K
-
-
-
-
-
additional information
?
-
Klebsiella sp. PG-2
-
inducible enzyme
-
-
-
additional information
?
-
Klebsiella sp. ASR1
-
no activity with GTP and diphosphate
-
?
additional information
?
-
Aspergillus ficuum WB 320
-
-
-
-
-
additional information
?
-
Citrobacter braakii YH-15
-
less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate
-
?
additional information
?
-
Aspergillus niger FS3
-
substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview
-
-
-
additional information
?
-
Bacillus sp. KHU-10.
-
no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP
-
?
additional information
?
-
Aspergillus ficuum WB 364
-
-
-
-
-
additional information
?
-
-
substrate specificity of the incomplete domain and the functional relationship of tandemly repeated domains in beta-propeller phytases. The incomplete domain Phy-DI is not functional with myo-inositol-1,2,3,4,5,6-hexakisphosphate at 35C and pH 7.0
-
-
-
additional information
?
-
Aspergillus niger NRRL337
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum NRRL 3135
-
-
-
-
-
additional information
?
-
Aspergillus niger NRRL372
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Bifidobacterium adolescentis ATCC15703
-
phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
Aspergillus niger NRRL 372
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum WB4781
-
-
-
-
-
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, overview
-
-
-
additional information
?
-
Aspergillus niger BCC18081
B3VPB3
no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Schwanniomyces castellii
-
constitutive
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
inducible enzyme
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
P34753
constitutive enzyme
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
-
inducible
-
?
myo-inositol hexakisphosphate + H2O
?
show the reaction diagram
Bacillus sp. DS1.
-
constitutive enzyme
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-, P34752
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
D3HIF3, -
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
D9D7K9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKK7, C4PKK9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKL2, C4PKL4
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
C4PKL6
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
cleavage initiation in the dephosphorylation of myo-inositol hexakisphosphate at C3
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
myo-inositol-1,2,4,5,6-pentakisphosphate + phosphate
show the reaction diagram
Q84CN9
although the PhyK homolog AppA is biochemically characterized as a 6-phytase, a co-crystal structure shows the phytate 3-phosphate as scissile group in a similar position to in the active site of PhyK
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Bacillus subtilis CF92
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger NRRL 3135
-, P34752
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger 113
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Triticum aestivum Bob white SH 98 26
C4PKK7, C4PKK9
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
Aspergillus niger FS3
-
-
-
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
phytate + H2O
?
show the reaction diagram
Q0GYS1, Q0GYS2
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
Q6RK08
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
G4WZU3, G4WZU4
-
-
-
?
phytate + H2O
? + phosphate
show the reaction diagram
Serratia sp. TN49, ACCC 03909
G4WZU3, G4WZU4
-
-
-
?
phytate + H2O
?
show the reaction diagram
-
-
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
inducible enzyme
-
-
-
additional information
?
-
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
-
high levels of phosphorous in the growth medium inhibit phytase synthesis, effect is larger in semisolid medium than in liquid medium
-
-
-
additional information
?
-
-
the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate
-
-
-
additional information
?
-
-
role of phytase in Ca2+ mobilization during germination of mung bean seed via a salvage pathway that involves allosteric activation by myo-inositol triphosphate
-
?
additional information
?
-
-
degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
-
additional information
?
-
-
phytases from bifidobacteria could be partly active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
-
phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
-
HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+
-
-
-
additional information
?
-
Q84CN9
phytase ASr1 acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule
-
-
-
additional information
?
-
D3HIF3, -
the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents
-
-
-
additional information
?
-
Aspergillus niger NRRL 4361, Aspergillus niger NRRL 326
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum WB 4541, Aspergillus ficuum WB 4016
-
-
-
-
-
additional information
?
-
Saccharomyces cerevisiae BY85.
-
degradation of phytate by high-phytase Saccharomyces cerevisiae strains during simulated gastrointestinal digestion. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
-
-
additional information
?
-
Aspergillus niger NRRL 330
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum X, Aspergillus ficuum K
-
-
-
-
-
additional information
?
-
Klebsiella sp. PG-2
-
inducible enzyme
-
-
-
additional information
?
-
Aspergillus ficuum WB 320, Aspergillus ficuum WB 364
-
-
-
-
-
additional information
?
-
Aspergillus niger NRRL337
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum NRRL 3135
-
-
-
-
-
additional information
?
-
Aspergillus niger NRRL372
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Bifidobacterium adolescentis ATCC15703
-
phytases from bifidobacteria partly could be active in human gut and could contribute to phytic acid degradation during food processing
-
-
-
additional information
?
-
Aspergillus niger NRRL 372
-
the enzyme production is strongly repressed by inorganic phosphates and requires a high carbon to phosphorus ratio in the medium
-
-
-
additional information
?
-
Aspergillus ficuum WB4781
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
53% activation at 1 mM, 46% activation at 20 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Ba2+
-
19.5% stimulatory effect at 5 mM
Ca2+
-
0.2 mM, 12% increase of activity
Ca2+
-
activates
Ca2+
-
activates
Ca2+
-
rapid equilibrium ordered mechanism in which binding of ca2+ to the active site is necessary for the essential activation of the enzyme. Ca2+ turns out to be also required for the substrate because the enzyme is only able to hydrolyze the calcium-phytate complex. 4 mol of Ca2+ bind to 1 mol of phytate
Ca2+
-
the crystal structure of the enzyme in complex with inorganic phosphate reveals that two phosphates and four calcium ions are tightly bound at the active site
Ca2+
-
45% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Ca2+
-
1.0 mM, required for activity
Ca2+
O00092
1 mM, slight stimulatory effect
Ca2+
-
10.8% stimulatory effect at 5 mM
Ca2+
A5A5J1
required both for refolding and activity
Ca2+
-
127% of initial activity
Ca2+
-
phytase hydrolyzes and liberates inorganic phosphate from Ca2+, Mg2+, and Co2+ phytates more efficiently than those of Al3+, Fe2+, Fe3+, and Zn2+
Ca2+
O31097
stabilizes the recombinant and the native enzyme
Ca2+
O31097, P42094, Q70E78, Q84B22, Q93GB6, Q9F657
activates; activates; activates; activates; activates; activates
Ca2+
Janthinobacterium sp.
G0XZM1
activates, required, optimal at 1.5-2.5 mM, activity profile, overview
Ca2+
-
required, best at 3 mM
Ca2+
-
activates
Ca2+
-
the purified recombinant enzyme domains PhyH and PhyH-DII require Ca2+ for phytase activity, optimal at 1 mM
Ca2+
Q0GYS1, Q0GYS2
activates the recombinant isozyme LlALP2 4fold at 1 mM; activates the recombinant isozyme LlALP2 4fold at 1 mM
Ca2+
A4UU76
stabilizes at 1 mM, activates 20% at 1 mM, 12% at 5 mM
Cd2+
-
200 mM, activation to 156% of control
Co2+
-
35% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Co2+
O00092
1 mM, slight stimulatory effect
Co2+
-
25.5% stimulatory effect at 5 mM
Co2+
-
phytase hydrolyzes and liberates inorganic phosphate from Ca2+, Mg2+, and Co2+ phytates more efficiently than those of Al3+, Fe2+, Fe3+, and Zn2+
Co2+
Eupenicillium parvum
-
5 mM, 112% of initial activity
Cu2+
-
200 mM, activation to 160% of control
Fe2+
-
13% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Fe2+
C4PKK7, C4PKK9
activates isozyme b1 about 5fold at 10 mM
Hg2+
-
200 mM, activation to 158% of control
Hg2+
-
13% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
K+
-
40% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
K+
O00092
1 mM, slight stimulatory effect
K+
-
14.9% stimulatory effect at 5 mM
Li+
-
17.5% stimulatory effect at 5 mM
Mg2+
-
0.2 mM, 6% increase of activity
Mg2+
-
200 mM, activation to 154% of control
Mg2+
O00092
1 mM, slight stimulatory effect
Mg2+
-
21.7% stimulatory effect at 5 mM
Mg2+
-
phytase hydrolyzes and liberates inorganic phosphate from Ca2+, Mg2+, and Co2+ phytates more efficiently than those of Al3+, Fe2+, Fe3+, and Zn2+
Mg2+
-
0.5 mM, 11% increase in activity
Mn2+
-
0.2 mM, 59% increase of activity
Mn2+
-
activates
Mn2+
-
200 mM, activation to 147.8% of control
Mn2+
O00092
1 mM, slight stimulatory effect
Mn2+
-
17.8% stimulatory effect at 5 mM
Na+
-
14.8% stimulatory effect at 5 mM
Pb2+
-
5 mM, enhances activity
vanadate
-
incubation with vanadate to obtain a biocatalyst in the asymmetric sulfoxidation of thioanisole using hydrogen peroxide. Vanadate is coordinatecd to oxygen functions at two different binding sites, and the alpha-helical content decreases upon coordination of vanadate
Zn2+
-
activates
Zn2+
-
activates
Zn2+
-
200 mM, activation to 142.7% of control
Zn2+
-
13% activation at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Zn2+
-
129.4%, of initial activity
Mn2+
C4PKK7, C4PKK9
activates isozyme a1 12fold at 10 mM
additional information
-
no effect is observed after addition of 200 mM Fe2+ or Fe3+
additional information
-
not affected at concentrations up to 10 mM by: Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+
additional information
-
phytase LP11, LP12 and LP2 are not metalloenzymes
additional information
-
Li+, Na+, K+ and Mg2+ do not significantly affect the phytase activity
additional information
A5A5J1
enzyme refolded in presence of Cu2+, Fe2+, Mg2+, Co2+, Zn2+ or Mn2+, fails to show any enzyme activity even in the presence of Ca2+ in the reaction mix
additional information
-
no activation or inhibition by cations K+, Ca2+, Mg2+, Mn2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Fe3+, and Al3+; no effect: Ca2+, Mg2+, Mn2+, Zn2+
additional information
D3HIF3
no requirement of metal ions for the activity
additional information
-
the enzyme is inhibited by metal ions Mn2+, Zn2+, Ca2+, Cu2+, Fe2+, Mg2+, and Co2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,2-Cyclohexanedione
-
about 7 Arg of the total 19 are modified, partially reversed by 0.2 M hydroxylamine
1,2-Cyclohexanedione
-
; 2 mM, 50% loss of activity
2,3-Butanedione
D3HIF3
-
2,4-Dinitrophenol
-
-
2,4-Dinitrophenol
-
-
Ag+
-
77.12% residual activity at 5 mM
Ag+
-
49% inhibitory effect at 5 mM
Ag+
-
1 mM, 25% of initial activity
Ag+
G4WZU3, G4WZU4
;
Al3+
-
98% inhibition at 1 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Al3+
O00092
1 mM, inhibitive effect
arsenate
-
the enzyme of Pteris vittata shows partial resistance to arsenate in contrast to enzymes from other ferns, e.g. Pteris ensiformis and Nephrolepis exaltata
ATP
-
substrate inhibition above 5 mM
Ba2+
-
71.95% residual activity at 5 mM
Ba2+
A4UU76
25% inhibition at 1 mM, 44% inhibition at 5 mM
Ca2+
-
0.1-1 mM, slight inhibition
Ca2+
-
free, not complexed with phytate, competitive inhibitor
Ca2+
-
200 mM, 32% inhibition
Ca2+
-
5 mM, 10% decreased activity, although calcium has no direct influence on enzymatic activity, it plays a crucial protective role, since in the absence of calcium, the denaturation of the enzyme is complete after 1 h at 66.5C
Ca2+
-
decreases activity below 0.1 mM or above 2 mM
Ca2+
-
68.64% residual activity at 5 mM
Cd2+
-
5 mM, 70% inhibition
Cd2+
-
40.54% residual activity at 1 mM
Cd2+
A4UU76
92% inhibition at 1 mM, 98% inhibition at 5 mM
Co2+
-
0.1 mM-1 mM, slight inhibition
Co2+
-
60% inhibition at 20 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Co2+
-
76.08% residual activity at 5 mM
Cr3+
-
1 mM, 25% of initial activity
Cr3+
G4WZU3, G4WZU4
;
Cu2+
-
0.1-1 mM, strong inhibition
Cu2+
-
5 mM, 70% inhibition
Cu2+
-
0.1 mM-1 mM, strong inhibition
Cu2+
-
effect on the ability of phytase to hydrolyze phytate phosphorus is dependent on pH. At pH 2.5 (gastric pH), no inhibition is noted among the treatments (copper citrate, copper chloride, copper lysinate, cupper sulfate, tribasic copper chloride) except that the addition of 250 and 500 mg Cu/kg diet from copper chloride and 500 mg Cu/kg from copper sulfate slightly inhibits phytate phosphorus hydrolysis. When pH is increased to 5.5-6.5 (small intestinal digesta) phytate phosphorus hydrolysis is greatly inhibited
Cu2+
-
70% inhibition at 5 mM, 95% inhibition at 15 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Cu2+
O00092
1 mM, inhibitive effect
Cu2+
-
14.03% residual activity at 5 mM
Cu2+
-
17.5% inhibitory effect at 5 mM
Cu2+
-
1 mM, 3% of initial activity
Cu2+
-
17.8% of initial activity
Cu2+
-
61% residual activity
Cu2+
-
0.5 mM, 90% decrease in activity
Cu2+
Eupenicillium parvum
-
5 mM, 50% inhibition
Cu2+
A4UU76
32% inhibition at 1 mM, 49% inhibition at 5 mM
CuSO4
-
2.5 mM, 70% inhibition
DTT
A4UU76
6% inhibition at 5 mM
EDTA
-
5 mM, 70% inhibition
EDTA
-
1 mM, 94% inhibition
EDTA
A5A5J1
-
EDTA
Q84B22
1 mM, complete inhibition
EDTA
G4WZU3, G4WZU4
-
EDTA
Q0GYS1, Q0GYS2
;
EDTA
A4UU76
94% inhibition at 1 mM, 100% inhibition at 5 mM
F-
-
1 mM, 49% inhibition. 2.5 mM, 61% inhibition
F-
-
uncompetitive
Fe2+
-
0.1 mM-1 mM, strong inhibition
-
Fe2+
-
59% inhibition at 5-15 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
-
Fe2+
-
5 mM, 10% decreased activity
-
Fe2+
-
41.3% residual activity at 5 mM
-
Fe2+
-
25.7% inhibitory effect at 5 mM
-
Fe2+
-
stronger inhibition at 10 mM of isozyme RO2 compared to isozyme RO1
-
Fe3+
-
0.1 mM-1 mM, strong inhibition
-
Fe3+
-
2.5 mM, 68% inhibition
-
Fe3+
O00092
1 mM, inhibitive effect
-
Fe3+
-
19.4% inhibitory effect at 5 mM
-
Fe3+
-
1 mM, 2.5% of initial activity
-
Fe3+
-
complete inhibition
-
Fe3+
-
0.5 mM, 99% decrease in activity
-
Fe3+
G4WZU3, G4WZU4
;
-
Fe3+
-
stronger inhibition at 10 mM of isozyme RO2 compared to isozyme RO1
-
goethite
-
loss of activity upon sorption on, overview
-
guanidine hydrochloride
D3HIF3
-
guanidinium-HCl
-
6 M, 92% inhibition. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
haematite
-
loss of activity upon sorption on, overview
-
Hg2+
-
48% inhibition at 10 mM, 87% inhibition at 20 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Hg2+
-
31.17% residual activity at 5 mM
Hg2+
-
99.2% inhibitory effect at 5 mM
Hg2+
-
1 mM, 0.6% of initial activity
Hg2+
-
10% residual activity
iodoacetic acid
-
-
iodoacetic acid
-
6.3% inhibitory effect at 1 mM
K+
-
16% inhibition at 20 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
kaolinite
-
loss of activity upon sorption on, overview
-
KH2PO4
-
complete inhibition at 0.5 mM
KI
-
6 M, 95% inhibition. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Li+
A4UU76
10% inhibition at 1 mM, 13% inhibition at 5 mM
metavanadate
-
50% inhibition at 0.0011 and pH 2.5, or at 0.0053 mM and pH 5.0
-
metavanadate
Q6RK08
50% inhibition at 0.00075 mM and pH 5.0 or at 0.00090 mM and pH 3.0
-
Mg2+
-
61% inhibition at 5 mM, 92% inhibition at 15 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Mg2+
G4WZU3, G4WZU4
-
Mg2+
A4UU76
18% inhibition at 1 mM, 27% inhibition at 5 mM
MgCl2
-
2.5 mM, 20% inhibition
Mn2+
-
0.1-1 mM, slight inhibition
Mn2+
-
5 mM, 70% inhibition
Mn2+
-
0.1 mM-1 mM, slight inhibition
Mn2+
-
73% inhibition at 4 mM, 97% inhibition at 15 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Mn2+
-
5 mM, 71% decreased activity
Mn2+
-
36.01% residual activity at 5 mM
Mn2+
G4WZU3, G4WZU4
;
Mn2+
A4UU76
49% inhibition at 1 mM, 67% inhibition at 5 mM
MnCl2
-
2.5 mM, 17% inhibition
molybdate
-
-
molybdate
-
95% inhibition at 0.0625 mM
molybdate
-
almost complete inhibition of isozymes RO1 and RO2 at 1-10 mM
myo-inositol hexakisphosphate
-
inhibition at substrate concentrations above 2.5 mM
myo-inositol hexakisphosphate
-
completely inhibited by 5 mM myo-inositol hexakisphosphate
myo-inositol hexasulfate
-
; 0.01 mM, 50% inhibition
myo-inositol hexasulfate
-
-
myo-inositol hexasulfate
-
0.02 mM, 90% inhibition, recombinant enzyme
myo-inositol hexasulfate
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate (i.e. myo-inositol 1,2,3,4,5-pentakisphosphate) or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
myo-inositol-1,2,3,4,5,6-hexakissulfate
-
potent inhibition, the compound is isosteric and isoelectronic with respect to substrate myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
Na2MoO4
-
2.5 mM, 87% inhibition
Na3VO4
-
0.1 m, 79% inhibition. 2.5 mM, 90% inhibition
Ni(2+)
-
79.25% residual activity at 5 mM
Ni2+
-
44% inhibition at 20 mM
Ni2+
G4WZU3, G4WZU4
-
orthovanadate
-
50% inhibition at 0.0004 and pH 2.5, or at 0.040 mM and pH 5.0
orthovanadate
Q6RK08
50% inhibition at 0.0022 mM and pH 5.0 or at 0.000072 mM and pH 3.0
pancreatin
-
34.2% residual activity after 1 h incubation with pancreatin at 40C
-
Pd2+
-
25% residual activity
Pepsin
-
40.8% residual activity after 1 h incubation with pepsin at 40C
-
Pepsin
-
32.4% residual activity after 1 h incubation with pepsin at 40C
-
Pepsin
-
86.7% residual activity after 1 h incubation with pepsin at 40C
-
Pepsin
-
loses 65% of the initial activity in the presence of 3000 units pepsin
-
Phenylglyoxal
-
60 nM, complete inactivation, recombinant enzyme
phenylglyoxal hydrate
-
45.3% inhibitory effect at 5 mM
phenylmethylsulfonyl fluoride
-
11.8% inhibitory effect at 10 mM
Phenylmethylsulfonylfluoride
Eupenicillium parvum
-
1 mM, 17.7% of initial activity
phosphate
-
-
phosphate
-
completely inhibited by 0.5 mM phosphate
phytate
-
free, not complexed with Ca2+, competitive inhibitor
phytate
-
substrate inhibition above 5 mM
PMSF
-
80% inhibition at 2.0 mM. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
PMSF
A4UU76
8% inhibition at 1 mM, 10% inhibition at 5 mM
Polyphosphate
-
substrate inhibition above 5 mM
SDS
-
0.0025%, complete inactivation
SDS
-
0.1%, 92% inhibition. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
SDS
-
1 mM, 0.4% of initial activity
SDS
-
complete inhibition
SDS
G4WZU3, G4WZU4
;
SO3-
-
43.1% of initial activity
sodium dodecylsulfate
Eupenicillium parvum
-
0.5 mM, 0.35% of initial activity
Triton X-100
D3HIF3
-
Urea
-
8 M, complete inactivation
Urea
-
in presence of 2 mM dithiothreitol, the inactivation and unfolding are greatly enhanced at the same concentration of denaturant. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Urea
-
8 M, 65% inhibition. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
vanadate
-
51V-NMR studies show that vanadate is incorporated in the active site. Enzyme shows higher affinity for vanadate at pH 5.0 than at pH 7.6. vanadium is covalently coordinated in the active site of the enzyme to an apical histidine and to oxygen donors. Two sites are available for coordination. Upon addition of H2O2 two peroxide-vanadate-phytase complexes are formed at pH 5.0 in the case of 3-phytase
wolframate
-
-
-
Zn2+
-
0.1-1 mM, strong inhibition
Zn2+
-
0.1 mM-1 mM, strong inhibition
Zn2+
-
67% inhibition at 5 mM, 96% inhibition at 20 mM
Zn2+
O00092
1 mM, inhibitive effect
Zn2+
-
27,34% residual activity at 5 mM
Zn2+
-
30.3% inhibitory effect at 5 mM
Zn2+
-
1 mM, 11% of initial activity
Zn2+
-
35% residual activity
Zn2+
-
0.5 mM, 96% decrease in activity
Zn2+
G4WZU3, G4WZU4
;
Zn2+
A4UU76
32% inhibition at 1 mM, 40% inhibition at 5 mM
ZnSO4
-
2.5 mM, 59% inhibition
montmorillonite
-
loss of activity upon sorption on, overview
-
additional information
-
recombinant enzyme is inhibited by antibody raised against a 20-mer internal peptide
-
additional information
-
no inhibition by myo-inositol hexasulfate up to 2 mM, no inhibition by F- up to 10 mM
-
additional information
-
not affected at concentrations up to 10 mM by: EDTA, Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+
-
additional information
-
no inhibition by EDTA and PMSF
-
additional information
-
Mg2+ and Ca2+ show no effect at 0.1 mM-1 mM
-
additional information
-
no substrate inhibition by 4-nitrophenyl phosphate up to 10 mM
-
additional information
-
not inhibited by 1 mM EDTA and 5 mM 1,10-phenanthroline
-
additional information
-
neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3-
-
additional information
D3HIF3
no inhibition by sodium azide, DTT, 2-mercaptoethanol, EDTA, toluene, glycerol, and PMSF
-
additional information
-
no or poor inhibition of isozymes RO1 and RO2 by EDTA
-
additional information
-
no inhibition by arsenite
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
0.1 mM, 59% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Ca2+
G4WZU3, G4WZU4
activates, dependent on, best at 1 mM, required for thermal stability
cetyl trimethyl ammonium bromide
-
1%, 80% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
citrate
-
0.1 mM, 45% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
EDTA
-
0.1 mM, 54% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
EDTA
-
1 mM, 113% of initial activity
EDTA
-
slight activation at 1-10 mM; slight stimulation
F-
-
200 mM, activation to 160.7% of control
iodoacetic acid
-
0.1 mM, 28% acti1vation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
L-Tartaric acid
-
0.1 mM, 34% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
oxalate
-
0.1 mM, 52% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
p-hydroxymercuribenzoate
-
0.1 mM, 22% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Phosphomycin
-
0.1 mM, 37% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
PMSF
-
0.1 mM, 63% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Sodium azide
-
0.1 mM, 37% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Triton X-100
-
enhances activity
Triton X-100
-
5%, 75% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Tween-20
-
1%, 80% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
Tween-80
-
1%, 77% activation. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
wheat bran
-
leads to sustained secretion of phytase
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.49
-
1-L-myo-inositol 1,2,3,4,5-pentakisphosphate
-
pH 5.5, 37C
0.51
-
1-L-myo-inositol 1,2,3,5,6-pentakisphosphate
-
pH 5.5, 37C
0.49
2
1-naphthyl phosphate
-
pH 5.0, 35C, phytase LP2
0.516
-
1-naphthyl phosphate
-
pH 5.0, 35C, phytase LP11
0.718
-
1-naphthyl phosphate
-
pH 5.0, 35C, phytase LP12
1.6
-
1-naphthyl phosphate
-
-
250
-
1-naphthyl phosphate
-
-
0.667
-
2-glycerophosphate
-
pH 5.0, 35C, phytase LP11
0.704
-
2-glycerophosphate
-
pH 5.0, 35C, phytase LP2
0.881
-
2-glycerophosphate
-
pH 5.0, 35C, phytase LP12
0.512
-
2-naphthyl phosphate
-
pH 5.0, 35C, phytase LP2
0.634
-
2-naphthyl phosphate
-
pH 5.0, 35C, phytase LP12
0.653
-
2-naphthyl phosphate
-
pH 5.0, 35C, phytase LP11
230
-
2-naphthyl phosphate
-
-
0.123
-
4-nitrophenyl phosphate
-
pH 5.0, 35C, phytase LP12
0.152
-
4-nitrophenyl phosphate
-
pH 5.0, 35C, phytase LP11
0.176
-
4-nitrophenyl phosphate
-
pH 5.0, 35C, phytase LP2
0.265
-
4-nitrophenyl phosphate
-
-
0.93
-
4-nitrophenyl phosphate
-
pH 5.5, 50C
1.38
-
4-nitrophenyl phosphate
-
; pH 2.5
2.2
-
4-nitrophenyl phosphate
-
pH 5.6
4.3
-
4-nitrophenyl phosphate
-
-
10.1
-
4-nitrophenyl phosphate
Eupenicillium parvum
-
pH 5.5, 50C
120
-
4-nitrophenyl phosphate
-
-
0.703
-
4-nitrophenylphosphate
-
37C, pH 4.5
0.55
-
5'-ATP
-
-
0.398
-
ADP
-
pH 5.0, 35C, phytase LP12
0.403
-
ADP
-
pH 5.0, 35C, phytase LP11
0.451
-
ADP
-
pH 5.0, 35C, phytase LP2
2.5
-
ADP
-
pH 5.5, 50C
0.315
-
AMP
-
pH 5.0, 35C, phytase LP12
0.319
-
AMP
-
pH 5.0, 35C, phytase LP2
0.365
-
AMP
-
pH 5.0, 35C, phytase LP11
0.07
-
ATP
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
0.412
-
ATP
-
pH 5.0, 35C, phytase LP12
0.531
-
ATP
-
pH 5.0, 35C, phytase LP11
0.596
-
ATP
-
pH 5.0, 35C, phytase LP2
1.7
-
ATP
-
-
1.75
-
ATP
-
pH 5.5, 50C
0.45
-
calcium myo-inositol hexakisphosphate
-
-
-
2.2
-
D-fructose 1,6-bisphosphate
-
pH 5.5, 37C
0.14
-
D-fructose 1,6-diphosphate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
0.359
-
D-fructose 1,6-diphosphate
-
pH 5.0, 35C, phytase LP2
0.731
-
D-fructose 1,6-diphosphate
-
pH 5.0, 35C, phytase LP12
1.1
-
D-fructose 1,6-diphosphate
-
-
1.9
-
D-Fructose 1-phosphate
-
pH 5.5, 37C
0.478
-
D-fructose 6-phosphate
-
pH 5.0, 35C, phytase LP11
0.531
-
D-fructose 6-phosphate
-
pH 5.0, 35C, phytase LP2
0.676
-
D-fructose 6-phosphate
-
pH 5.0, 35C, phytase LP12
0.26
-
D-glucose 1-phosphate
-
37C, pH 4.5
0.26
-
D-glucose 1-phosphate
-
pH 5.5, 37C
0.28
-
D-glucose 1-phosphate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
0.27
-
D-glucose 6-phosphate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
0.302
-
D-glucose 6-phosphate
-
pH 5.0, 35C, phytase LP2
0.401
-
D-glucose 6-phosphate
-
pH 5.0, 35C, phytase LP11
0.634
-
D-glucose 6-phosphate
-
pH 5.0, 35C, phytase LP12
1.3
-
D-glucose 6-phosphate
-
pH 5.5, 37C
2.3
-
D-glucose 6-phosphate
-
-
1.2
-
D-ribose 5-phosphate
-
-
9.8
-
D-ribose 5-phosphate
-
pH 5.5, 37C
0.17
-
di-sodium phenyl phosphate dihydrate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
-
0.445
-
diphosphate
-
pH 5.0, 35C, phytase LP11
0.691
-
diphosphate
-
pH 5.0, 35C, phytase LP12
0.812
-
diphosphate
-
pH 5.0, 35C, phytase LP2
0.44
-
dodecasodium myo-inositol hexakisphosphate
-
-
-
0.229
-
fructose 1,6-diphosphate
-
pH 5.0, 35C, phytase LP11
18
-
fructose 1,6-diphosphate
-
-
56
-
fructose 6-phosphate
-
-
20
-
glucose 6-phosphate
-
-
110
-
Glycerol 2-phosphate
-
-
0.217
-
GTP
-
pH 5.0, 35C, phytase LP2
0.398
-
GTP
-
pH 5.0, 35C, phytase LP11
0.423
-
GTP
-
pH 5.0, 35C, phytase LP12
2
-
inositol diphosphate
-
-
2
-
inositol monophosphate
-
-
2
-
inositol pentaphosphate
-
-
2
-
inositol tetraphosphate
-
-
-
0.0104
-
myo-inositol hexakisphosphate
-
pH 5.5
0.0139
-
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37C
0.0147
-
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37C
0.016
-
myo-inositol hexakisphosphate
-
-
0.021
-
myo-inositol hexakisphosphate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
0.027
-
myo-inositol hexakisphosphate
-
native fungal enzyme
0.027
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, native enzyme
0.027
-
myo-inositol hexakisphosphate
-
-
0.027
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, native enzyme
0.03
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C
0.034
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
0.038
-
myo-inositol hexakisphosphate
-
-
0.04
-
myo-inositol hexakisphosphate
-
-
0.04
-
myo-inositol hexakisphosphate
-
strain NRRL 3135
0.04
-
myo-inositol hexakisphosphate
Bacillus subtilis, Schwanniomyces castellii
-
-
0.04
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C
0.04
-
myo-inositol hexakisphosphate
O93838, P34752
strain NRRL 3135
0.04
-
myo-inositol hexakisphosphate
-
-
0.045
-
myo-inositol hexakisphosphate
-, P34752
mutant C435G, pH 2.5, 42C
0.05
-
myo-inositol hexakisphosphate
-
at pH 7.5
0.05
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, recombinant enzyme
0.05
-
myo-inositol hexakisphosphate
-
pH 7.0
0.056
-
myo-inositol hexakisphosphate
-, P34752
mutant C264G, pH 6.0, 37C
0.06
-
myo-inositol hexakisphosphate
-
at pH 6.5
0.061
-
myo-inositol hexakisphosphate
-
-
0.062
-
myo-inositol hexakisphosphate
-
high molecular weight form
0.065
-
myo-inositol hexakisphosphate
-
recombinant enzyme
0.07
-
myo-inositol hexakisphosphate
-, P34752
wild-type, pH 5.0, 58C
0.074
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
0.078
-
myo-inositol hexakisphosphate
-
pH 4.5, 55C
0.08
-
myo-inositol hexakisphosphate
-
pH 5.0, 35C, phytase LP11
0.0827
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 4.0
0.0864
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 4.0
0.0934
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P/F131L/K112R/K195R, at pH 4.0
0.1
-
myo-inositol hexakisphosphate
-
at pH 6
0.1
-
myo-inositol hexakisphosphate
-
-
0.1
-
myo-inositol hexakisphosphate
-
at pH 5.3
0.1064
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R, at 37C, in 0.2 M citrate buffer, pH 5.5
0.1076
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P, at pH 5.5
0.1083
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K, at pH 5.5
0.11
-
myo-inositol hexakisphosphate
-
-
0.114
-
myo-inositol hexakisphosphate
-
low molecular weight form
0.114
-
myo-inositol hexakisphosphate
O00092
in 200 mM sodium acetate buffer (pH 5.5), at 37C
0.1224
-
myo-inositol hexakisphosphate
-
wild type enzyme, at pH 4.0
0.124
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, recombinant enzyme
0.13
-
myo-inositol hexakisphosphate
-
-
0.13
-
myo-inositol hexakisphosphate
-
pH 5.0, 35C, phytase LP2
0.1304
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S, at 37C, in 0.2 M citrate buffer, pH 5.5
0.135
-
myo-inositol hexakisphosphate
-
immobilized enzyme
0.135
-
myo-inositol hexakisphosphate
-
-
0.145
-
myo-inositol hexakisphosphate
-, P34752
mutant C215S, pH 5.0, 37C
0.15
-
myo-inositol hexakisphosphate
-
at pH 5.5
0.15
-
myo-inositol hexakisphosphate
-
-
0.154
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/Q191R, at 37C, in 0.2 M citrate buffer, pH 5.5
0.16
-
myo-inositol hexakisphosphate
-
pH 5.0
0.1623
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/Q191R/T271R, at 37C, in 0.2 M citrate buffer, pH 5.5
0.1675
-
myo-inositol hexakisphosphate
-
wild type enzyme, at 37C, in 0.2 M citrate buffer, pH 5.5
0.17
-
myo-inositol hexakisphosphate
-
pH 5.0, 37C
0.1719
-
myo-inositol hexakisphosphate
-
wild type enzyme, at pH 5.5
0.18
-
myo-inositol hexakisphosphate
-, P34752
mutant C31G, pH 5.5, 53C
0.196
-
myo-inositol hexakisphosphate
Q6T9Z6
in 1 M sodium acetate buffer pH 5.5, at 37C
0.196
-
myo-inositol hexakisphosphate
Q7CIZ7
pH 4.5, 37C
0.2
-
myo-inositol hexakisphosphate
-
-
0.2
-
myo-inositol hexakisphosphate
-
at pH 2.5
0.2
-
myo-inositol hexakisphosphate
-
-
0.206
-
myo-inositol hexakisphosphate
B4X9S4
pH 4.5, 37C
0.24
-
myo-inositol hexakisphosphate
-
native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
0.25
-
myo-inositol hexakisphosphate
-
-
0.25
-
myo-inositol hexakisphosphate
-
-
0.252
-
myo-inositol hexakisphosphate
-
pH 5.0, 37C
0.278
-
myo-inositol hexakisphosphate
-
37C, pH 4.5
0.3
-
myo-inositol hexakisphosphate
-
-
0.3
-
myo-inositol hexakisphosphate
-
pH 5.0, 35C, phytase LP12
0.3
-
myo-inositol hexakisphosphate
-
37C, pH 3.5, enzyme expressed in Schizosaccharomyces pombe. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.33
-
myo-inositol hexakisphosphate
-
-
0.34
-
myo-inositol hexakisphosphate
Q6U677
-
0.34
-
myo-inositol hexakisphosphate
-
37C, pH 4.5
0.34
-
myo-inositol hexakisphosphate
-
pH 5.5, 37C
0.35
-
myo-inositol hexakisphosphate
-
-
0.38
-
myo-inositol hexakisphosphate
-
pH 5.5, 40C
0.3847
-
myo-inositol hexakisphosphate
-
mutant enzyme A58E/P65S/Q191R/T271R/E228K/S149P/F131L/K112R/K195R, at pH 5.5
0.39
-
myo-inositol hexakisphosphate
-
-
0.39
-
myo-inositol hexakisphosphate
A5A5J1
37C, pH 7.0
0.44
-
myo-inositol hexakisphosphate
-
strain 92
0.44
-
myo-inositol hexakisphosphate
O93838, P34752
strain 92
0.46
-
myo-inositol hexakisphosphate
-
pH 4, 37C
0.48
-
myo-inositol hexakisphosphate
-
strain IIIAn/8
0.5
-
myo-inositol hexakisphosphate
-
Bacillus subtilis var. natto
0.52
-
myo-inositol hexakisphosphate
Q84B22
pH 7.5, 55C
0.532
-
myo-inositol hexakisphosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37C
0.532
-
myo-inositol hexakisphosphate
-
recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
0.55
-
myo-inositol hexakisphosphate
-
-
0.58
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
sodium salt, pH 5.5, 50C
0.59
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
calcium salt, pH 5.5, 50C
0.606
-
myo-inositol hexakisphosphate
-
pH 2.5, 55C. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.63
-
myo-inositol hexakisphosphate
-
-
0.7
-
myo-inositol hexakisphosphate
-
37C, pH 3.5, enzyme expressed in Pichia pastoris (pPICZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.8
-
myo-inositol hexakisphosphate
-
37C, pH 3.5, enzyme expressed in Pichia pastoris (pGAPZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
0.91
-
myo-inositol hexakisphosphate
-
-
0.98
-
myo-inositol hexakisphosphate
-
-
1
-
myo-inositol hexakisphosphate
-
immobilized enzyme
1.2
-
myo-inositol hexakisphosphate
-
37C, pH 3.5, enzyme expressed in Saccharomyces cerevisiae. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
1.45
-
myo-inositol hexakisphosphate
-
5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60C
1.5
-
myo-inositol hexakisphosphate
-
37C, pH 5.5, enzyme expressed in Schizosaccharomyces pombe. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
1.8
-
myo-inositol hexakisphosphate
-
37C, pH 5.5, enzyme expressed in Pichia pastoris (pGAPZalphaA) or Pichia pastoris (pPICZalphaA). The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
2
-
myo-inositol hexakisphosphate
-
-
2.1
-
myo-inositol hexakisphosphate
-
37C, pH 5.5, enzyme expressed in Saccharomyces cerevisiae. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
2.1
-
myo-inositol hexakisphosphate
-
-
2.8
-
myo-inositol hexakisphosphate
-
-
2.84
-
myo-inositol hexakisphosphate
-
; pH 5.5
114.8
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
potassium salt, pH 5.5, 50C
0.14
-
myo-inositol hexakisphosphate disodium salt
B3VPB2
pH 5.5, 50C
0.15
-
myo-inositol hexakisphosphate disodium salt
B3VPB3
pH 5.5, 50C
0.08
-
myo-inositol hexakisphosphate sodium salt
B3VPB2
pH 5.5, 50C
0.16
-
myo-inositol hexakisphosphate sodium salt
B3VPB3
pH 5.5, 50C
0.035
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme a1, pH 5.0, 36C
0.036
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme a, pH 5.0, 36C
0.045
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 2.5, 42C, recombinant mutant C435G
0.045
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme b1, pH 5.0, 36C
0.046
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme b2, pH 5.0, 36C
0.048
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL6
recombinant isozyme b, pH 5.0, 36C
0.056
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 6.0, 37C, recombinant mutant C264G
0.07
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 58C, recombinant wild-type enzyme
0.13
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
isozyme RO2, pH 4.5, 40C
0.145
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 37C, recombinant mutant C215S
0.18
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.5, 53C, recombinant mutant C31G
0.19
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant wild-type enzyme, pH 4.5, 37C
0.191
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase 168PhyA, pH 7.0, 55C
0.2
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D3HIF3
pH 4.0, 60C
0.24
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase 168PhyA, pH 7.0, 55C
0.42
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
pH 7.0, 37C
0.45
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79L, pH 4.5, 37C
0.5
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
dual domain phytase PhyH, pH 7.0, 35C
0.52
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
pH 5.5, 50C
0.55
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79S, pH 4.5, 37C
0.69
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79G, pH 4.5, 37C
1.086
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase PhyP, pH 7.0, 37C
1.28
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase PhyP, pH 7.0, 37C
1.432
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DII, pH 7.0, 35C
1.6
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
isozyme RO1, pH 4.5, 40C
1.98
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37C
3.024
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37C
8.442
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37C
78
-
Na2H2pyrophosphate
-
-
3.8
-
naphthyl 1-phosphate
-
pH 5.5, 50C
-
0.74
-
O-phospho-L-serine
-
pH 5.0, 35C, phytase LP12
0.8
11
O-phospho-L-serine
-
pH 5.0, 35C, phytase LP2
0.891
-
O-phospho-L-serine
-
pH 5.0, 35C, phytase LP11
0.52
-
p-nitrophenyl phosphate
-
at 62C, in 50 mM sodium acetate buffer (pH 5)
2.27
-
p-nitrophenyl phosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37C
12.4
-
p-nitrophenyl phosphate
-
37C, pH 4.5
18.16
-
p-nitrophenyl phosphate
Q6T9Z6
in 1 M sodium acetate buffer pH 5.5, at 37C
2.4
-
phosphate
-
-
0.801
-
phosphoenolpyruvate
-
37C, pH 4.5
0.087
0.324
phytate
-
pH 2.5, 55C
0.138
-
phytate
-
wild-type enzyme
0.14
-
phytate
G4WZU3, G4WZU4
pH 5.0, 37C
0.25
0.425
phytate
Q6RK08
pH 4.0, 55C
0.67
-
phytate
G4WZU3, G4WZU4
pH 7.5, 37C
0.747
-
phytate
-
mutant enzyme E227A
0.914
-
phytate
-
mutant enzyme D55A
0.934
-
phytate
-
mutant enzyme Y159H
0.967
-
phytate
-
mutant enzyme K76E
2.505
-
phytate
-
mutant enzyme R122E
3.046
-
phytate
-
mutant enzyme R122K
3.542
-
phytate
-
mutant enzyme D258A
8.661
-
phytate
-
mutant enzyme Y159A
11.99
-
phytate
-
mutant enzyme K76R
3.3
-
Polyphosphate
-
-
0.776
-
pyridoxal phosphate
-
pH 5.0, 35C, phytase LP11
0.876
-
pyridoxal phosphate
-
pH 5.0, 35C, phytase LP12
0.915
-
pyridoxal phosphate
-
pH 5.0, 35C, phytase LP2
2
-
inositol triphosphate
-
-
additional information
-
additional information
O31097
typical Michaelis-Menten kinetics
-
additional information
-
additional information
C4PKL2, C4PKL4
substrate specificity and kinetics of recombinant isozymes, overview; substrate specificity and kinetics of recombinant isozymes, overview
-
additional information
-
additional information
C4PKK7, C4PKK9
substrate specificity and kinetics of recombinant isozymes, overview; substrate specificity and kinetics of recombinant isozymes, overview
-
additional information
-
additional information
C4PKL6
substrate specificity and kinetics of recombinant isozymes, overview
-
additional information
-
additional information
-
typical Michaelis-Menten kinetics
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
16
-
1-L-myo-inositol 1,2,3,4,5-pentakisphosphate
-
pH 5.5, 37C
16
-
1-L-myo-inositol 1,2,3,5,6-pentakisphosphate
-
pH 5.5, 37C
2
-
1-naphthyl phosphate
-
-
6
-
2-naphthyl phosphate
-
-
11.8
-
4-nitrophenyl phosphate
Eupenicillium parvum
-
pH 5.5, 50C
20
-
4-nitrophenyl phosphate
-
-
99
-
4-nitrophenyl phosphate
-
-
104
-
4-nitrophenyl phosphate
-
-
357500
-
4-nitrophenyl phosphate
-
pH 2.5
358000
-
4-nitrophenyl phosphate
-
pH 2.5
161.9
-
4-nitrophenylphosphate
-
37C, pH 4.5
1.1
-
D-fructose 1,6-diphosphate
-
pH 5.5, 37C
1.6
-
D-Fructose 1-phosphate
-
pH 5.5, 37C
101
-
D-glucose 1-phosphate
-
37C, pH 4.5
101
-
D-glucose 1-phosphate
-
pH 5.5, 37C
2.9
-
D-glucose 6-phosphate
-
pH 5.5, 37C
1.1
-
D-ribose 5-phosphate
-
pH 5.5, 37C
11
-
diphosphate
-
-
10
-
fructose 1,6-diphosphate
-
-
6
-
fructose 6-phosphate
-
-
2
-
glucose 6-phosphate
-
-
0.93
-
myo-inositol hexakisphosphate
-
5.0 mM myo-inositol hexakisphosphate in 0.2 M sodium acetate, pH 5.0, at 60C
3
6
myo-inositol hexakisphosphate
-, P34752
mutant C264G, pH 6.0, 37C
8.85
-
myo-inositol hexakisphosphate
-
37C, pH 4.5
21
-
myo-inositol hexakisphosphate
-
37C, pH 4.5
21
-
myo-inositol hexakisphosphate
-
pH 5.5, 37C
26.6
-
myo-inositol hexakisphosphate
-
pH 7.0
46
-
myo-inositol hexakisphosphate
-
; pH 5.0, 58C
51
-
myo-inositol hexakisphosphate
-
pH 5.5
51.2
-
myo-inositol hexakisphosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
51.2
-
myo-inositol hexakisphosphate
-
recombinant enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
59.2
-
myo-inositol hexakisphosphate
-
native enzyme, in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
62
-
myo-inositol hexakisphosphate
-, P34752
mutant C215S, pH 5.0, 37C
90
-
myo-inositol hexakisphosphate
-, P34752
mutant C435G, pH 2.5, 42C
102
-
myo-inositol hexakisphosphate
O00092
-
103
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
sodium salt, pH 5.5, 50C
114
-
myo-inositol hexakisphosphate
-
-
117
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
calcium salt, pH 5.5, 50C
134
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
potassium salt, pH 5.5, 50C
170
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
180
-
myo-inositol hexakisphosphate
-
-
220
-
myo-inositol hexakisphosphate
-
-
234
-
myo-inositol hexakisphosphate
-, P34752
mutant C31G, pH 5.5, 53C
300
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, recombinant enzyme
348
-
myo-inositol hexakisphosphate
-
native fungal enzyme
348
-
myo-inositol hexakisphosphate
-
; pH 5.0, 58C
348
-
myo-inositol hexakisphosphate
-
pH 5.0, 58C, native enzyme
350
-
myo-inositol hexakisphosphate
-
-
350
-
myo-inositol hexakisphosphate
-, P34752
wild-type, pH 5.0, 58C
360
-
myo-inositol hexakisphosphate
-
recombinant enzyme
398
-
myo-inositol hexakisphosphate
Q6T9Z6
in 1 M sodium acetate buffer pH 5.5, at 37C
840
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
908
-
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37C
950
-
myo-inositol hexakisphosphate
-
pH 5.0, 37C
2056
-
myo-inositol hexakisphosphate
-
in 50 mM NaOAc (pH 5.0) or 100 mM Tris-HCl buffer (pH 8.0), at 37C
2283
-
myo-inositol hexakisphosphate
Q7CIZ7
pH 4.5, 37C
2531
-
myo-inositol hexakisphosphate
B4X9S4
pH 4.5, 37C
300000
-
myo-inositol hexakisphosphate
-
pH 2.5, 55C. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
411700
-
myo-inositol hexakisphosphate
-
pH 5.5
412000
-
myo-inositol hexakisphosphate
-
pH 5.5
231
-
myo-inositol hexakisphosphate disodium salt
B3VPB2
pH 5.5, 50C
281
-
myo-inositol hexakisphosphate disodium salt
B3VPB3
pH 5.5, 50C
253
-
myo-inositol hexakisphosphate sodium salt
B3VPB3
pH 5.5, 50C
279
-
myo-inositol hexakisphosphate sodium salt
B3VPB2
pH 5.5, 50C
3
6
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 6.0, 37C, recombinant mutant C264G
4.17
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DII, pH 7.0, 35C
5.92
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase 168PhyA, pH 7.0, 55C
12.28
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase 168PhyA, pH 7.0, 55C
27.72
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
dual domain phytase PhyH, pH 7.0, 35C
44.94
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase PhyP, pH 7.0, 37C
59.83
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37C
62
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 37C, recombinant mutant C215S
83.67
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37C
90
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 2.5, 42C, recombinant mutant C435G
94.4
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase PhyP, pH 7.0, 37C
161
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37C
234
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.5, 53C, recombinant mutant C31G
248
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL6
recombinant isozyme b, pH 5.0, 36C
253
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme b2, pH 5.0, 36C
260
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme a, pH 5.0, 36C
270
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme b1, pH 5.0, 36C
279
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme a1, pH 5.0, 36C
350
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 58C, recombinant wild-type enzyme
370
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
Q84CN9
pH not specified in the publication, temperature not specified in the publication
1.9
-
p-nitrophenyl phosphate
Q6T9Z6
in 1 M sodium acetate buffer pH 5.5, at 37C
8.2
-
p-nitrophenyl phosphate
-
37C, pH 4.5
44.2
-
p-nitrophenyl phosphate
-
in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37C
198
-
phosphoenolpyruvate
-
37C, pH 4.5
0.82
-
phytate
-
mutant enzyme K76R
23.46
-
phytate
G4WZU3, G4WZU4
pH 7.5, 37C
27
-
phytate
-
mutant enzyme Y159H
112.7
-
phytate
G4WZU3, G4WZU4
pH 5.0, 37C
117
-
phytate
-
mutant enzyme D55A
1260
-
phytate
-
pH 2.5, 55C
2348
4470
phytate
Q6RK08
pH 4.0, 55C
5530
-
phytate
-
mutant enzyme E227A
5532
-
phytate
-
mutant enzyme E227A
11800
-
phytate
-
mutant enzyme R122K
11810
-
phytate
-
mutant enzyme R122K
12400
-
phytate
-
mutant enzyme D258A
12430
-
phytate
-
mutant enzyme D258A
29060
-
phytate
-
mutant enzyme R122E
29100
-
phytate
-
mutant enzyme R122E
59550
-
phytate
-
wild-type enzyme
59600
-
phytate
-
wild-type enzyme
4
-
Glycerol 2-phosphate
-
-
additional information
-
additional information
-
turnover number of immobilized enzyme
-
additional information
-
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
88.8
-
4-nitrophenyl phosphate
Eupenicillium parvum
-
pH 5.5, 50C
4742
3.77
-
myo-inositol hexakisphosphate
-
pH 5.0, 37C
13268
11.6
-
myo-inositol hexakisphosphate
Q7CIZ7
pH 4.5, 37C
13268
12.3
-
myo-inositol hexakisphosphate
B4X9S4
pH 4.5, 37C
13268
31.8
-
myo-inositol hexakisphosphate
-
37C, pH 4.5
13268
173.7
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
potassium salt, pH 5.5, 50C
13268
177.9
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
sodium salt, pH 5.5, 50C
13268
198.9
-
myo-inositol hexakisphosphate
Eupenicillium parvum
-
calcium salt, pH 5.5, 50C
13268
430
-
myo-inositol hexakisphosphate
-, P34752
mutant C215S, pH 5.0, 37C
13268
720
-
myo-inositol hexakisphosphate
-, P34752
mutant C264G, pH 6.0, 37C
13268
1300
-
myo-inositol hexakisphosphate
-, P34752
mutant C31G, pH 5.5, 53C
13268
2000
-
myo-inositol hexakisphosphate
-, P34752
mutant C435G, pH 2.5, 42C
13268
5000
-
myo-inositol hexakisphosphate
-, P34752
wild-type, pH 5.0, 58C
13268
5000
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
13268
11400
-
myo-inositol hexakisphosphate
-
pH 3.5, 37C
13268
1651
-
myo-inositol hexakisphosphate disodium salt
B3VPB2
pH 5.5, 50C
302383
1874
-
myo-inositol hexakisphosphate disodium salt
B3VPB3
pH 5.5, 50C
302383
1584
-
myo-inositol hexakisphosphate sodium salt
B3VPB3
pH 5.5, 50C
302382
3491
-
myo-inositol hexakisphosphate sodium salt
B3VPB2
pH 5.5, 50C
302382
0.00043
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 37C, recombinant mutant C215S
136191
0.00072
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 6.0, 37C, recombinant mutant C264G
136191
0.0013
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.5, 53C, recombinant mutant C31G
136191
0.002
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 2.5, 42C, recombinant mutant C435G
136191
0.005
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-, P34752
pH 5.0, 58C, recombinant wild-type enzyme
136191
2.9
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DII, pH 7.0, 35C
136191
19.17
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37C
136191
27.83
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37C
136191
30
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant wild-type enzyme, pH 4.5, 37C
136191
30.5
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37C
136191
35
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase PhyP, pH 7.0, 37C
136191
54
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
dual domain phytase PhyH, pH 7.0, 35C
136191
87
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase PhyP, pH 7.0, 37C
136191
134
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79L, pH 4.5, 37C
136191
250
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
beta-propeller phytase 168PhyA, pH 7.0, 55C
136191
517
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL6
recombinant isozyme b, pH 5.0, 36C
136191
550
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme b2, pH 5.0, 36C
136191
600
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme b1, pH 5.0, 36C
136191
640
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
-
phytase domain Phy-DI fused to beta-propeller phytase 168PhyA, pH 7.0, 55C
136191
682
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79S, pH 4.5, 37C
136191
722
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKL2, C4PKL4
recombinant isozyme a, pH 5.0, 36C
136191
796
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
C4PKK7, C4PKK9
recombinant isozyme a1, pH 5.0, 36C
136191
4605
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
D9D7K9
recombinant enzyme mutant R79G, pH 4.5, 37C
136191
7900
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate
Q84CN9
pH not specified in the publication, temperature not specified in the publication
136191
248
-
phosphoenolpyruvate
-
37C, pH 4.5
15572
0.0039
-
phytate
-
pH 2.5, 55C
79343
0.0093
0.01
phytate
Q6RK08
pH 4.0, 55C
79343
232
-
4-nitrophenylphosphate
-
37C, pH 4.5
23782
additional information
-
additional information
-
the tandemly repeated domains of a beta-propeller phytase act synergistically to increase catalytic efficiency, fusion of domain PhyH-DI with PhyP or 168PhyA significantly enhanced their catalytic efficiencies
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.97
-
Ca2+
-
free, not complexed with phytate, competitive inhibitor
0.004
-
metavanadate
Q6RK08
pH 4.0, 55C, 0.010 mM metavanadate
-
0.00008
-
myo-inositol hexasulfate
-
pH 5.0, 58C, recombinant enzyme
0.00064
-
myo-inositol hexasulfate
-
ph 5.0, 58C
0.00156
-
myo-inositol hexasulfate
-
pH 5.0, 58C, recombinant enzyme
0.0046
-
myo-inositol hexasulfate
-
pH 5.0, 58C, native enzyme
0.0046
-
myo-inositol-1,2,3,4,5,6-hexakissulfate
-
pH 5.0-5.5, temperature not specified in the publication
-
0.0002
-
orthovanadate
Q6RK08
pH 4.0, 55C, 0.0005 mM orthovanadate
0.0057
-
orthovanadate
-
pH 2.5, 55C, 0.010 mM orthovanadate
2.3
-
phytate
-
free, not complexed with Ca2+, competitive inhibitor
0.1
-
vanadate
-
-
0.0056
-
metavanadate
-
pH 2.5, 55C, 0.015 mM metavanadate
-
additional information
-
additional information
-
vanadate inhibition kinetics
-
additional information
-
additional information
Q6RK08
vanadate inhibition kinetics
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.23
-
G4WZU3, G4WZU4
recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37C, in a strain grown under phosphate limitation in the presence of phytate
0.58
-
G4WZU3, G4WZU4
recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37C, in a strain grown under phosphate limitation in the presence of phytate
0.744
-
-
root exsudate, at 37C
1.3
-
-
recombinant mutant E227S, pH 7.0, 70C
1.4
-
O31097
purified recombinant deglycosylated enzyme, pH 7.5, 37C
1.82
-
-
functional domain Phyd-DII, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 35C
3.28
-
D9D7K9
purified recombinant wild-type enzyme, pH 4.5, 37C
4.43
-
-
dual domain PhyH, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 35C
5.7
-
-
recombinant mutant K179R, pH 7.0, 70C
7.3
-
-
recombinant mutant K77R, pH 7.0, 70C
7.5
-
-
recombinant mutant K177R/K179R, pH 7.0, 70C
8.82
-
-
purified recombinant His-tagged wild-type enzyme, pH 5.0, 37C
8.9
-
O31097
recombinant enzyme secreted from heterologously expressing Pichia pastoris, pH 5.5, 37C
9.5
-
G4WZU3, G4WZU4
purified recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37C, substrate phytate
10
-
-
37C, pH 4.5
11
-
O31097
purified recombinant enzyme, pH 7.5, 37C
13.01
-
-
beta-propeller phytase 168PhyA, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 55C
14.87
-
-
incomplete domain Phyd-DI fused to beta-propeller phytase 168PhyA, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 55C
16
-
A5A5J1
37C, pH 7.0
18.5
-
-
recombinant mutant P257R, pH 7.0, 70C
20.3
-
D9D7K9
recombinant enzyme mutant R79L, pH 4.5, 37C
21.61
-
-
beta-propeller phytase PhyP, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 37C
22.1
-
-
purified recombinant His-tagged mutant K41E, pH 5.0, 37C
25
-
Q84B22
pH 7.5, 55C
27.28
-
-
purified recombinant His-tagged mutant E121F, pH 5.0, 37C
28.7
-
A4UU76
purifed recombinant enzyme, pH 7.5, 60C
29.82
-
-
incomplete domain Phyd-DI fused to beta-propeller phytase PhyP, myo-inositol-1,2,3,4,5,6-hexakisphosphate as substrate, pH 7.0, 37C
31.2
-
-
recombinant mutant E180N, pH 7.0, 70C
32.2
-
-
recombinant wild-type enzyme, pH 7.0, 70C
32.76
-
A2TEY6, -
wild-type enzyme, pH 5.5-6.0, 37C
33
-
-
purified isozyme RO2, pH 4.5, 40C
35
-
Janthinobacterium sp.
G0XZM1
purified recombinant domain C towards phytate, pH 8.5, 45C
35.7
-
-
recombinant mutant S283R, pH 7.0, 70C
36
-
-
-
37.8
-
-
recombinant mutant E229V, pH 7.0, 70C
53.13
-
-
recombinant enzyme, at 37C
54
-
-
purified isozyme RO1, pH 4.5, 40C
62.5
-
-
-
79.3
-
-
purified enzyme, pH 7.0, 37C
86.1
-
-
purified native enzyme, pH 5.5, 50C, substrate phytate
89.4
-
D9D7K9
recombinant enzyme mutant R79S, pH 4.5, 37C
101
-
-
crude extract, recombinant enzyme, at 37C
114.6
-
-
supernantant, at 60C
133.3
-
A2TEY6, -
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 37C
136.6
-
A2TEY6, -
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 37C
144
-
G4WZU3, G4WZU4
purified recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37C, substrate phytate
145.9
-
A2TEY6, -
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 50C
150
-
-
37C, pH 2.5
150.1
-
-
purified native enzyme, pH 2.5, 37C
150.4
-
A2TEY6, -
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 50C
157.4
-
-
purified extract, at pH 4.0
179.5
-
-
recombinant enzyme in potato leaves
182
-
-
after 1.8fold purification, recombinant enzyme, at 37C
361.7
-
-
after 486fold purification, at 37C
428
-
-
pH 2.5, 37C
498
-
-
phytase LP2
503
-
Q6T9Z6
recombinant enzyme, at 37C
539
-
-
phytase LP11
607
-
-
phytase LP12
792
-
-
pH 5.0, 37C
823.3
-
-
after 7.2fold purification, at 60C
870
-
-
pH 6.5, 37C
1072
-
-
pH 5.0, 37C
2344
-
Q7CIZ7
pH 4.5, 37C
2456
-
B4X9S4
pH 4.5, 37C
2615
-
D9D7K9
recombinant enzyme mutant R79G, pH 4.5, 37C
2656
-
-
pH 4.5, 55C
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
692.0 nKat/ml, recombinant enzyme
additional information
-
-
-
additional information
-
-
32000 OD355/mg
additional information
-
-
10 phytase units per gram dry matter at pH 4.5 and 37C for recombinant enzyme expressed in Chlamydomonas rheinhardtii
additional information
-
Janthinobacterium sp.
G0XZM1
no activity of purified recombinant domain N
additional information
-
-
phytase activity and phytic acid and total protein contents in grains of cultivated and tibetan wild barleys, overview
additional information
-
D3HIF3
activity during recycling of immobilized permeabilized cells, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
-
-
and a second optimum at pH 5.5
2
-
-
optimum 1
2.2
-
-
and a second optimum which is higher at pH 5.0-5.5
2.2
-
O93838, P34752
strain NRRL 3135, an a second optimum at pH 5.0-5.5
2.5
5
-
hydrolysis of myo-inositol hexakisphosphate
2.5
-
-
and a second optimum which is higher at pH 5.0
2.5
-
-
and a second higher pH optimum at pH 5.5
2.5
-
-
optima at pH 2.5 and 5.5
2.5
-
Q6T9Z6
recombinant enzyme
2.5
-
-, P34752
mutant C435G; recombinant mutant C435G
2.7
-
-
and a second optimum at pH 5.0
3
4.5
-
hydrolysis of 4-nitrophenyl phosphate
3
-
-
at 58C, and a second optimum at pH 5.5
3
-
Penicillium caseoicolum
-
-
3
-
Q6U677
substrate: D-ribose 5-phosphate
3
-
-
and 5.5
3
-
-
optimum I for both isozymes RO1 and RO2
3.5
4.5
-
hydrolysis of 4-nitrophenyl phosphate
3.5
-
-
recombinant enzyme. The enzyme may be a 3-phytase, EC 3.1.3.8, or a 6-phytase, EC 3.1.3.26. The product of the hydrolysis of myo-inositol hexakisphosphate i.e. myo-inositol 1,2,3,4,5-pentakisphosphate or myo-inositol 1,3,4,5,6-pentakisphosphate has not been identified
3.5
-
Q6U677
substrate: D-glucose 1-phosphate, D-glucose 6-phosphate and D-fructose 1-phosphate