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Literature summary for 3.1.3.8 extracted from

  • Fu, D.; Huang, H.; Luo, H.; Wang, Y.; Yang, P.; Meng, K.; Bai, Y.; Wu, N.; Yao, B.
    A highly pH-stable phytase from Yersinia kristeensenii: Cloning, expression, and characterization (2008), Enzyme Microb. Technol., 42, 499-505.
No PubMed abstract available
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Yersinia kristensenii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 61% residual activity Yersinia kristensenii
Fe3+ complete inhibition Yersinia kristensenii
Hg2+ 10% residual activity Yersinia kristensenii
Pd2+ 25% residual activity Yersinia kristensenii
SDS complete inhibition Yersinia kristensenii
Zn2+ 35% residual activity Yersinia kristensenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
 myo-inositol hexakisphosphate pH 4.5, 55°C Yersinia kristensenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45900
-
 x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii
48000
-
 x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii

Organism

Organism UniProt Comment Textmining
Yersinia kristensenii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains a putative N-glycosylation site at residue R198, molecular mass decreases after treatment with Endo H Yersinia kristensenii
proteolytic modification sequence contains a signal peptide of 24 amino acids Yersinia kristensenii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2656
-
 pH 4.5, 55°C Yersinia kristensenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Yersinia kristensenii 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
soybean meal + H2O
-
 Yersinia kristensenii ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45900, calculated for mature protein, x * 48000, SDS-PAGE Yersinia kristensenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Yersinia kristensenii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 10 min, 46% residual activity Yersinia kristensenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Yersinia kristensenii

pH Range

pH Minimum pH Maximum Comment Organism
2 6.5
-
 Yersinia kristensenii

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 11 37°C, 3 h, more than 90% of initial activity Yersinia kristensenii

pI Value

Organism Comment pI Value Maximum pI Value
Yersinia kristensenii calculated
-
 6.2