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Literature summary for 3.1.3.8 extracted from

  • Mitchell, D.B.; Vogel, K.; Weimann, B.J.; Pasamontes, L.; van Loon, A.P.G.M.
    The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila (1997), Microbiology, 143, 245-252.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Aspergillus niger
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-
-
Aspergillus terreus
-
-
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Aspergillus terreus 9A1
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-
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Thermothelomyces heterothallicus
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The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
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Aspergillus niger 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
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Aspergillus terreus 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Aspergillus terreus 9A1 4-nitrophenol + phosphate
-
?
myo-inositol hexakisphosphate + H2O
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Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
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Aspergillus terreus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
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Thermothelomyces heterothallicus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
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Aspergillus terreus 9A1 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 5 hydrolysis of myo-inositol hexakisphosphate Aspergillus niger
3 4.5 hydrolysis of 4-nitrophenyl phosphate Aspergillus terreus
3.5 4.5 hydrolysis of 4-nitrophenyl phosphate Thermothelomyces heterothallicus
5.5
-
-
Aspergillus niger
5.5
-
hydrolysis of myo-inositol hexakisphosphate Aspergillus terreus
5.5
-
hydrolysis of myo-inositol hexakisphosphate Thermothelomyces heterothallicus

pH Range

pH Minimum pH Maximum Comment Organism
2 5.7 pH 2.0: about 10% of maximal activity, pH 5.7: about 40% of maximal activity, hydrolysis of myo-inositol hexakisphosphate Aspergillus niger
4.5 7 pH 4.5: about 70% of maximal activity, pH 7.0: about 45% of maximal activity, hydrolysis of myo-inositol hexakisphosphate Thermothelomyces heterothallicus
4.5 6.5 pH 4.5: about 40% of maximal activity, pH 6.5: about 70% of maximal activity, hydrolysis of myo-inositol hexakisphosphate Aspergillus terreus