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Literature summary for 3.1.3.8 extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.
    Vanadate inhibition of fungal PhyA and bacterial AppA2 histidine acid phosphatases (2011), J. Agric. Food Chem., 59, 1739-1743.
    View publication on PubMed
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
metavanadate 50% inhibition at 0.0011 and pH 2.5, or at 0.0053 mM and pH 5.0 Aspergillus niger
metavanadate 50% inhibition at 0.00075 mM and pH 5.0 or at 0.00090 mM and pH 3.0 Escherichia coli
orthovanadate 50% inhibition at 0.0004 and pH 2.5, or at 0.040 mM and pH 5.0 Aspergillus niger
orthovanadate 50% inhibition at 0.0022 mM and pH 5.0 or at 0.000072 mM and pH 3.0 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087 0.324 phytate pH 2.5, 55°C Aspergillus niger
0.25 0.425 phytate pH 4.0, 55°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phytate + H2O Aspergillus niger
-
 ? + phosphate
-
 ?
phytate + H2O Escherichia coli
-
 ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 gene phyA
-
Escherichia coli Q6RK08 gene AppA2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phytate + H2O
-
 Aspergillus niger ? + phosphate
-
 ?
phytate + H2O
-
 Escherichia coli ? + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
AppA2
-
 Escherichia coli
histidine acid phosphatase
-
 Aspergillus niger
histidine acid phosphatase
-
 Escherichia coli
PhyA
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Aspergillus niger
55
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1260
-
 phytate pH 2.5, 55°C Aspergillus niger
2348 4470 phytate pH 4.0, 55°C Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5
-
 Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information vanadate inhibition kinetics Aspergillus niger
additional information
-
 additional information vanadate inhibition kinetics Escherichia coli
0.0002
-
 orthovanadate pH 4.0, 55°C, 0.0005 mM orthovanadate Escherichia coli
0.004
-
 metavanadate pH 4.0, 55°C, 0.010 mM metavanadate Escherichia coli
0.0056
-
 metavanadate pH 2.5, 55°C, 0.015 mM metavanadate Aspergillus niger
0.0057
-
 orthovanadate pH 2.5, 55°C, 0.010 mM orthovanadate Aspergillus niger

General Information

General Information Comment Organism
additional information the enzyme can also act as a vanadate haloperoxidase, EC 1.11.1.10 Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0039
-
 phytate pH 2.5, 55°C Aspergillus niger
0.0093 0.01 phytate pH 4.0, 55°C Escherichia coli