Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.8 extracted from

  • Kerovuo, J.; Lauraeus, M.; Nurminen, P.; Kalkkinen, N.; Apajalahti, J.
    Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis (1998), Appl. Environ. Microbiol., 64, 2079-2085.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus subtilis

General Stability

General Stability Organism
Ca2+ is required for stability Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis VTT E-68013
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ?
-
?
ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ?
-
?
ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ?
-
?
ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ?
-
?
myo-inositol hexakisphosphate + H2O
-
Bacillus subtilis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O
-
Bacillus subtilis VTT E-68013 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
at 55°C Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 35% of maximal activity, pH 7.5: about 95% of maximal activity Bacillus subtilis