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Literature summary for 3.1.3.8 extracted from

  • Ariza, A.; Moroz, O.V.; Blagova, E.V.; Turkenburg, J.P.; Waterman, J.; Roberts, S.M.; Vind, J.; Sj?holm, C.; Lassen, S.F.; De Maria, L.; Glitsoe, V.; Skov, L.K.; Wilson, K.S.
    Degradation of phytate by the 6-phytase from Hafnia alvei: a combined structural and solution study (2013), PLoS ONE, 8, e65062.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Hafnia alvei
-
Yersinia kristensenii

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-enzyme, to 1.9 A resolution, and in complex with substrate analogue myo-inositiol hexakissulfate, to 1.59 A resolution. The substrate analogue is coordinated by residues Arg18, His19, Arg22, Thr25, Lys26, His128, Thr219, His306, Asp307 and a number of water molecules. The Nepsilon2 atom of the catalytic nucleophile, His19, lies 3.2 A from S6 of the analogue and is in-line with the S-O bond from the inositol ring. The OD1 atom of Asp307, the proton donor, forms an H-bond with the oxygen at position 6 of the inositol ring Hafnia alvei
in complex with phosphate, to 1.9 A resolution Yersinia kristensenii

Protein Variants

Protein Variants Comment Organism
T308A 5% of the specific activity of wild-type Hafnia alvei

Organism

Organism UniProt Comment Textmining
Hafnia alvei H9TUK5
-
-
Yersinia kristensenii H9TUK6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Hafnia alvei
recombinant protein Yersinia kristensenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Hafnia alvei ?
-
?
additional information enzyme is a 6-phytase, EC 3.1.3.26. Major degradation product is DL-inositol 1,2,3,4,5-pentaphosphate Yersinia kristensenii ?
-
?
myo-inositol hexakisphosphate + H2O
-
Hafnia alvei DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ?
myo-inositol hexakisphosphate + H2O
-
Yersinia kristensenii DL-inositol 1,2,4,5,6-pentaphosphate + phosphate 10-14% of product ?

Synonyms

Synonyms Comment Organism
PhyA
-
Hafnia alvei
PhyA
-
Yersinia kristensenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Yersinia kristensenii
65
-
-
Hafnia alvei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
melting temperature Yersinia kristensenii
70
-
melting temperature Hafnia alvei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
Hafnia alvei
4 4.5
-
Yersinia kristensenii