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Literature summary for 3.1.3.8 extracted from

  • Spier, M.; Fendrich, R.; Almeida, P.; Noseda, M.; Greiner, R.; Konietzny, U.; Woiciechowski, A.; Soccol, V.; Soccol, C.
    Phytase produced on citric byproducts: purification and characterization (2011), World J. Microbiol. Biotechnol., 27, 267-274.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information typical Michaelis-Menten kinetics Aspergillus niger
0.52
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate pH 5.5, 50°C Aspergillus niger
0.93
-
4-nitrophenyl phosphate pH 5.5, 50°C Aspergillus niger
1.75
-
ATP pH 5.5, 50°C Aspergillus niger
2.5
-
ADP pH 5.5, 50°C Aspergillus niger
3.8
-
1-naphthyl phosphate pH 5.5, 50°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
x * 108000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Aspergillus niger
-
? + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
isolated from soil samples collected in Northeast Parana State in Brazil
-
Aspergillus niger FS3
-
isolated from soil samples collected in Northeast Parana State in Brazil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 10.1fold by cationic exchange chromatography, anionic exchange chromatography, and chromatofocusing Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
additional information growth on citric pulp fermentation Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
86.1
-
purified native enzyme, pH 5.5, 50°C, substrate phytate Aspergillus niger

Storage Stability

Storage Stability Organism
4°C, purified native enzyme, after 30 days, 88.9% activity are remaining, after 60 days the remaining activity is 84.25% of initial activity, and after 180 days 57.5% Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
Aspergillus niger 1-naphthol + phosphate
-
?
1-naphthyl phosphate + H2O
-
Aspergillus niger FS3 1-naphthol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Aspergillus niger 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Aspergillus niger FS3 4-nitrophenol + phosphate
-
?
ADP + H2O
-
Aspergillus niger AMP + phosphate
-
?
ADP + H2O
-
Aspergillus niger FS3 AMP + phosphate
-
?
ATP + H2O
-
Aspergillus niger ADP + phosphate
-
?
ATP + H2O
-
Aspergillus niger FS3 ADP + phosphate
-
?
additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger ?
-
?
additional information substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview Aspergillus niger FS3 ?
-
?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Aspergillus niger ? + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 108000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
acid phytase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified enzyme, residual activity of 86.0%, 68.4%, and 48.2% after 10, 20 or 40 min, respectively Aspergillus niger
70
-
purified enzyme, 40 min Aspergillus niger
80
-
purified enzyme, 30 min, 30.5% activity remaining Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Aspergillus niger