KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | typical Michaelis-Menten kinetics | Aspergillus niger | |
0.52 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | pH 5.5, 50°C | Aspergillus niger | |
0.93 | - |
4-nitrophenyl phosphate | pH 5.5, 50°C | Aspergillus niger | |
1.75 | - |
ATP | pH 5.5, 50°C | Aspergillus niger | |
2.5 | - |
ADP | pH 5.5, 50°C | Aspergillus niger | |
3.8 | - |
1-naphthyl phosphate | pH 5.5, 50°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
x * 108000, SDS-PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Aspergillus niger | - |
? + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
isolated from soil samples collected in Northeast Parana State in Brazil | - |
Aspergillus niger FS3 | - |
isolated from soil samples collected in Northeast Parana State in Brazil | - |
Purification (Comment) | Organism |
---|---|
native enzyme 10.1fold by cationic exchange chromatography, anionic exchange chromatography, and chromatofocusing | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth on citric pulp fermentation | Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
86.1 | - |
purified native enzyme, pH 5.5, 50°C, substrate phytate | Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, purified native enzyme, after 30 days, 88.9% activity are remaining, after 60 days the remaining activity is 84.25% of initial activity, and after 180 days 57.5% | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Aspergillus niger | 1-naphthol + phosphate | - |
? | |
1-naphthyl phosphate + H2O | - |
Aspergillus niger FS3 | 1-naphthol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Aspergillus niger FS3 | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Aspergillus niger | AMP + phosphate | - |
? | |
ADP + H2O | - |
Aspergillus niger FS3 | AMP + phosphate | - |
? | |
ATP + H2O | - |
Aspergillus niger | ADP + phosphate | - |
? | |
ATP + H2O | - |
Aspergillus niger FS3 | ADP + phosphate | - |
? | |
additional information | substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview | Aspergillus niger | ? | - |
? | |
additional information | substrate specificity with substrates Na-phytate, i.e. myo-inositol-1,2,3,4,5,6-hexakisphosphate, naphtyl-1 phosphate, 4-nitrophenylphosphate, ATP, and ADP, overview | Aspergillus niger FS3 | ? | - |
? | |
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 108000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
acid phytase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, residual activity of 86.0%, 68.4%, and 48.2% after 10, 20 or 40 min, respectively | Aspergillus niger |
70 | - |
purified enzyme, 40 min | Aspergillus niger |
80 | - |
purified enzyme, 30 min, 30.5% activity remaining | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus niger |