Any feedback?
Literature summary for 3.1.3.8 extracted from
- Purification and properties of a phytate-degrading enzyme from Pantoea agglomerans (2004), Protein J., 23, 567-576.
General Stability
| General Stability | Organism |
|---|---|
| 5-10% loss of activity after lyophilization | Pantoea agglomerans |
| at protein concentrations below 0.05 mg/ml a rapid loss of stability occurs | Pantoea agglomerans |
| no loss of activity after one freeze-thaw cycle | Pantoea agglomerans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pantoea agglomerans |  |
| Fe3+ | - |
Pantoea agglomerans | |
| myo-inositol hexakisphosphate | inhibition at substrate concentrations above 2.5 mM | Pantoea agglomerans | |
| Zn2+ | - |
Pantoea agglomerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
D-glucose 1-phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
| 0.34 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
| 12.4 | - |
p-nitrophenyl phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pantoea agglomerans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Pantoea agglomerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 1-phosphate + H2O | Pantoea agglomerans | strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate | D-glucose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea agglomerans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pantoea agglomerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23 | - |
- |
Pantoea agglomerans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme retains about 95% of initial activity after 3 months | Pantoea agglomerans |
| 4°C, protein concentration 0.1 mg/ml or greater, half-life is approximately 6 months | Pantoea agglomerans |
| room temperature, protein concentration 0.1 mg/ml or greater, half-life is 10 days | Pantoea agglomerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 1-phosphate + H2O | - |
Pantoea agglomerans | D-glucose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate | Pantoea agglomerans | D-glucose + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Pantoea agglomerans | ? + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Pantoea agglomerans | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Pantoea agglomerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pantoea agglomerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
half-life at room temperature is 10 days | Pantoea agglomerans |
| 55 | - |
90 min, no loss of activity at temperatures below | Pantoea agglomerans |
| 60 | - |
90 min, 13% loss of activity | Pantoea agglomerans |
| 65 | - |
10 min, 29% loss of activity | Pantoea agglomerans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.2 | - |
p-nitrophenyl phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
| 21 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
| 101 | - |
D-glucose 1-phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Pantoea agglomerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | inactive below pH 3.0 or above pH 7.0 | Pantoea agglomerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
4°C, 24 h, 85% loss of activity | Pantoea agglomerans |
| 2.5 | 7.5 | 4°C, stable | Pantoea agglomerans |
| 4.5 | - |
4°C, 10 days, more than 95% of initial activity | Pantoea agglomerans |
| 9 | - |
4°C, 24 h, 70% loss of activity | Pantoea agglomerans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
