Literature summary for 3.1.3.8 extracted from
Lei, X.G.; Kim, T.
Expression of microbial phytases in yeast systems and characterization of the recombinant enzymes (2005), Methods Biotechnol., 17, 209-224.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Pichia pastoris (constitutive or inducible) and Saccharomyces cerevisiae (inducible) |
Aspergillus niger |
Organism
Organism |
UniProt |
Comment |
Textmining |
Aspergillus niger |
P34752 |
3-phytase A precursor |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
recombinant enzyme expressed in yeast is highly glycosylated |
Aspergillus niger |
Purification (Commentary)
Purification (Comment) |
Organism |
partial |
Aspergillus niger |
Synonyms
Synonyms |
Comment |
Organism |
3-phytase A |
- |
Aspergillus niger |
PhyA phytase |
- |
Aspergillus niger |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
55 |
- |
30 min, stable, enzyme expressed in Saccharomyces cerevisiae or Pichia pastoris |
Aspergillus niger |
65 |
- |
30 min, enzyme expressed in Saccharomyces cerevisiae loses about 30% of initial activity, enzyme expressed in Pichia pastoris loses about 45% of initial activity |
Aspergillus niger |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5 |
- |
enzyme expressed in Saccharomyces cerevisiae |
Aspergillus niger |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
3.5 |
6 |
pH 3.5: about 40% of maximal activity, pH 6.0: about 80% of maximal activity, pH 6.5: about 30% of maximal activity. enzyme expressed in Saccharomyces cerevisiae |
Aspergillus niger |