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Literature summary for 3.1.3.8 extracted from
- Isolation and characterization of intracellular phytase from Macrophormina physeolina (1988), Zentralbl. Mikrobiol., 143, 397-403.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dinitrophenol | - |
Macrophomina phaseolina |  |
| arsenate | - |
Macrophomina phaseolina | |
| arsenite | - |
Macrophomina phaseolina | |
| azide | - |
Macrophomina phaseolina | |
| EDTA | - |
Macrophomina phaseolina | |
| F- | - |
Macrophomina phaseolina | |
| Hg2+ | - |
Macrophomina phaseolina | |
| iodoacetic acid | - |
Macrophomina phaseolina | |
| phloridzin | - |
Macrophomina phaseolina | |
| Tartrate | - |
Macrophomina phaseolina | |
| Zn2+ | - |
Macrophomina phaseolina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
myo-inositol hexakisphosphate | - |
Macrophomina phaseolina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Macrophomina phaseolina | |
| K+ | activates | Macrophomina phaseolina | |
| Zn2+ | activates | Macrophomina phaseolina | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | high resistance to the denaturing effect of organic solvents | Macrophomina phaseolina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macrophomina phaseolina | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Macrophomina phaseolina | - |
tassi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Macrophomina phaseolina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.05 M Tris-HCL buffer, pH 8.2,36% loss of activity after 2 weeks | Macrophomina phaseolina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Macrophomina phaseolina | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Macrophomina phaseolina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
60 min, about 95% loss of activity | Macrophomina phaseolina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Macrophomina phaseolina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | 10 min, stable | Macrophomina phaseolina |
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