Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.8 extracted from

  • Pires, A.S.; Cabral, M.G.; Fevereiro, P.; Stoger, E.; Abranches, R.
    High levels of stable phytase accumulate in the culture medium of transgenic Medicago truncatula cell suspension cultures (2008), Biotechnol. J., 3, 916-923.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of phytase in a Medicago truncatula cell suspension line. Recombinant phytase accumulates to at least 25 mg/l and remaines stable along the growth curve, and an enriched fraction with high enzymatic activity is easily obtained Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expression in Medicago truncatula Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
870
-
pH 6.5, 37°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Aspergillus niger