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Literature summary for 3.1.3.8 extracted from
- Production, characteristics and applications of the cell-bound phytase of Pichia anomala (2011), Antonie van Leeuwenhoek, 99, 51-55.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PPHY, DNA sequence, promoter and terminator region, and amino acid sequence determination and analysis | Wickerhamomyces anomalus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Butanedione | - |
Wickerhamomyces anomalus |  |
| guanidine hydrochloride | - |
Wickerhamomyces anomalus | |
| additional information | no inhibition by sodium azide, DTT, 2-mercaptoethanol, EDTA, toluene, glycerol, and PMSF | Wickerhamomyces anomalus | |
| phosphate | - |
Wickerhamomyces anomalus | |
| Triton X-100 | - |
Wickerhamomyces anomalus | |
| Tween 20 | - |
Wickerhamomyces anomalus | |
| Tween 80 | - |
Wickerhamomyces anomalus | |
| Urea | - |
Wickerhamomyces anomalus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | pH 4.0, 60°C | Wickerhamomyces anomalus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell bound | - |
Wickerhamomyces anomalus | - |
- |
| cell wall | about 78% of the enzyme is cell wall bound | Wickerhamomyces anomalus | 5618 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement of metal ions for the activity | Wickerhamomyces anomalus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
6 * 64000, SDS-PAGE | Wickerhamomyces anomalus |
| 384000 | - |
about, native PAGE | Wickerhamomyces anomalus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Wickerhamomyces anomalus | the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents | ? | - |
? | |
| myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Wickerhamomyces anomalus | - |
? + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wickerhamomyces anomalus | D3HIF3 | single copy gene PPHY | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the native enzyme is purified to near homogeneity by acetone precipitation and anion exchange chromatography | Wickerhamomyces anomalus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimization of fermentation for production of cell-bound phytase, overview | Wickerhamomyces anomalus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme, shelf life is 6 months at 4°C, no loss in the activity on lyophilization | Wickerhamomyces anomalus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the cell-bound phytase is able to effectively dephytinize wheat flour, wheat bran, rice flour, and soybean flour, or soymilk, to varied extents | Wickerhamomyces anomalus | ? | - |
? | |
| additional information | the phytase exhibits broad substrate specificity since it hydrolyses 4-nitrophenyl phosphate, ATP, ADP, and glucose-6-phosphate besides phytic acid. The enzyme hydrolyzes insoluble calcium and magnesium phytates efficiently, but not iron phytate | Wickerhamomyces anomalus | ? | - |
? | |
| myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Wickerhamomyces anomalus | ? + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 64000, SDS-PAGE | Wickerhamomyces anomalus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cell-bound phytase | - |
Wickerhamomyces anomalus |
| histidine acid phosphatase | - |
Wickerhamomyces anomalus |
| More | PPHY is a phytase belonging to histidine acid phosphatases | Wickerhamomyces anomalus |
| PPHY | - |
Wickerhamomyces anomalus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified phytase | Wickerhamomyces anomalus |
| 75 | - |
cell bound phytase | Wickerhamomyces anomalus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the cell-bound phytase exhibits better thermostability than the pure enzyme | Wickerhamomyces anomalus |
| 60 | - |
purified enzyme, half-life is 7 days | Wickerhamomyces anomalus |
| 70 | - |
purified enzyme, half-life is 48 h | Wickerhamomyces anomalus |
| 80 | - |
purified enzyme, half-life is 5 min | Wickerhamomyces anomalus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Wickerhamomyces anomalus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Wickerhamomyces anomalus | isoelectric focusing | - |
4.5 |
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