Cloned (Comment) | Organism |
---|---|
cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis | Bos taurus |
genes phyG1 and phyG51, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis | Capra hircus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted phytases PhyG1 and PhyG51 | Capra hircus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Capra hircus | - |
genes phyG1 and phyG51 | - |
Source Tissue | Comment | Organism | Textmining |
---|
Synonyms | Comment | Organism |
---|---|---|
cysteine phytase | - |
Bos taurus |
cysteine phytase | - |
Capra hircus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bos taurus |
50 | - |
- |
Capra hircus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | 80% of maximal activity at 39°C, maximal activity at 50°C | Bos taurus |
37 | 50 | 80% of maximal activity at 39°C, maximal activity at 50°C | Capra hircus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
isozyme r-PhyG1 loses over 80% of its activity after incubation at 50°C for 15 min, whereas isozyme r-phyG51 is not affected by these conditions | Capra hircus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
secreted isozyme PhyG1 | Capra hircus |
6.5 | - |
secreted isozyme PhyG51 | Capra hircus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 | Bos taurus |
5 | 7 | the enzyme retains nearly 100% of their activities after 1 h incubation at 37°C and pH 5.0-7.0 | Capra hircus |
General Information | Comment | Organism |
---|---|---|
physiological function | cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment | Bos taurus |
physiological function | cysteine phytase is the major phytate-degrading enzyme in the anaerobic ruminal environment | Capra hircus |