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Literature summary for 3.1.3.8 extracted from
- Characterization of phytase produced by Aspergillus niger (1997), Folia Microbiol. (Praha), 42, 349-352.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Aspergillus niger |  |
| Cu2+ | - |
Aspergillus niger | |
| F- | - |
Aspergillus niger | |
| Hg2+ | - |
Aspergillus niger | |
| phosphate | - |
Aspergillus niger | |
| Sn2+ | - |
Aspergillus niger | |
| Zn2+ | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger | |
| 0.45 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.2 mM, 12% increase of activity | Aspergillus niger | |
| Mg2+ | 0.2 mM, 6% increase of activity | Aspergillus niger | |
| Mn2+ | 0.2 mM, 59% increase of activity | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45.78 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phosphoglycerate + H2O | - |
Aspergillus niger | glyceric acid + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
| 6-phosphogluconate + H2O | - |
Aspergillus niger | gluconate + phosphate | - |
? | |
| ADP + H2O | - |
Aspergillus niger | ? | - |
? | |
| AMP + H2O | no activity | Aspergillus niger | adenosine + phosphate | - |
? | |
| ATP + H2O | - |
Aspergillus niger | ? | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Aspergillus niger | D-fructose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | - |
Aspergillus niger | D-ribose + phosphate | - |
? | |
| fructose 1,6-diphosphate + H2O | - |
Aspergillus niger | ? | - |
? | |
| glucose 1-phosphate + H2O | - |
Aspergillus niger | glucose + phosphate | - |
? | |
| Glucose 6-phosphate + H2O | - |
Aspergillus niger | Glucose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Aspergillus niger | glycerol + phosphate | - |
? | |
| glycerol 3-phosphate + H2O | - |
Aspergillus niger | glycerol + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | calcium myo-inositol hexakisphosphate | Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | dodecasodium myo-inositol hexakisphosphate | Aspergillus niger | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| phenyl phosphate + H2O | - |
Aspergillus niger | phenol + phosphate | - |
? | |
| riboflavin 5'-phosphate + H2O | - |
Aspergillus niger | riboflavin + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000-100000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 2-7, 2 h, stable | Aspergillus niger |
| 60 | - |
10 min, 5% loss of activity | Aspergillus niger |
| 70 | - |
10 min, 10% loss of activity | Aspergillus niger |
| 80 | - |
10 min, 25% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | 40°C, 2 h, stable | Aspergillus niger |
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