Literature summary for 3.1.3.8 extracted from

Wyss, M.; Pasamontes, L.; Friedlein, A.; Remy, R.; Tessier, M.; Kronenberger, A.; Middendorf, A.; Lehmann, M.; Schoebelen, L.; R�thlisberger, U.; Kusznir, E.; Wahl, G.; Muller, F.; Lahm, H.W.; Vogel, K.; van Loon, A.P.G.M.
Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolase): molecular size, glycosylation pattern, and engineering of proteolytic resistance (1999), Appl. Environ. Microbiol., 65, 359-366.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Aspergillus niger, Saccharomyces cerevisiae or Hansenula polymorpha	Aspergillus fumigatus

Protein Variants

Protein Variants	Comment	Organism
K186G/R187Q	mutant protein with reduced susceptibility to proteolysis	Aspergillus nidulans
R151L	mutation has no effect on specific activity	Aspergillus fumigatus
R151L/S152N	mutant protein with reduced susceptibility to proteolysis	Aspergillus fumigatus
S152N	mutant protein with reduced susceptibility to proteolysis	Aspergillus fumigatus
S152N	mutation has no effect on specific activity	Aspergillus fumigatus

General Stability

General Stability	Organism
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Aspergillus terreus
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Aspergillus fumigatus
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Aspergillus niger
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Thermothelomyces heterothallicus
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Thermomyces dupontii
when expressed in Aspergillus niger, several fungal phytases are susceptible to limited proteolysis by proteases present in culture supernatant	Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39190	-	gel filtration	Escherichia coli
45846	-	1 * 45846, calculation from nucleotide sequence	Escherichia coli
47060	-	analytical ultracentrifugation	Escherichia coli
47270	-	1 * 47270, SDS-PAGE	Escherichia coli
48159	-	1 * 48159, Aspergillus terreus 9A1, calculation from nucleotide sequence	Aspergillus terreus
48276	-	1 * 48276, calculation from nucleotide sequence	Aspergillus fumigatus
48423	-	1 * 48423, Aspergillus niger Naturphos, calculation from nucleotide sequence	Aspergillus niger
49775	-	1 * 49775, calculation from nucleotide sequence	Thermomyces dupontii
50524	-	1 * 50524, calculation from nucleotide sequence	Thermothelomyces heterothallicus
60550	-	1 * 60550, Aspergillus terreus 9A1, SDS-PAGE	Aspergillus terreus
60740	-	analytical ultracentrifugation	Aspergillus nidulans
60770	-	1 * 60770, batch 2, SDS-PAGE	Aspergillus fumigatus
62890	-	1 * 62890, SDS-PAGE	Thermothelomyces heterothallicus
64890	-	Aspergillus niger Natuphos, analytical ultracentrifugation	Aspergillus niger
66150	-	analytical ultracentrifugation	Thermothelomyces heterothallicus
66360	-	1 * 66360, Aspergillus niger Naturphos, SDS-PAGE	Aspergillus niger
66430	-	1 * 66430, SDS-PAGE	Aspergillus nidulans
70380	-	Aspergillus terreus 9A1, analytical ultracentrifugation	Aspergillus terreus
70740	-	analytical ultracentrifugation	Aspergillus fumigatus
71020	-	Aspergillus niger CB, analytical ultracentrifugation	Aspergillus niger
72360	-	1 * 72360, batch 1, SDS-PAGE	Aspergillus fumigatus
73800	-	gel filtration	Thermothelomyces heterothallicus
74530	-	1 * 74530, Aspergillus niger CB, SDS-PAGE	Aspergillus niger
77850	-	batch 1, gel filtration	Aspergillus nidulans
78030	-	Aspergillus terreus CBS, analytical ultracentrifugation	Aspergillus terreus
79900	-	Aspergillus terreus 9A1, gel filtration	Aspergillus terreus
82110	-	1 * 82110, Aspergillus terreus CBS, SDS-PAGE	Aspergillus terreus
82360	-	Aspergillus niger Natuphos, SDS-PAGE	Aspergillus niger
86940	-	batch 2, gel filtration	Aspergillus nidulans
90500	-	gel filtration	Aspergillus fumigatus
103000	-	Aspergillus niger CB, gel filtration	Aspergillus niger
115100	-	Aspergillus terreus CBS, gel filtration	Aspergillus terreus
128400	-	1 * 128400, SDS-PAGE	Thermomyces dupontii
136100	-	analytical ultracentrifugation	Thermomyces dupontii

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	-	-
Aspergillus nidulans	O00093	-	-
Aspergillus niger	P34752	-	-
Aspergillus niger CB	P34752	-	-
Aspergillus niger Naturphos	P34752	-	-
Aspergillus terreus	-	-	-
Aspergillus terreus 9A1	-	-	-
Aspergillus terreus CBS	-	-	-
Escherichia coli	-	The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.	-
Thermomyces dupontii	O00096	-	-
Thermothelomyces heterothallicus	O00107	The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no modification	no glycosylation	Escherichia coli
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Aspergillus terreus
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Aspergillus fumigatus
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Aspergillus niger
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Thermothelomyces heterothallicus
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Thermomyces dupontii
side-chain modification	extent of glycosylation is moderate when the fungal phytases are expressed in filamentous fungi, they are excessive when the phytases are expressed in yeasts	Aspergillus nidulans
side-chain modification	glycosylation sites: Asn residues 27, 105, 207, 230, 339 and 376 are glycosylated in all of the peptides analyzed. Asn59 and Asn120 are both glycosylated in only one of the peptides examined. No glycosylation at Asn352 and Asn338. The incomplete glycosylation of Asn residues 59 and 120 may be one of the reasons for the MW heterogeneity of Aspergillus niger phytase	Aspergillus niger
side-chain modification	glycosylation is moderate in Aspergillus niger, it is excessive and highly variable in Hansenula polymorpha and Saccharomyces cerevisiae	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
myo-inositol hexakisphosphate + H2O	-	Escherichia coli	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus terreus	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus fumigatus	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus niger	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Thermothelomyces heterothallicus	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Thermomyces dupontii	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus nidulans	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus niger Naturphos	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus terreus 9A1	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus niger CB	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Aspergillus terreus CBS	1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 49034-49360, calculation from nucleotide sequence	Aspergillus nidulans
monomer	1 * 49775, calculation from nucleotide sequence	Thermomyces dupontii
monomer	1 * 60550, Aspergillus terreus 9A1, SDS-PAGE	Aspergillus terreus
monomer	1 * 50524, calculation from nucleotide sequence	Thermothelomyces heterothallicus
monomer	1 * 74530, Aspergillus niger CB, SDS-PAGE	Aspergillus niger
monomer	1 * 62890, SDS-PAGE	Thermothelomyces heterothallicus
monomer	1 * 66360, Aspergillus niger Naturphos, SDS-PAGE	Aspergillus niger
monomer	1 * 72360, batch 1, SDS-PAGE	Aspergillus fumigatus
monomer	1 * 66430, SDS-PAGE	Aspergillus nidulans
monomer	1 * 60770, batch 2, SDS-PAGE	Aspergillus fumigatus
monomer	1 * 128400, SDS-PAGE	Thermomyces dupontii
monomer	1 * 82110, Aspergillus terreus CBS, SDS-PAGE	Aspergillus terreus
monomer	1 * 45846, calculation from nucleotide sequence	Escherichia coli
monomer	1 * 47270, SDS-PAGE	Escherichia coli
monomer	1 * 48658-48973, Aspergillus niger CB, calculation from nucleotide sequence	Aspergillus niger
monomer	1 * 48423, Aspergillus niger Naturphos, calculation from nucleotide sequence	Aspergillus niger
monomer	1 * 48570-49166, Aspergillus terreus CBS, calculation from nucleotide sequence	Aspergillus terreus
monomer	1 * 48276, calculation from nucleotide sequence	Aspergillus fumigatus
monomer	1 * 48159, Aspergillus terreus 9A1, calculation from nucleotide sequence	Aspergillus terreus