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Literature summary for 3.1.3.8 extracted from

  • Oh, B.C.; Kim, M.H.; Yun, B.S.; Choi, W.C.; Park, S.C.; Bae, S.C.; Oh, T.K.
    Ca(2+)-inositol phosphate chelation mediates the substrate specificity of beta-propeller phytase (2006), Biochemistry, 45, 9531-9539.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ decreases activity below 0.1 mM or above 2 mM Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.0 mM, required for activity Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-
Bacillus amyloliquefaciens DS11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA superflow resin column chromatography and HiLoad 16/10 phenyl Sepharose column chromatography Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
 Bacillus amyloliquefaciens myo-inositol-2,4,5,6 tetrakisphosphate + phosphate
-
 ?
myo-inositol 1,2,4,5,6-pentakisphosphate + H2O
-
 Bacillus amyloliquefaciens DS11 myo-inositol-2,4,5,6 tetrakisphosphate + phosphate
-
 ?
myo-inositol 2,4,5,6-tetrakisphosphate + H2O
-
 Bacillus amyloliquefaciens myo-inositol 2,4,6-trisphosphate + phosphate
-
 ?
myo-inositol 2,4,5,6-tetrakisphosphate + H2O
-
 Bacillus amyloliquefaciens DS11 myo-inositol 2,4,6-trisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Bacillus amyloliquefaciens myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Bacillus amyloliquefaciens DS11 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
beta-propeller phytase beta-propeller phytase has substrate specificity for myo-inositol hexakisphosphate and yields a myo-inositol trisphosphate and three phosphate groups as the final products Bacillus amyloliquefaciens