Literature summary for 3.1.3.8 extracted from

Fugthong, A.; Boonyapakron, K.; Sornlek, W.; Tanapongpipat, S.; Eurwilaichitr, L.; Pootanakit, K.
Biochemical characterization and in vitro digestibility assay of Eupenicillium parvum (BCC17694) phytase expressed in Pichia pastoris (2010), Protein Expr. Purif., 70, 60-67.

Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Penicillium parvum

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant phytase contains the active site RHGXRXP and HD sequence motifs, a large alpha/beta-domain and a small alpha-domain	Penicillium parvum

General Stability

General Stability	Organism
enzyme is resistant to pepsin	Penicillium parvum

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	5 mM, 50% inhibition	Penicillium parvum
Phenylmethylsulfonylfluoride	1 mM, 17.7% of initial activity	Penicillium parvum
sodium dodecylsulfate	0.5 mM, 0.35% of initial activity	Penicillium parvum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.58	-	myo-inositol hexakisphosphate	sodium salt, pH 5.5, 50°C	Penicillium parvum
0.59	-	myo-inositol hexakisphosphate	calcium salt, pH 5.5, 50°C	Penicillium parvum
10.1	-	4-nitrophenyl phosphate	pH 5.5, 50°C	Penicillium parvum
114.8	-	myo-inositol hexakisphosphate	potassium salt, pH 5.5, 50°C	Penicillium parvum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Penicillium parvum	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	5 mM, 112% of initial activity	Penicillium parvum

Organism

Organism	UniProt	Comment	Textmining
Penicillium parvum	-	-	-
Penicillium parvum BCC1769	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Penicillium parvum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Penicillium parvum	4-nitrophenol + phosphate	-	?
4-nitrophenyl phosphate + H2O	-	Penicillium parvum BCC1769	4-nitrophenol + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Penicillium parvum	1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Penicillium parvum BCC1769	1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Penicillium parvum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	5 min, complete loss of activity	Penicillium parvum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11.8	-	4-nitrophenyl phosphate	pH 5.5, 50°C	Penicillium parvum
103	-	myo-inositol hexakisphosphate	sodium salt, pH 5.5, 50°C	Penicillium parvum
117	-	myo-inositol hexakisphosphate	calcium salt, pH 5.5, 50°C	Penicillium parvum
134	-	myo-inositol hexakisphosphate	potassium salt, pH 5.5, 50°C	Penicillium parvum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Penicillium parvum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.5	8	-	Penicillium parvum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
88.8	-	4-nitrophenyl phosphate	pH 5.5, 50°C	Penicillium parvum
173.7	-	myo-inositol hexakisphosphate	potassium salt, pH 5.5, 50°C	Penicillium parvum
177.9	-	myo-inositol hexakisphosphate	sodium salt, pH 5.5, 50°C	Penicillium parvum
198.9	-	myo-inositol hexakisphosphate	calcium salt, pH 5.5, 50°C	Penicillium parvum

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Release 2023.1 (January 2023)