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Literature summary for 3.1.3.8 extracted from

  • Kim, O.H.; Kim, Y.O.; Shim, J.H.; Jung, Y.S.; Jung, W.J.; Choi, W.C.; Lee, H.; Lee, S.J.; Kim, K.K.; Auh, J.H.; Kim, H.; Kim, J.W.; Oh, T.K.; Oh, B.C.
    beta-Propeller phytase hydrolyzes insoluble Ca2+-phytate salts and completely abrogates the ability of phytate to chelate metal ions (2010), Biochemistry, 49, 10216-10227.
    View publication on PubMed
Search for more literature for 3.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged phytase in Escherichia coli strain BL21(DE3) Hahella chejuensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, best at 3 mM Hahella chejuensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Hahella chejuensis HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ ?
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Hahella chejuensis
-
 ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Hahella chejuensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged phytase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Hahella chejuensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HcBPP preferentially recognizes its substrate and selectively hydrolyzes insoluble Ca2+-phytate salts at three phosphate group sites, yielding the final product, myo-inositol 2,4,6-trisphosphate, exhibiting also the 1- and 5-phytase activities. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ Hahella chejuensis ?
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Hahella chejuensis ? + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
beta-propeller phytase
-
 Hahella chejuensis
BPP
-
 Hahella chejuensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 hydrolysis of Ca2+-phytate Hahella chejuensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 the enzyme is stable at temperatures up to 55°C in the presence of 5 mM Ca2+, but the stability decreases dramatically above 65°C Hahella chejuensis
70
-
 the enzyme retains more than 30% of its activity following incubation at 70°C for 30 min Hahella chejuensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Hahella chejuensis

pH Range

pH Minimum pH Maximum Comment Organism
2 9 the pH profile of the enzyme contains peaks at pH 3.0 and pH 7.0, with the activity at pH 3.0 being approximately 70% of the peak activity at pH 7.0 Hahella chejuensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 the enzyme retains more than 65% of the maximal activity in the pH range of pH 3.0-9.0. In addition, it is stable over the broad pH range of pH 3.0-10.0 Hahella chejuensis

General Information

General Information Comment Organism
physiological function phytate is an antinutritional factor that influences the bioavailability of essential minerals by forming complexes with them and converting them into insoluble salts. beta-Propeller phytase hydrolyzes insoluble Ca2+-phytate salts and completely abrogates the ability of phytate to chelate metal ions, stoichiometry, affinity, and thermodynamics of these interactions by isothermal titration calorimetry, overview. Ins(2,4,6)P3 is unable to bind Ca2+ or any other cation tested, including Co2+, Cu2+, Fe3+, Mg2+, Mn2+, Sr2+, and Zn2+ Hahella chejuensis