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Literature summary for 3.1.3.8 extracted from

  • Azeke, M.; Greiner, R.; Jany, K.
    Purification and characterization of two intracellular phytases from the tempeh fungus Rhizopus oligosporus (2011), J. Food Biochem., 35, 213-227.
    View publication on PubMed
Search for more literature for 3.1.3.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Rhizopus microsporus var. oligosporus
F-
-
 Rhizopus microsporus var. oligosporus
Fe2+ stronger inhibition at 10 mM of isozyme RO2 compared to isozyme RO1 Rhizopus microsporus var. oligosporus
Fe3+ stronger inhibition at 10 mM of isozyme RO2 compared to isozyme RO1 Rhizopus microsporus var. oligosporus
Mg2+
-
 Rhizopus microsporus var. oligosporus
molybdate almost complete inhibition of isozymes RO1 and RO2 at 1-10 mM Rhizopus microsporus var. oligosporus
additional information no or poor inhibition of isozymes RO1 and RO2 by EDTA Rhizopus microsporus var. oligosporus
Zn2+
-
 Rhizopus microsporus var. oligosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate isozyme RO2, pH 4.5, 40°C Rhizopus microsporus var. oligosporus
1.6
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate isozyme RO1, pH 4.5, 40°C Rhizopus microsporus var. oligosporus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Rhizopus microsporus var. oligosporus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, isozymes RO1 and RO2, SDS-PAGE Rhizopus microsporus var. oligosporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Rhizopus microsporus var. oligosporus
-
 ? + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus var. oligosporus
-
 i.e. Rhizopus microsporus var. oligosporus, isozymes RO1 and RO2
-

Purification (Commentary)

Purification (Comment) Organism
two native isozymes RO1 and RO2, 75fold and 46fold, respectively, by acetone fractionation, cation exchange chromatography, gel filtration, and again cation exchange chromatography followed by anion exchange chromatography to homogeneity Rhizopus microsporus var. oligosporus

Source Tissue

Source Tissue Comment Organism Textmining
additional information growth on rice flour suspension or on potato dextrose agar Rhizopus microsporus var. oligosporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
 purified isozyme RO2, pH 4.5, 40°C Rhizopus microsporus var. oligosporus
54
-
 purified isozyme RO1, pH 4.5, 40°C Rhizopus microsporus var. oligosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the phytase isozymes exhibit a broad affinity for various phosphorylated compounds, i.e. 4-nitrophenyl phosphate 1-naphthyl phosphate, 2-naphthyl phosphate, AMP, ATP, GTP, fructose-1, 6-bisphosphate, fructose-6-phosphate, glucose-6-phosphate, and pyridoxal phosphate, substrate specificities, overview Rhizopus microsporus var. oligosporus ?
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Rhizopus microsporus var. oligosporus ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, isozymes RO1 and RO2, SDS-PAGE Rhizopus microsporus var. oligosporus

Synonyms

Synonyms Comment Organism
acid phytase
-
 Rhizopus microsporus var. oligosporus
RO1
-
 Rhizopus microsporus var. oligosporus
RO2
-
 Rhizopus microsporus var. oligosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 isozyme ROII Rhizopus microsporus var. oligosporus
60
-
 isozyme ROI Rhizopus microsporus var. oligosporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 activity profiles of isozymes RO1 and RO2, almost no activity at 75°C for both RO1 and RO2 Rhizopus microsporus var. oligosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 100 thermal inactivation profile of the purified enzyme, 20-25% of maximal activity at 75°C, below 20% of maximal activity remaining at 80°C, inactivation at 100°C, overview Rhizopus microsporus var. oligosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 optimum I for both isozymes RO1 and RO2 Rhizopus microsporus var. oligosporus
4.5
-
 optimum II for isozyme RO1 Rhizopus microsporus var. oligosporus
5
-
 optimum II for isozyme RO2 Rhizopus microsporus var. oligosporus

pH Range

pH Minimum pH Maximum Comment Organism
1 8 pH profile, overview Rhizopus microsporus var. oligosporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 isozyme RO1 is most stable at pH 4.0-4.5 with 102-103% retained activity after 10 days. Above pH 5.0, the stability of RO1 decreases significantly. RO2, on the other hand, is stable over the pH range pH 3.0-5.5 retaining between 72% and 95% activity after 10 days. Less than 20% activity is retained above pH 6.0 Rhizopus microsporus var. oligosporus