Cloned (Comment) | Organism |
---|---|
gene phyA, protease-resistance phytase gene, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Pichia pastoris strain GS115 with secretion to culture supernatant | Penicillium sp. |
Protein Variants | Comment | Organism |
---|---|---|
L151S | the mutation enhances the activity in the range of 37-70°C and pH 2.5-7.0 by facilitating the interaction between the substrate and the catalytic centre | Penicillium sp. |
additional information | replacements of G56E, L65F, Q144H, and L151S improved the thermal stability of the protein by increasing new hydrogen bonds among the adjacent secondary structures | Penicillium sp. |
N354D | the substitution influences the pH profile by weakening the bondage with the side chain of D353, which causes a pKa shift of the catalytic centre | Penicillium sp. |
T11A/G56E/L65F/Q144H/L151S | mutagenesis by Mn2+-dITP random mutation method, the mutant shows improved thermal stability and optimal temperature and pH compared to the wild-type enzyme, the mutant shows high resistance to pepsin | Penicillium sp. |
T11A/H37Y/G56E/L65F/Q144H/L151S/N354D | mutagenesis by Mn2+-dITP random mutation method, the mutant shows improved thermal stability and optimal temperature and pH compared to the wild-type enzyme, the mutant shows high resistance to pepsin | Penicillium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium sp. | A2TEY6 | gene phyA; protease-resistance phytase gene phyA | - |
Purification (Comment) | Organism |
---|---|
secreted recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 culture supernatant | Penicillium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.76 | - |
wild-type enzyme, pH 5.5-6.0, 37°C | Penicillium sp. |
133.3 | - |
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 37°C | Penicillium sp. |
136.6 | - |
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 37°C | Penicillium sp. |
145.9 | - |
mutant T11A/G56E/L65F/Q144H/L151S, pH 5.5-6.0, 50°C | Penicillium sp. |
150.4 | - |
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D, pH 4.8, 50°C | Penicillium sp. |
Synonyms | Comment | Organism |
---|---|---|
protease-resistant phytase | - |
Penicillium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | broad optimum, mutant T11A/G56E/L65F/Q144H/L151S | Penicillium sp. |
37 | 50 | broad optimum, mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D | Penicillium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | temperature profiles of wild-type and mutant enzymes, overview | Penicillium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | temperature profiles of wild-type and mutant enzymes, overview | Penicillium sp. |
100 | - |
5 min, mutant T11A/G56E/L65F/Q144H/L151S retains 72,81% of initial activity, and mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D retains 92.43% of initial activity | Penicillium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
mutant T11A/H37Y/G56E/L65F/Q144H/L151S/N354D | Penicillium sp. |
5.5 | 6 | wild-type enzyme and mutant T11A/G56E/L65F/Q144H/L151S | Penicillium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9 | pH profiles of wild-type and mutant enzymes, no activity of mutant enzymes above pH 7.5, overview | Penicillium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9 | pH profiles of wild-type and mutant enzymes, no activity of mutant enzymes above pH 7.5, overview | Penicillium sp. |
General Information | Comment | Organism |
---|---|---|
additional information | replacements of G56E, L65F, Q144H, and L151S improve the thermal stability of the protein by increasing new hydrogen bonds among the adjacent secondary structures | Penicillium sp. |