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3.2.1.39: glucan endo-1,3-beta-D-glucosidase

This is an abbreviated version!
For detailed information about glucan endo-1,3-beta-D-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.39

Reaction

Laminariheptaose
+ 3 H2O =
laminaritriose
 + 2 laminaribiose

Synonyms

(1-3)-beta-D-glucan endohydrolase, (1-3)-beta-D-glucanase, (1-3)-beta-glucanase GI, (1->3)-beta-glucan endohydrolase, (1->3)-beta-glucan endohydrolase BGN13.1, (1->3)-beta-glucan endohydrolase GI, (1->3)-beta-glucan endohydrolase GII, (1->3)-beta-glucan endohydrolase GIII, (1->3)-beta-glucan endohydrolase GIV, (1->3)-beta-glucan endohydrolase GV, (1->3)-beta-glucan endohydrolase GVI, (1->3)-beta-glucanase, (1->3)-beta-glucanase A1, (1->3)-beta-glucanase BGN13.1, (1->3)-beta-glucanase isoenzyme GI, (1->3)-beta-glucanase isoenzyme GII, (1->3)-beta-glucanase isoenzyme GIII, (1->3)-beta-glucanase isoenzyme GIV, (1->3)-beta-glucanase isoenzyme GV, (1->3)-beta-glucanase isoenzyme GVI, (13)-beta-glucan 3-glucanohydrolase, (13)-beta-glucan endohydrolase, 1,3-beta-D-glucanase, 1,3-beta-glucan 3-glucanohydrolase, 1,3-beta-glucan hydrolase, 1,3-beta-glucanase, 1,3-beta-glucanase I, Acidic beta-1,3-glucanase, acidic endo-beta-1,3-glucanase, AkLam36, Anther-specific protein A6, Ban-Gluc, Basic beta-1,3-endoglucanase BGN13.1, Basic beta-1,3-glucanase, beta-(1-3)-glucanase, beta-1,3 glucanase, beta-1,3-D-glucanase, beta-1,3-endoglucanase, Beta-1,3-endoglucanase GI, Beta-1,3-endoglucanase GII, Beta-1,3-endoglucanase GIII, Beta-1,3-endoglucanase GIV, Beta-1,3-endoglucanase GV, Beta-1,3-endoglucanase GVI, Beta-1,3-endoglucanase, basic, beta-1,3-glucan hydrolase, beta-1,3-glucanase, beta-1,3-glucanase I, beta-1,3-glucanase II, beta-glucanase, Bgl, Bgl2, Bgl64A, BglF, BglII, BglS27, Bglu50A, Bgt17AE158A, CaENG, callase, CC1G_04051, CcGluE, Cel17A, DEHA2G18766g, DEHA2G18766p, EGase1, EGase2, EGase3, endo-(1,3)-beta-D-glucanase, endo-(1-3)-beta-glucanase, endo-(13)-beta-D-glucanase, endo-1,3-beta-D-glucanase, endo-1,3-beta-glucanase, endo-1,3-beta-glucosidase, endo-1,3-glucanase, endo-1,3;4-beta-glucanase, endo-beta-1,3(4)-glucanase, endo-beta-1,3-D-glucanase, endo-beta-1,3-glucanase, endo-beta-1->3-glucanase, endo-type beta-1,3-glucanase, Endo23, Eng, eng1, ENG16A, Eng2, Eng2 protein, EngA, Engl1, exo-beta-1,3-glucanase, Fra e 9.01 allergen, Gbl2a, Gbl2b, Gbl2c, Gbl2d, Gbl2e, Gbl2f, Gbl3, Gfa, GH family 81 beta-1,3-glucanase, GLU, Glu1, GluA, GLUB20-2, glucan endo-1,3-beta-glucosidase, Glucanase GLA, Glucanase GLB, glucanase LIV, glucanase Lo, GLUD2, HdLam33, kitalase, LAM, Lam81A, LamA, laminaranase, laminarinase, Lic16A, lytic beta-(1-3)-glucanase I, lytic beta-(1-3)-glucanase II, Mo enzyme, More, oligo-1,3-glucosidase, OsGlu1, OsGlu2, PCEng2p, PfLamA, PpGns1, PR-2B, PR-35, PR-36, PR-37, Rv0315, SLam, SpsLamIV, TLam, TmLam, VIT_05s0077g01150, VIT_205s0077g01150, VIT_206s0061g00120, VIT_208s0007g06060, YlCrh1Sp, YlCrh2Sp, zerzaust, ZET, ZgLamA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.39 glucan endo-1,3-beta-D-glucosidase

Purification

Purification on EC 3.2.1.39 - glucan endo-1,3-beta-D-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-Sepharose column chromatography
-
ammonium sulfate precipitation and His affinity chromatography using ProBond nickel-chelating resin
-
ammonium sulfate precipitation, ion-exchange and gel filtration
-
both native enzyme and recombinant catalytic domain
-
CM-Sephadex C-50 gel filtration, phenyl-Sepharose CL-4B column chromatography, and Sephadex G-75 gel filtration
-
CM-Sepharose Fast Flow column chromatography
-
DEAE-cellulose column chromatography, Ni-NTA agarose column chromatography, and Sephacryl S-100 gel filtration
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and phenyl-Toyopearl column chromatography
from carbon-starving culture
-
from fruiting body
from larval midgut
in crystalline form
isoform Bgl2 copurifies with acid trehalase
-
native enzyme 113fold from midgut to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration
native enzyme 126fold from digestive fluid by ammonium sulfate fractionation, three steps of cation exchange chromatography, ultrafiltration and gel filtration
-
native enzyme from mantle by ammonium sulfate fractionation, dialysis, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration, and dialysis, to homogeneity
-
native extracellular enzyme from culture filtrate by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
-
native HdLam33 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration
native LamA from culture medium by hydrophobic interaction chromatography and gel filtration
-
Ni-IDA column chromatography and Sephacryl S-100 gel filtration
Ni-IDA column chromatography, and Sephacryl S-100 gel filtration
Ni-NTA column chromatography
Ni-NTA resin column chromatography, and Superdex 200 gel filtration
nickel metal affinity chromatography
overexpressed enzyme, partial
-
partial
precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C
-
Q-Sepharose column chromatography
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme from Escherichia coli strain BL21 cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, nickel affinity chromatography, and dialysis
recombinant enzyme with His6-tag, expression in Escherichia coli
-
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzymes from Escherichia coli strain M15 by nickel affinity chromatography
-
recombinant His-tagged mutant E259A from Escherichia coli strain BL21 to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ncikel affiniyt chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His-tagged wild-type and mutant LamA discoidin domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli by nickel affinity chromatography, ultrafiltration, tag cleavage by Bacillus subtilis proteases, and gel filtration
recombinant His6-tagged BglS27 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged PCEng2 from Saccharomyces cerevisiae by immunoaffinity chromatography and nickel affinity chromatography
Sephacryl S-200 gelfiltration and Phenyl Sepharose CL 4B chromatography
-
to homogeneity
to homogeneity by absortion chromatography for lytic beta-(1-3)-glucanase I, partial for lytic beta-(1-3)-glucanase II
-
to homogeneity, 1step chromatography
-
to homogeneity, 2step chromatography
to homogeneity, 2step chromatography, isoelectric focusing
-
to homogeneity, 3step chromatography
to homogeneity, 3step chromatography for glucanase I, 4step chromatography for glucanase II
-
to homogeneity, 3step chromatography, isoelectric focusing
-
to homogeneity, 4-5step chromatography
-
to homogeneity, 4step chroamtography
-
to homogeneity, 4step chromatography
to homogeneity, 5step chromatography
-
to homogeneity, 5step chromatography, affinity chromatography
-
to homogeneity, 6step chromatography
-
to homogeneity, affinity chromatography
to homogeneity, chromatography steps
-
to homogeneity, chromatography, isoelectric focusing
-
to homogeneity, chromatography, preparative gel electrophoresis
-
to homogeneity, continuous-curtain electrophoresis
-
to homogeneity, crystalline form
Fungi imperfecti
-
to homogeneity, gel filtration, preparative PAGE
-
to homogeneity, repeated gel filtration steps
-
to homogeneity, separation of four different beta-1,3-glucanases
-
to homogeneity, three forms separated, L-1, L-2 and L-4, chromatography steps, affinity chromatography
-
to homogene¡ty, affinity chromatography
-
Toyopearl DEAE-650M column chromatography, Toyopearl Q-600C AR column chromatography, and Superdex 200 gel filtration
-