3.2.1.39: glucan endo-1,3-beta-D-glucosidase This is an abbreviated version! For detailed information about glucan endo-1,3-beta-D-glucosidase, go to the full flat file .
Reaction
Laminariheptaose +
3 H2O
=
laminaritriose +
2 laminaribiose
Synonyms (1-3)-beta-D-glucan endohydrolase, (1-3)-beta-D-glucanase, (1-3)-beta-glucanase GI, (1->3)-beta-glucan endohydrolase, (1->3)-beta-glucan endohydrolase BGN13.1, (1->3)-beta-glucan endohydrolase GI, (1->3)-beta-glucan endohydrolase GII, (1->3)-beta-glucan endohydrolase GIII, (1->3)-beta-glucan endohydrolase GIV, (1->3)-beta-glucan endohydrolase GV, (1->3)-beta-glucan endohydrolase GVI, (1->3)-beta-glucanase, (1->3)-beta-glucanase A1, (1->3)-beta-glucanase BGN13.1, (1->3)-beta-glucanase isoenzyme GI, (1->3)-beta-glucanase isoenzyme GII, (1->3)-beta-glucanase isoenzyme GIII, (1->3)-beta-glucanase isoenzyme GIV, (1->3)-beta-glucanase isoenzyme GV, (1->3)-beta-glucanase isoenzyme GVI, (13)-beta-glucan 3-glucanohydrolase, (13)-beta-glucan endohydrolase, 1,3-beta-D-glucanase, 1,3-beta-glucan 3-glucanohydrolase, 1,3-beta-glucan hydrolase, 1,3-beta-glucanase, 1,3-beta-glucanase I, Acidic beta-1,3-glucanase, acidic endo-beta-1,3-glucanase, AkLam36, Anther-specific protein A6, Ban-Gluc, Basic beta-1,3-endoglucanase BGN13.1, Basic beta-1,3-glucanase, beta-(1-3)-glucanase, beta-1,3 glucanase, beta-1,3-D-glucanase, beta-1,3-endoglucanase, Beta-1,3-endoglucanase GI, Beta-1,3-endoglucanase GII, Beta-1,3-endoglucanase GIII, Beta-1,3-endoglucanase GIV, Beta-1,3-endoglucanase GV, Beta-1,3-endoglucanase GVI, Beta-1,3-endoglucanase, basic, beta-1,3-glucan hydrolase, beta-1,3-glucanase, beta-1,3-glucanase I, beta-1,3-glucanase II, beta-glucanase, Bgl, Bgl2, Bgl64A, BglF, BglII, BglS27, Bglu50A, Bgt17AE158A, CaENG, callase, CC1G_04051, CcGluE, Cel17A, DEHA2G18766g, DEHA2G18766p, EGase1, EGase2, EGase3, endo-(1,3)-beta-D-glucanase, endo-(1-3)-beta-glucanase, endo-(13)-beta-D-glucanase, endo-1,3-beta-D-glucanase, endo-1,3-beta-glucanase, endo-1,3-beta-glucosidase, endo-1,3-glucanase, endo-1,3;4-beta-glucanase, endo-beta-1,3(4)-glucanase, endo-beta-1,3-D-glucanase, endo-beta-1,3-glucanase, endo-beta-1->3-glucanase, endo-type beta-1,3-glucanase, Endo23, Eng, eng1, ENG16A, Eng2, Eng2 protein, EngA, Engl1, exo-beta-1,3-glucanase, Fra e 9.01 allergen, Gbl2a, Gbl2b, Gbl2c, Gbl2d, Gbl2e, Gbl2f, Gbl3, Gfa, GH family 81 beta-1,3-glucanase, GLU, Glu1, GluA, GLUB20-2, glucan endo-1,3-beta-glucosidase, Glucanase GLA, Glucanase GLB, glucanase LIV, glucanase Lo, GLUD2, HdLam33, kitalase, LAM, Lam81A, LamA, laminaranase, laminarinase, Lic16A, lytic beta-(1-3)-glucanase I, lytic beta-(1-3)-glucanase II, Mo enzyme, More, oligo-1,3-glucosidase, OsGlu1, OsGlu2, PCEng2p, PfLamA, PpGns1, PR-2B, PR-35, PR-36, PR-37, Rv0315, SLam, SpsLamIV, TLam, TmLam, VIT_05s0077g01150, VIT_205s0077g01150, VIT_206s0061g00120, VIT_208s0007g06060, YlCrh1Sp, YlCrh2Sp, zerzaust, ZET, ZgLamA
ECTree
Inhibitors
Inhibitors on EC 3.2.1.39 - glucan endo-1,3-beta-D-glucosidase
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(2,3)-epoxypropyl beta-D-laminaribiose
-
number of glucosyl residues in the inhibitor affects aglycon chain length specificity and correct positioning of the substrate at the active site
1-ethyl-3(-dimethylaminopropyl)carbodiimide
-
85% residual activity at 10 mM (pH 5.0)
2-Hydroxy-5-nitrobenzylbromide
3,4-epoxybutyl-beta-D-cellobioside
-
-
Ba2+
-
40-50% inhibition at 2 mM
carbodiimide
-
inhibition at 100 mM
Cr2(SO4)3
-
36% inhibition at 1 mM
Cr3+
loss of 11.6% activity at 5 mM
diethyl dicarbonate
-
complete loss of activity at 36 mM (pH 6.0)
GSSG
-
slight inhibition at 1 mM
hexadecyltrimethylammonium bromide
K3Fe(CN)6
Flavobacterium dormitator
-
slight inhibition at 1 mM
KBr
-
slight inhibition at 1 mM
Lespedeza cuneata cellulase inhibitor
-
inhibition at 0.2 mg/ml
-
monoiodoacetate
34% inhibition at 5 mM
N-Acetylimidazole
-
inhibition at 23 mM
N-ethylmaleimide
-
slight inhibition at 1 mM
Na+
24% inhibition at 0.7 M
Na2WO4
-
36% inhibition at 1 mM
NaN3
Flavobacterium dormitator
-
slight inhibition at 1 mM
p-hydroxymercuribenzoate
-
strong inhibition at 1 mM
Pd2+
23% inhibition at 1 mM
propylene oxide
-
15% residual activity at 6 M (pH 4.0)
Semicarbazide
Flavobacterium dormitator
-
slight inhibition at 1 mM
2-Hydroxy-5-nitrobenzylbromide
47% inhibition at 5 mM
2-Hydroxy-5-nitrobenzylbromide
-
inhibition at 10 mM
2-mercaptoethanol
-
41% residual activity at 10 mM
2-mercaptoethanol
33.7% residual activity at 10 mM
2-mercaptoethanol
-
1 mM concentration, activity loss 49%
4-chloromercuribenzoate
-
strong inhibition at 1 mM
4-chloromercuribenzoate
-
45% inhibition at 1 mM
4-chloromercuribenzoate
Flavobacterium dormitator
-
45% inhibition at 1 mM
4-chloromercuribenzoate
-
slight inhibition on laminarin hydrolysis, strong inhibition on disruption of living cells
AgNO3
-
inhibition at 1 mM
AgNO3
-
complete inhibition at 1 mM
AgNO3
-
inhibition on both laminarin hydrolysis and disruption of living yeast cells
Ca2+
-
slight inhibition at 1 mM
Ca2+
-
45% inhibition at 1 mM
Ca2+
-
84% residual activity at 10 mM
Ca2+
86.7% residual activity at 10 mM
Ca2+
-
slight inhibition at 1 mM
Ca2+
loss of 11.5% activity at 5 mM
Co2+
-
94% residual activity at 10 mM
Co2+
80.8% residual activity at 1 mM
Co2+
-
1 mM, 40-50% inhibition
Co2+
-
slight inhibition at 1 mM
Cu2+
1-5 mM, strong
Cu2+
-
43% inhibition at 1 mM
Cu2+
-
40-50% inhibition at 2 mM
Cu2+
-
strong inhibition at 1 mM
Cu2+
-
86% residual activity at 10 mM
Cu2+
86% inhibition at 1 mM
Cu2+
-
1 mM, 40-50% inhibition
Cu2+
Flavobacterium dormitator
-
strong inhibition at 1 mM
Cu2+
-
inhibition at 20 mM
Cu2+
-
inhibition at 1 mM
Cu2+
-
inhibition at 1 mM
Cu2+
-
55% inhibition at 1 mM
Cu2+
-
reduced activity to 41% and 50% at 1 and 5 mM
Cu2+
loss of 24.4% activity at 5 mM
Cu2+
-
21% inhibition at 2 mM
dithiothreitol
-
35% residual activity at 10 mM
dithiothreitol
29.6% residual activity at 10 mM
EDTA
-
nearly complete inhibition at 1 mM
EDTA
-
40% inhibition at 1 mM
EDTA
-
31% residual activity at 10 mM
EDTA
13.2% residual activity at 10 mM
EDTA
Flavobacterium dormitator
-
nearly complete inhibition at 1 mM
EDTA
-
1 mM concentration, activity loss 28%
Fe2+
1-5 mM, strong
Fe2+
-
strong inhibition at 1 mM
Fe2+
-
8.4% residual activity at 10 mM
Fe2+
5.9% residual activity at 10 mM
Fe2+
20% inhibition at 1 mM
Fe2+
-
1 mM, 40-50% inhibition
Fe2+
loss of 22.6% activity at 5 mM
Fe2+
-
5 mM, 77% residual activtiy
Fe3+
-
6.5% residual activity at 10 mM
Fe3+
10.1% residual activity at 10 mM
Fe3+
72% inhibition at 1 mM
hexadecyltrimethylammonium bromide
-
strong inhibition at 1 mM
hexadecyltrimethylammonium bromide
-
50% inhibition at 1 mM
Hg2+
1-5 mM, strong
Hg2+
-
52% inhibition for laminarin, 62% inhibition for pustulan
Hg2+
-
marked inhibition at 1 mM
Hg2+
-
inhibition at 0.4 mM
Hg2+
-
marked inhibition at 1 mM
Hg2+
Flavobacterium dormitator
-
36% inhibition at 1 mM
Hg2+
39% inhibition at 1 mM
Hg2+
-
inhibition at 0.2 mM
Hg2+
-
inhibition at 1 mM
Hg2+
-
marked inhibition at 1 mM
Hg2+
-
marked inhibition at 1 mM
Hg2+
-
marked inhibition at 1 mM
Hg2+
-
inhibition on both laminarin hydrolysis and disruption of living yeast cells
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
inhibition at 1 mM
Hg2+
-
strong inhibition at 2 mM
Hg2+
-
5 mM, 93% inhibition
Hg2+
-
5 mM, no residual activtiy
Iodine
Flavobacterium dormitator
-
30% inhibition at 1 mM
Iodine
-
inhibition at 2 mM
iodoacetamide
-
slight inhibition
iodoacetamide
-
slight inhibition
KMnO4
-
55% inhibition for laminarin, 85% inhibition for pustulan
KMnO4
Flavobacterium dormitator
-
complete inhibition at 1 mM
KMnO4
-
nearly complete inhibition of laminarin hydrolysis and disruption of living yeast cells
Laminarin
-
Laminarin
-
25% inhibition above 2.5 mg/ml for beta-glucanase IIIB
Laminarin
-
slight inhibition above 1 mg/ml for glucanase I, above 0.5 mg/ml inhibitory for glucanase II
Li+
-
51% residual activity at 10 mM
Li+
51.3% residual activity at 10 mM
Mg2+
-
slight inhibition at 1 mM
Mg2+
-
slight inhibition at 1 mM
Mg2+
-
50% residual activity at 10 mM
Mg2+
61.3% residual activity at 10 mM
Mg2+
-
slight inhibition at 1 mM
Mg2+
-
inhibition on hydrolysis of laminarin, not on disruption of living yeast cells
Mn2+
-
72% inhibition at 1 mM
Mn2+
-
complete inhibition at 1 mM
Mn2+
-
55% residual activity at 10 mM
Mn2+
68.1% residual activity at 10 mM
Mn2+
-
1 mM, 40-50% inhibition
Mn2+
-
slight inhibition in hydrolysis of laminarin, not on disruption of living yeast cells
Mn2+
loss of 24.2% activity at 5 mM
N-bromosuccinimide
-
-
N-bromosuccinimide
-
complete loss of activity at 0.1 mM (pH 4.0)
N-bromosuccinimide
-
inhibition at 5 mM
NaAsO2
Flavobacterium dormitator
-
slight inhibition at 1 mM
NaAsO2
-
39% inhibition at 1 mM
Ni2+
-
1 mM, 85% inhibition
Ni2+
Flavobacterium dormitator
-
slight inhibition at 1 mM
Pb2+
Flavobacterium dormitator
-
59% inhibition at 1 mM
Pb2+
-
inhibition at 1 mM
Pb2+
-
45% inhibition at 1 mM
Pb2+
-
inhibition on hydrolysis of laminarin, only slight inhibition in disruption of living yeast cells
Phenylmercurinitrate
-
-
Phenylmercurinitrate
-
inhibition at 2 mM
SDS
-
52% inhibition at 1 mM
SDS
-
complete inhibition at 1 mM
SDS
94.5% inhibition at 1 mM
SDS
-
43% inhibition at 2 mM
Sn2+
-
25% inhibition at 1 mM
Sn2+
-
5 mM, 86% residual activtiy
Sodium 7-deoxycholate
-
38% inhibition at 1 mM
Sodium 7-deoxycholate
-
55% inhibition at 1 mM
Zn2+
1-5 mM, strong
Zn2+
-
40-50% inhibition at 2 mM
Zn2+
-
complete inhibition at 1 mM
Zn2+
-
35% residual activity at 10 mM
Zn2+
41.7% residual activity at 10 mM
Zn2+
Flavobacterium dormitator
-
25% inhibition at 1 mM
Zn2+
-
59% inhibition at 1 mM
Zn2+
loss of 22.0% activity at 5 mM
additional information
not inhibitory: dithiothreitol, EDTA, ethanol up to 10%, SDS up to 20 mM, NaCl up to 50 mM
-
additional information
-
not inhibitory: dithiothreitol, EDTA, ethanol up to 10%, SDS up to 20 mM, NaCl up to 50 mM
-
additional information
not influenced by K+, Na+, or NH4+
-
additional information
poor or no effects by 1 mM of DTT, urea, EDTA, Triton X-100, and Tween20
-
additional information
-
poor or no effects by 1 mM of DTT, urea, EDTA, Triton X-100, and Tween20
-
additional information
-
not inhibitory: NaCl up to 100 mM, or 1 mM MgCl2, KCl, CaCl2 and ZnSO4
-
additional information
-
not inhibited by EDTA, p-chloromercuribenzoate, iodoacetamide, N-ethylmaleimide, Ellman's reagent, N-acetylimidazole, tetranitromethane, 2,4-pentanedione, and trinitrobenzolsulfonic acid
-
additional information
-
Phytophthora capsici secretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. Inhibition by glucanase inhibitor protein is mainly caused by recognition of an arginine as well as an isoleucine residue during the interaction of the two proteins, molecular docking studies
-
additional information
-
cobalt and ferric ions do not inhibit enzyme activity, ions of magnesium, zinc, copper, calcium, nickel and manganese show a remaining activity between 65 and 90%
-
additional information
-
Ca2+, Cu2+,Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity
-
additional information
-
Ca2+, Cu2+, Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity
-
additional information
the enzyme is not inhibited by high substrate concentrations
-
additional information
-
the enzyme is not inhibited by high substrate concentrations
-
additional information
-
not inhibitory: glucono-delta-lactone
-
additional information
-
not inhibitory: glucono-delta-lactone
-