3.2.1.39: glucan endo-1,3-beta-D-glucosidase This is an abbreviated version! For detailed information about glucan endo-1,3-beta-D-glucosidase, go to the full flat file .
Reaction
Laminariheptaose +
3 H2O
=
laminaritriose +
2 laminaribiose
Synonyms (1-3)-beta-D-glucan endohydrolase, (1-3)-beta-D-glucanase, (1-3)-beta-glucanase GI, (1->3)-beta-glucan endohydrolase, (1->3)-beta-glucan endohydrolase BGN13.1, (1->3)-beta-glucan endohydrolase GI, (1->3)-beta-glucan endohydrolase GII, (1->3)-beta-glucan endohydrolase GIII, (1->3)-beta-glucan endohydrolase GIV, (1->3)-beta-glucan endohydrolase GV, (1->3)-beta-glucan endohydrolase GVI, (1->3)-beta-glucanase, (1->3)-beta-glucanase A1, (1->3)-beta-glucanase BGN13.1, (1->3)-beta-glucanase isoenzyme GI, (1->3)-beta-glucanase isoenzyme GII, (1->3)-beta-glucanase isoenzyme GIII, (1->3)-beta-glucanase isoenzyme GIV, (1->3)-beta-glucanase isoenzyme GV, (1->3)-beta-glucanase isoenzyme GVI, (13)-beta-glucan 3-glucanohydrolase, (13)-beta-glucan endohydrolase, 1,3-beta-D-glucanase, 1,3-beta-glucan 3-glucanohydrolase, 1,3-beta-glucan hydrolase, 1,3-beta-glucanase, 1,3-beta-glucanase I, Acidic beta-1,3-glucanase, acidic endo-beta-1,3-glucanase, AkLam36, Anther-specific protein A6, Ban-Gluc, Basic beta-1,3-endoglucanase BGN13.1, Basic beta-1,3-glucanase, beta-(1-3)-glucanase, beta-1,3 glucanase, beta-1,3-D-glucanase, beta-1,3-endoglucanase, Beta-1,3-endoglucanase GI, Beta-1,3-endoglucanase GII, Beta-1,3-endoglucanase GIII, Beta-1,3-endoglucanase GIV, Beta-1,3-endoglucanase GV, Beta-1,3-endoglucanase GVI, Beta-1,3-endoglucanase, basic, beta-1,3-glucan hydrolase, beta-1,3-glucanase, beta-1,3-glucanase I, beta-1,3-glucanase II, beta-glucanase, Bgl, Bgl2, Bgl64A, BglF, BglII, BglS27, Bglu50A, Bgt17AE158A, CaENG, callase, CC1G_04051, CcGluE, Cel17A, DEHA2G18766g, DEHA2G18766p, EGase1, EGase2, EGase3, endo-(1,3)-beta-D-glucanase, endo-(1-3)-beta-glucanase, endo-(13)-beta-D-glucanase, endo-1,3-beta-D-glucanase, endo-1,3-beta-glucanase, endo-1,3-beta-glucosidase, endo-1,3-glucanase, endo-1,3;4-beta-glucanase, endo-beta-1,3(4)-glucanase, endo-beta-1,3-D-glucanase, endo-beta-1,3-glucanase, endo-beta-1->3-glucanase, endo-type beta-1,3-glucanase, Endo23, Eng, eng1, ENG16A, Eng2, Eng2 protein, EngA, Engl1, exo-beta-1,3-glucanase, Fra e 9.01 allergen, Gbl2a, Gbl2b, Gbl2c, Gbl2d, Gbl2e, Gbl2f, Gbl3, Gfa, GH family 81 beta-1,3-glucanase, GLU, Glu1, GluA, GLUB20-2, glucan endo-1,3-beta-glucosidase, Glucanase GLA, Glucanase GLB, glucanase LIV, glucanase Lo, GLUD2, HdLam33, kitalase, LAM, Lam81A, LamA, laminaranase, laminarinase, Lic16A, lytic beta-(1-3)-glucanase I, lytic beta-(1-3)-glucanase II, Mo enzyme, More, oligo-1,3-glucosidase, OsGlu1, OsGlu2, PCEng2p, PfLamA, PpGns1, PR-2B, PR-35, PR-36, PR-37, Rv0315, SLam, SpsLamIV, TLam, TmLam, VIT_05s0077g01150, VIT_205s0077g01150, VIT_206s0061g00120, VIT_208s0007g06060, YlCrh1Sp, YlCrh2Sp, zerzaust, ZET, ZgLamA
ECTree
Subunits
Subunits on EC 3.2.1.39 - glucan endo-1,3-beta-D-glucosidase
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x * 148000, deduced from gene sequence, x * 125000, SDS-PAGE
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x * 148000, deduced from gene sequence, x * 125000, SDS-PAGE
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x * 71000, SDS-PAGE
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x * 51030, mass spectrometry, x * 40890, sequence calculation
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x * 65993, calculated, x * 150000, SDS-PAGE of recombinant protein, due to hyperglycosylation
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x * 27790, sequence calculation
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x * 24000, SDS-PAGE
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x * 124157, calculated
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x * 43000, recombinant protein with his6-tag, SDS-PAGE
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x * 41000, SDS-PAGE, x * 40798, MALDI-TOF
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x * 44344, sequence calculation, x * 44000, recombinant enzyme, SDS-PAGE
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x * 41000, SDS-PAGE, x * 40798, MALDI-TOF
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x * 44344, sequence calculation, x * 44000, recombinant enzyme, SDS-PAGE
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x * 60000, SDS-PAGE
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x * 45000, SDS-PAGE
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x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE
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x * 31500, SDS-PAGE
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x * 31500, SDS-PAGE
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x * 31500, SDS-PAGE
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x * 31500, SDS-PAGE
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x * 31500, SDS-PAGE
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x * 29000, SDS-PAGE
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x * 60000-90000, SDS-PAGE
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x * 60000-90000, SDS-PAGE
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x * 40000, SDS-PAGE and calculated
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x * 61000, SDS-PAGE
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x * 47760, calculated, x * 45000, SDS-PAGE
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x * 29746, calculated from amino acid sequence
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x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation
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x * 39836, calculated from amino acid sequence
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x * 36000, SDS-PAGE
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x * 39836, calculated from amino acid sequence
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x * 25000, SDS-PAGE, x * 26000, calculated
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x * 37000, recombinant His-tagged enzyme, SDS-PAGE
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x * 75000, recombinant His6-tagged enzyme, SDS-PAGE
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x * 90000, calculated, x * 105000, SDS-PAGE
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x * 90000, calculated, x * 105000, SDS-PAGE
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x * 38000, mature protein, deduced from gene sequence
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x * 30135, mass spectrometry
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x * 31000, SDS-PAGE
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x * 37000-41000, SDS-PAGE
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x * 52780, calculated from amino acid sequence
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x * 49000, deduced from gene sequence
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x * 42700, about, sequence calculation
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x * 36000, SDS-PAGE
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x * 50000, SDS-PAGE, x * 40117, sequence calculation
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x * 24000, SDS-PAGE
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x * 32550, sequence calculation
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x * 37000, SDS-PAGE, x * 37100, calculated
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x * 39000, SDS-PAGE, x * 38500, calculated
dimer
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2 * 91000, SDS-PAGE
dimer
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2 * 30000, non-reducing PAGE
dimer
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1 * 78500, 1 * 82000, glucanase II, SDS-PAGE
monomer
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1 * 32500, SDS-PAGE
monomer
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1 * 74000, SDS-PAGE, gel filtration
monomer
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1 * 36000, SDS-PAGE
monomer
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1 * 32000, isoform GI, SDS-PAGE, gel filtration
monomer
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1 * 35000, isoform GII, SDS-PAGE, gel filtration
monomer
Littorina kurila
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1 * 40000, SDS-PAGE
monomer
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1 * 30000, gel filtration, SDS-PAGE
monomer
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1 * 32000-33000, SDS-PAGE, sedimentation equilibrium
monomer
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1 * 29000, SDS-PAGE, gel filtration
monomer
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1 * 65000-68000, SDS-PAGE, gel filtration
monomer
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1 * 42000-43000, SDS-PAGE, gel filtration
monomer
1 * 79300, calculated from amino acid sequence
monomer
1 * 86900, SDS-PAGE
monomer
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1 * 79300, calculated from amino acid sequence
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monomer
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1 * 86900, SDS-PAGE
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monomer
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1 * 75000, glucanase I, SDS-PAGE, gel filtration
monomer
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1 * 75000-79000, gel filtration, SDS-PAGE
monomer
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1 * 99000, recombinant enzyme, SDS-PAGE
monomer
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1 * 34700, gel filtration
additional information
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secondary structure analysis: alpha-helix comprising 55.6% of the structure, beta-sheet 22.4%, and random coil structures 22.0%. The total content of alpha-helix and beta-sheet accounts for more than 75.0% of the secondary structure, amino acid sequence comparisons
additional information
structure comparisons
additional information
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structure comparisons
additional information
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structure comparisons
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additional information
the enzyme contains a putative signal peptide and a typical motif of glycosyl hydrolase family 16, EIDITE, CaLam homology modelling, overview
additional information
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the enzyme contains a putative signal peptide and a typical motif of glycosyl hydrolase family 16, EIDITE, CaLam homology modelling, overview
additional information
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41000 SDS-PAGE, 71000 gel filtration chromatography, lamarinase is a dimer or contains a laminarin-binding domain, which is in similar size to the catalytic compound
additional information
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41000 SDS-PAGE, 71000 gel filtration chromatography, lamarinase is a dimer or contains a laminarin-binding domain, which is in similar size to the catalytic compound
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additional information
putative functional units of the single protein strain include, from N to C terminus, a leader peptide, three repeats of the S-layer homologous module, a catalytic module of glycoside hydrolase family 16, four repeats of the carbohydrate-binding module of family CBM_4_9, and an analogue of coagulation factor Fa5/8C
additional information
the beta-1,3-glucanase LamA is highly modular, containing a signal sequence, three repeats of the S-layer homologous module, a segment with unknown function, a catalytic module of glycoside hydrolase family 16, GH16, four repeats of CBM4 family, and a F5/8C module representing a discoidin domain, DS domain, from N- to C-terminus. The recombinant isolated purified His-tagged DS domain forms dimers
additional information
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the beta-1,3-glucanase LamA is highly modular, containing a signal sequence, three repeats of the S-layer homologous module, a segment with unknown function, a catalytic module of glycoside hydrolase family 16, GH16, four repeats of CBM4 family, and a F5/8C module representing a discoidin domain, DS domain, from N- to C-terminus. The recombinant isolated purified His-tagged DS domain forms dimers
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additional information
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LamA NMR, differential scanning calorimetry, and mass spectrometry structure determination and analysis, modelling, overview
additional information
the enzyme monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them, three-dimensional structure of enzyme RmLam81A domain A, domain B and domain C, overview
additional information
the enzyme monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them, three-dimensional structure of enzyme RmLam81A domain A, domain B and domain C, overview
additional information
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the enzyme monomer consists of an N-terminal beta-sandwich domain, a C-terminal (alpha/alpha)6 domain and an additional domain between them, three-dimensional structure of enzyme RmLam81A domain A, domain B and domain C, overview
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additional information
enzyme has two functional domain, N-terminal part has enzymic activity, C-terminal part is a putative carbohydrate-binding module
additional information
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enzyme has two functional domain, N-terminal part has enzymic activity, C-terminal part is a putative carbohydrate-binding module
additional information
the encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module
additional information
homology model of the TLam structure, overview
additional information
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homology model of the TLam structure, overview