3.2.1.132: chitosanase
This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.
Word Map on EC 3.2.1.132
-
3.2.1.132
-
chitosans
-
chitooligosaccharides
-
deacetylation
-
oligosaccharide
-
glycoside
-
colloidal
-
chitinases
-
glcn
-
endo-type
-
biocontrol
-
paenibacillus
-
circulans
-
chitotetraose
-
chitosanolytic
-
d-glucosamine
-
food industry
-
chitinolytic
-
chitotriose
-
synthesis
-
biotechnology
-
glucanase
-
analysis
-
industry
-
pharmacology
-
coss
-
medicine
-
chitopentaose
-
chitooligomers
-
lichenan
-
microbacterium
-
agriculture
- 3.2.1.132
- chitosans
- chitooligosaccharides
-
deacetylation
- oligosaccharide
- glycoside
-
colloidal
- chitinases
-
glcn
-
endo-type
-
biocontrol
- paenibacillus
- circulans
- chitotetraose
-
chitosanolytic
- d-glucosamine
- food industry
-
chitinolytic
- chitotriose
- synthesis
- biotechnology
- glucanase
- analysis
- industry
- pharmacology
- coss
- medicine
- chitopentaose
-
chitooligomers
- lichenan
- microbacterium
- agriculture
Reaction
Synonyms
actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase
ECTree
3.2.1.132 chitosanaseAdvanced search results
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Substrates Products on EC 3.2.1.132 - chitosanase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(GlcN)7 + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
Pedobacter sp. PR-M6
-
-
-
-
?
4-methylumbelliferyl beta-chitotrioside + H2O
4-methylumbelliferone + chitotriose + chitobiose + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
-
-
-
-
?
aminoethyl chitosan + H2O
aminoethyl low molecular weight chitosan + ?
-
average molecular weight of aminoethyl low molecular weight chitosan is 8.89 kDa
-
-
?
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
Pedobacter sp. PR-M6
-
-
-
-
?
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
Pedobacter sp. PR-M6
-
-
-
-
?
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
(GlcN)6 is mainly hydrolyzed into two molecules of (GlcN)3, accompanied by a very small proportion of (GlcN)2 and (GlcN)4
-
-
?
carboxymethyl chitin + H2O
?
-
isoform A shows 41.8% activity and isoform B 17.2% activity compared to chitosan
-
-
?
carboxymethyl chitosan + H2O
carboxymethyl low molecular weight chitosan + ?
-
average molecular weight of carboxymethyl low molecular weight chitosan is 11.2 kDa
-
-
?
chitohexaose + H2O
chitobiose + chitotriose + chitotetraose
-
-
mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme
-
?
chitohexaose + H2O
chitotriose + chitobiose + chitotetraose
-
-
triglucosamine is the main product
-
?
chitosan + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN + ?
colloidal chitosan, endotype cleavage pattern and hydrolysis of chitosan to yield (GlcN)2 as the major product
-
-
?
chitosan + H2O
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN, (GlcN)4 and (GlcN)5 are the major products
-
-
?
chitosan + H2O
chitobiose + chitooligosaccharides
-
chitodisaccharide is the main product (69.9% of the total product)
-
-
?
chitosan + H2O
chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose
chitosan + H2O
chitotriose + chitotetraose + chitopentaose
-
-
major hydrolytic products
-
?
chitosan + H2O
chitotriose + chitotetraose + chitopentaose + chitohexaose
the main products of chitosan hydrolyzed by recombinant chitosanase are (GlcN)3-6
-
-
?
chitosan + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
chitosan + H2O
glucosamine oligomer + N-acetyl-glucosamine oligomer
-
-
hydrolysates are oligomers with one to four glucosamine residues and some oligomers with longer chain length
-
?
chitosan + H2O
low molecular weight chitosan polymers + chitosan oligomers
Ficus sp.
-
most effectively hydrolyzes chitosan polymers that are 52-70% deacetylated
-
-
?
chitosan hexasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
ethylene glycol chitosan + H2O
ethylene glycol low molecular weight chitosan + ?
-
average molecular weight of ethylene glycol low molecular weight chitosan is 11.2 kDa
-
-
?
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN
-
best substrate
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) is the main product
-
?
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + H2O
?
-
-
-
-
?
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + D-glucosamine
-
-
-
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN
-
-
-
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
-
-
-
-
?
N-acetylated chitosan + H2O
?
activity is ca. 3fold less than toward chitosan
-
-
?
water-soluble chitosan + H2O
chitosan oligosaccharides
-
substrate 40-50% deacetylated, 7.6% of the activity with colloidal chitosan
-
-
?
beta-chitin + H2O
?
-
85% activity with chitin (beta-type) compared to chitosan (95% DD)
-
-
?
beta-chitin + H2O
?
Serratia marcescens TKU019
-
85% activity with chitin (beta-type) compared to chitosan (95% DD)
-
-
?
carboxylmethyl cellulose + H2O
?
-
61.9% activity compared to chitosan
-
-
?
carboxymethyl cellulose + H2O
?
-
55% activity compared to 50% deacetylated chitosan
-
-
?
carboxymethyl cellulose + H2O
?
-
5.3% of the activity with soluble chitosan
-
-
?
carboxymethyl cellulose + H2O
?
-
5.3% of the activity with soluble chitosan
-
-
?
carboxymethylcellulose + H2O
?
58% activity compared to chitosan
-
-
?
carboxymethylcellulose + H2O
?
58% activity compared to chitosan
-
-
?
chitin + H2O
?
-
isoform A shows 7.8% activity and isoform B 10.8% activity compared to chitosan
-
-
?
chitoheptaose + H2O
?
23.8% of the activity with chitosan
-
-
?
chitoheptaose + H2O
?
Bacillus sp. (in: Bacteria) KCTC 0377BP
23.8% of the activity with chitosan
-
-
?
chitohexaose + H2O
?
20.0% of the activity with chitosan
-
-
?
chitohexaose + H2O
?
-
ChiN hydrolyzes oligomers larger than chitopentaose
-
-
?
chitohexaose + H2O
?
-
ChiN hydrolyzes oligomers larger than chitopentaose
-
-
?
chitopentaose + H2O
?
16.8% of the activity with chitosan
-
-
?
chitopentaose + H2O
?
Bacillus sp. (in: Bacteria) KCTC 0377BP
16.8% of the activity with chitosan
-
-
?
chitosan + H2O
?
-
70-100% deacetylated chitosan, the activity of AsChi increases with the degree of deacetylation of chitosan. AsChi probably hydrolyzes chitosan in an endo-type fashion
-
-
?
chitosan + H2O
?
-
70-100% deacetylated chitosan, the activity of AsChi increases with the degree of deacetylation of chitosan. AsChi probably hydrolyzes chitosan in an endo-type fashion
-
-
?
chitosan + H2O
?
exhibits antifungal activity against Rhizopus oryzae IAM6252, which is known to produce chitosan probably as a cell wall component. Glu22 acts as an active center in the CtoA protein
-
-
?
chitosan + H2O
?
-
100% activity with 50% deacetylated chitosan, 89% activity with 70% deacetylated chitosan, 91% activity with 98% deacetylated chitosan
-
-
?
chitosan + H2O
?
-
the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan
-
-
?
chitosan + H2O
?
-
the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan
-
-
?
chitosan + H2O
?
-
100% activity with chitosan of 60% degrees of deacetylation
-
-
?
chitosan + H2O
?
-
100% activity with chitosan of 60% degrees of deacetylation
-
-
?
chitosan + H2O
?
-
-
-
-
?
chitosan + H2O
?
-
-
-
-
?
chitosan + H2O
?
the mutant enzymes E309R/N319E and N308V/E309R/N319E can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wild-type enzyme
-
-
?
chitosan + H2O
?
-
a chitosanase classification system is suggested which is based on specificities and preferences of subsites (-2) to (+2)
-
-
?
chitosan + H2O
?
-
the enzyme shows activity toward chitosan polymers which exhibit various degrees of deacetylation (21-94%). It hydrolyzes 70-84% deacetylated chitosan polymers most effectively. Substrate specificity analysis indicates that the enzyme catalyzes the hydrolysis of chitin and chitosan polymers and their derivatives
-
-
?
chitosan + H2O
?
-
Chitosan, or beta-1,4-linked glucosamine, is a deacetylated derivative of chitin. The chitosanase is specific for chitosan as substrate
-
-
?
chitosan + H2O
?
-
the chitosanase is specific for chitosan as substrate
-
-
?
chitosan + H2O
?
-
Chitosan, or beta-1,4-linked glucosamine, is a deacetylated derivative of chitin. The chitosanase is specific for chitosan as substrate
-
-
?
chitosan + H2O
?
-
the chitosanase is specific for chitosan as substrate
-
-
?
chitosan + H2O
?
-
activity increases with the degree of deacetylation of chitosan
-
-
?
chitosan + H2O
?
-
activity increases with the degree of deacetylation of chitosan
-
-
?
chitosan + H2O
?
-
the chitosanase is an endo-type enzyme, the preferred substrates of chitosanase are chitosan preparations with a high degree of deacetylation, substrate is chitosan 66-97% deacetylated
-
-
?
chitosan + H2O
?
a chitosanase classification system is suggested which is based on specificities and preferences of subsites (-2) to (+2)
-
-
?
chitosan + H2O
?
Niallia circulans MH-K1
a chitosanase classification system is suggested which is based on specificities and preferences of subsites (-2) to (+2)
-
-
?
chitosan + H2O
?
-
chitosanase is a glycosyl hydrolase that endolytically hydrolyzes beta-1,4-linkages between D -glucosamine residues in a partially acetylated chitosan
-
-
?
chitosan + H2O
?
-
excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%)
-
-
?
chitosan + H2O
?
Paenibacillus mucilaginosus TKU032
-
excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%)
-
-
?
chitosan + H2O
?
the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction
-
-
?
chitosan + H2O
?
the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction
-
-
?
chitosan + H2O
?
-
activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan
-
-
?
chitosan + H2O
?
substrate recognition mechanismis for non-processive chitosanase is decribed
-
-
?
chitosan + H2O
?
-
activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan
-
-
?
chitosan + H2O
?
-
activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation
-
-
?
chitosan + H2O
?
-
100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation)
-
-
?
chitosan + H2O
?
-
activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation
-
-
?
chitosan + H2O
?
Serratia marcescens TKU019
-
100% activity with chitosan (95% degree of deacetylation), 26% activity with chitosan (85% degree of deacetylation)
-
-
?
chitosan + H2O
?
-
chitosan of approximately 100 kDa and degree of deacetylation 96%
-
-
?
chitosan + H2O
?
the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%)
-
-
?
chitosan + H2O
?
-
chitosan of approximately 100 kDa and degree of deacetylation 96%
-
-
?
chitosan + H2O
?
a chitosanase classification system is suggested which is based on specificities and preferences of subsites (-2) to (+2)
-
-
?
chitosan + H2O
?
chitosan 10B. The enzyme has both cellulase activity and chitosanase activity
-
-
?
chitosan + H2O
chitobiose + chitotriose + chitotetraose
-
activity increases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 62-100% degree of deacetylation. Hydrolysis of chitosan with 99% degree of deacetylation by ChiN releases chitobiose, chitotriose and chitotetraose
-
-
?
chitosan + H2O
chitobiose + chitotriose + chitotetraose
-
activity increases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 62-100% degree of deacetylation. Hydrolysis of chitosan with 99% degree of deacetylation by ChiN releases chitobiose, chitotriose and chitotetraose
-
-
?
chitosan + H2O
chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose
the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction
-
-
?
chitosan + H2O
chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose
the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction
-
-
?
chitosan + H2O
chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose
-
high activity on 90% deacetylated colloidal chitosan
chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased
-
?
chitosan + H2O
chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose
Bacillus sp. (in: Bacteria) MET 1299
-
high activity on 90% deacetylated colloidal chitosan
chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased
-
?
chitosan + H2O
chitopentaose + chitohexaose
-
85% deacetylated soluble chitosan is the most susceptible substrate
products after 24 h incunation at 70°C
-
?
chitosan + H2O
chitopentaose + chitohexaose
-
85% deacetylated soluble chitosan is the most susceptible substrate
products after 24 h incunation at 70°C
-
?
chitosan + H2O
chitopentaose + chitotetraose + chitotriose
about 99% deacetylated substrate
-
-
?
chitosan + H2O
chitopentaose + chitotetraose + chitotriose
about 99% deacetylated substrate
-
-
?
chitosan + H2O
chitosan oligomers
-
chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified
-
?
chitosan + H2O
chitosan oligomers
-
chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified
-
?
chitosan + H2O
chitosan oligosaccharides
the enzyme prefers to hydrolyze chitosan with high degree of deacetylation. The main products of chitiosan (92% deacetylation) are chitosan oligosaccharides with degree of polymerization (DP) ranging from 2 to 6. No activity with colloidal chitin, carboxymethylcellulose, microcrystalline cellulose, glucosan
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
-
chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers
-
?
chitosan + H2O
chitosan oligosaccharides
-
-
the enzyme produces various chitooligomers with different degrees of polymerisation from 3 to 8
-
?
chitosan + H2O
chitosan oligosaccharides
-
-
chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers
-
?
chitosan + H2O
chitosan oligosaccharides
-
-
the enzyme produces various chitooligomers with different degrees of polymerisation from 3 to 8
-
?
chitosan + H2O
chitosan oligosaccharides
activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
1 g of enzyme can hydrolyze about 100 kg of chitosan
-
-
?
chitosan + H2O
chitosan oligosaccharides
Bacillus sp. (in: Bacteria) KCTC 0377BP
activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate with 85% deacetylation
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate with 85% deacetylation
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate colloidal chitosan, 85% deacetylated
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate colloidal chitosan, 85% deacetylated
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate with average molecular weight of 220 kDA, 85% deacetylated
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
-
products are a variety of oligomers
-
?
chitosan + H2O
chitosan oligosaccharides
-
substrate with average molecular weight of 220 kDA, 85% deacetylated
-
-
?
chitosan + H2O
chitosan-oligosaccharides
85% deacetylated chitin, transformants show a significant increase in chitosanase production by 2.1fold than control
-
-
?
chitosan + H2O
chitosan-oligosaccharides
catalyzes the hydrolysis of beta-1,4-glycosidic links
-
-
?
chitosan + H2O
chitotriose + chitotetraose
-
-
-
?
chitosan + H2O
D-glucosamine + ?
-
chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B
-
-
?
chitosan + H2O
D-glucosamine + ?
-
hydrolysis of chitosan hexamer, chitosan substrates are 55-80% deacetylated
dimers and trimers
-
?
chitosan + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type
-
-
?
chitosan + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
Niallia circulans MH-K1
chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type
-
-
?
chitosan heptasaccharide + H2O
chitosan pentasaccharide + chitosan disaccharide
-
-
-
-
?
chitosan heptasaccharide + H2O
chitosan pentasaccharide + chitosan disaccharide
-
-
-
-
?
chitosan heptasaccharide + H2O
chitosan tetrasaccharide + chitosan trisaccharide
-
-
-
-
?
chitosan heptasaccharide + H2O
chitosan tetrasaccharide + chitosan trisaccharide
-
-
-
-
?
chitosan hexasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
-
-
-
-
?
chitosan hexasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
-
-
-
-
?
chitosan hexasaccharide + H2O
chitosan tetrasaccharide + chitosan disaccharide
-
-
-
-
?
chitosan hexasaccharide + H2O
chitosan tetrasaccharide + chitosan disaccharide
-
-
-
-
?
chitosan pentasaccharide + H2O
?
-
92% deacetylated chitosan, development of a Remazol Brilliant Blue R dye-coupled, quantitative enzyme assay method, overview
-
-
?
chitosan pentasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide
-
-
-
-
?
chitosan pentasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide
-
hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3
-
-
?
chitosan pentasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide
-
hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3
-
-
?
chitosan pentasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide
hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3
-
-
?
chitotetraose + H2O
?
15.0% of the activity with chitosan
-
-
?
chitotetraose + H2O
?
Bacillus sp. (in: Bacteria) KCTC 0377BP
15.0% of the activity with chitosan
-
-
?
colloidal chitin + H2O
?
-
52% activity compared to 50% deacetylated chitosan
-
-
?
colloidal chitin + H2O
?
-
5.1% of the activity with colloidal chitosan
-
-
?
colloidal chitosan + H2O
chitobiose + chitotriose + chitotetraose
85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan
-
-
?
colloidal chitosan + H2O
chitobiose + chitotriose + chitotetraose
85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan
-
-
?
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
?
-
-
-
-
?
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
?
-
-
-
-
?
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
?
-
-
-
-
?
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
?
-
-
-
-
?
glycol chitin + H2O
?
-
isoform A shows 29.7% activity and isoform B 14.1% activity compared to chitosan
-
-
?
glycol chitin + H2O
?
-
11% activity compared to colloidal chitosan
-
-
?
glycol chitin + H2O
?
-
11% activity compared to colloidal chitosan
-
-
?
glycol chitosan + H2O
?
weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan
-
-
?
glycol chitosan + H2O
?
weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan
-
-
?
glycol chitosan + H2O
?
activity is ca. 4fold higher than toward chitosan
-
-
?
glycol chitosan + H2O
?
-
hydrolysis of glycol chitosan with release of glucosamine
-
-
?
glycol chitosan + H2O
?
-
12% activity compared to colloidal chitosan
-
-
?
glycol chitosan + H2O
?
-
12% activity compared to colloidal chitosan
-
-
?
glycol chitosan + H2O
glycol chitosan oligosaccharides
-
substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan
-
-
?
glycol chitosan + H2O
glycol chitosan oligosaccharides
-
substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan
-
-
?
methylcellulose + H2O
?
14% activity compared to chitosan
-
-
?
soluble chitosan + H2O
?
-
activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 82-100% degree of deacetylation.
-
-
?
soluble chitosan + H2O
?
Bacillus sp. (in: Bacteria) TKU004
-
activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 82-100% degree of deacetylation.
-
-
?
soluble chitosan + H2O
?
85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan
-
-
?
soluble chitosan + H2O
?
85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan
-
-
?
chitohexaose + H2O
additional information
-
-
chitosanase I
-
?
chitopentaose + H2O
additional information
-
-
chitosanase I
-
?
chitopentaose + H2O
additional information
-
-
chitosanase I
-
?
chitosan + H2O
additional information
-
alpine bacterium
-
most effective with 70% deacetylated chitosan as substrate
-
-
?
chitosan + H2O
additional information
-
alpine bacterium YSK-28
-
most effective with 70% deacetylated chitosan as substrate
-
-
?
chitosan + H2O
additional information
-
-
enzyme is capable of cleaving between glucosamine and glucosamine or N-acetylglucosamine, but not cleaving between N-acetylglucosamine and glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
the smallest of the substrates is a tetramer of glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
most susceptible substrates are: chitohexaose and chitoheptaose
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
most susceptible substrate is: 100% deacetylated chitosan
-
-
?
chitosan + H2O
additional information
-
-
deacetylation degree from 70% to 100%
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: chitosan extracted from Mucor rouxii cell walls
mixture of dimer, trimer and higher molecular weight oligomers of glucosamine
?
chitosan + H2O
additional information
-
-
-
chitosanase I produced glucosamine, chitosanase II produced chitooligosaccharides
?
chitosan + H2O
additional information
-
-
-
chitosanase I produced glucosamine, chitosanase II produced chitooligosaccharides
?
chitosan + H2O
additional information
-
-
enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation
-
-
?
chitosan + H2O
additional information
-
-
enzyme I prefers chitosan with 30% acetylation, but enzyme II mostly effects chitosan without acetylation
-
-
?
chitosan + H2O
additional information
-
-
-
products from 100% deacetylated chitosan are chitobiose: 27.2%, chitotriose: 40.6% and chitotetraose: 32.2%
?
chitosan + H2O
additional information
-
-
-
products from 100% deacetylated chitosan are chitobiose: 27.2%, chitotriose: 40.6% and chitotetraose: 32.2%
?
chitosan + H2O
additional information
-
-
specificity
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
specificity
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
enzyme degrades soluble and colloidal chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: soluble chitosan
glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan
?
chitosan + H2O
additional information
-
-
enzyme is absolutely specific towards the GlcN-GlcN bonds in partially N-acetylated chitosan and at least three GlcN residues are necessary to hydrolyze chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan
?
chitosan + H2O
additional information
-
-
enzyme degrades soluble and colloidal chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
-
-
?
chitosan + H2O
additional information
-
-
enzyme is absolutely specific towards the GlcN-GlcN bonds in partially N-acetylated chitosan and at least three GlcN residues are necessary to hydrolyze chitosan
-
-
?
chitosan + H2O
additional information
-
-
enzyme degrades soluble and colloidal chitosan
-
-
?
chitosan + H2O
additional information
-
Bacillus sp. (in: Bacteria) No. 7-M
-
enzyme is absolutely specific towards the GlcN-GlcN bonds in partially N-acetylated chitosan and at least three GlcN residues are necessary to hydrolyze chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
-
-
?
chitosan + H2O
additional information
-
-
degrades 60-94% deacetylated chitosan most effectively
end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length
?
chitosan + H2O
additional information
-
-
degrades 60-94% deacetylated chitosan most effectively
end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length
?
chitosan + H2O
additional information
-
-
most effective with 70-100% deacetylated chitosan as substrate
-
-
?
chitosan + H2O
additional information
-
-
most effective with 70-100% deacetylated chitosan as substrate
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated
GlcNAc4-6 and colloidal chitin are hydrolyzed to GlcNAc2
?
chitosan + H2O
additional information
-
-
hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated
the main cleavage site with GlcNAc3-6 is the second linkage from the non-reducing end, based on the pattern of pNp-GlcNAc2-5
?
chitosan + H2O
additional information
-
-
hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated
colloidal chitosan is hydrolyzed to GlcNAc2 and to similar partially N-acetylated chitooligosaccharides
?
chitosan + H2O
additional information
-
-
deacetylation degree from 70% to 100%
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
-
-
?
chitosan + H2O
additional information
-
-
the smallest of the substrates is a tetramer of glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
substrates with less than 40% deacetylation are not affected by the enzyme
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
most susceptible substrate is: 80% deacetylated chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
Niallia circulans MH-K1
-
the smallest of the substrates is a tetramer of glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
Niallia circulans MH-K1
-
substrates with less than 40% deacetylation are not affected by the enzyme
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
Niallia circulans MH-K1
-
most susceptible substrate is: 80% deacetylated chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
enzyme A is highly specific for chitosan, enzyme B and C possess comparable specific activities towards chitosan, chitin and cellulose
glucosamine oligomers, predominantly dimers and trimers
?
chitosan + H2O
additional information
-
-
high substrate specificity for highly deacetylated chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
high substrate specificity for highly deacetylated chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
-
chitosan of an average MW 36000 is reduced by the enzymatic catalysis to nearly one-fourth this size
?
chitosan + H2O
additional information
-
-
-
chitosan of an average MW 36000 is reduced by the enzymatic catalysis to nearly one-fourth this size
?
chitosan + H2O
additional information
-
-
hydrolysis of: soluble chitosan
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: colloidal chitosan 80% deacetylated, not 100% deacetylated
-
-
?
chitosan + H2O
additional information
-
-
hydrolysis of: glycol chitosan
-
-
?
chitosan + H2O
additional information
-
-
-
diglucosamine and triglucosamine
?
chitosan + H2O
additional information
-
-
the smallest of the substrates is a tetramer of glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
-
-
-
diglucosamine and triglucosamine
?
chitosan + H2O
additional information
-
-
most effective with 68-88% deacetylated chitosan as substrate
-
-
?
chitosan + H2O
additional information
-
-
polymers with 30% to 60% acetylation
with 60% acetylated chitosan as substrate a dimer is also found
?
chitosan + H2O
additional information
-
-
polymers with 30% to 60% acetylation
major oligomeric product from 30% acetylated chitosan is a trimer
?
chitosan + H2O
additional information
-
-
polymers with 30% to 60% acetylation
the products depend on the degree of acetylation of the polymer
?
chitosan + H2O
additional information
-
-
polymers containing equal proportions of acetylated and non-acetylated sugars are optimal for chitosanase activity
with 60% acetylated chitosan as substrate a dimer is also found
?
chitosan + H2O
additional information
-
-
polymers containing equal proportions of acetylated and non-acetylated sugars are optimal for chitosanase activity
major oligomeric product from 30% acetylated chitosan is a trimer
?
chitosan + H2O
additional information
-
-
polymers containing equal proportions of acetylated and non-acetylated sugars are optimal for chitosanase activity
the products depend on the degree of acetylation of the polymer
?
additional information
?
-
-
no activity with 50% deacetylated chitosan, 19% deacetylated colloidal chitin, carboxymethylcellulose sodium, and carboxymethyl chitosan
-
-
?
additional information
?
-
-
no activity with 50% deacetylated chitosan, 19% deacetylated colloidal chitin, carboxymethylcellulose sodium, and carboxymethyl chitosan
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
-
no activity with glucosamine dimer and trimer
-
-
?
additional information
?
-
-
degrades glucosamine tetramer to dimer and pentamer and to dimer and trimer
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose
-
-
?
additional information
?
-
-
the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose
-
-
?
additional information
?
-
-
the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds
-
-
?
additional information
?
-
-
enzyme is able to catalyze the synthesis of small amounts of chitooctaose from a mixture of chitobiose to chitoheptaose oligomers, possible through transglycosylation. Pentamer and hexamer oligosaccharides are the main glycosyl acceptors
-
-
?
additional information
?
-
-
the chitosanase splits (GlcN)4GlcNOH into (GlcN)3 + (GlcN)1GlcNOH, and (GlcN)5GlcNOH into (GlcN)4 + (GlcN)1GlcNOH and (GlcN)3 + (GlcN)2GlcNOH. The heptamer (GlcN)6GlcNOH is split into (GlcN)1GlcNOH + (GlcN)5, thereafter hydrolyzed again into (GlcN)3 + (GlcN)2, or into (GlcN)4 + (GlcN)2GlcNOH, as well as (GlcN)3 + (GlcN)3GlcNOH, whereas (GlcN)1-3GlcNOH is not hydrolyzed. The monomers GlcN and GlcNOH are never detected from the enzyme reaction. Substrate specificity and product analysis, overview
-
-
?
additional information
?
-
-
the chitosanase splits (GlcN)4GlcNOH into (GlcN)3 + (GlcN)1GlcNOH, and (GlcN)5GlcNOH into (GlcN)4 + (GlcN)1GlcNOH and (GlcN)3 + (GlcN)2GlcNOH. The heptamer (GlcN)6GlcNOH is split into (GlcN)1GlcNOH + (GlcN)5, thereafter hydrolyzed again into (GlcN)3 + (GlcN)2, or into (GlcN)4 + (GlcN)2GlcNOH, as well as (GlcN)3 + (GlcN)3GlcNOH, whereas (GlcN)1-3GlcNOH is not hydrolyzed. The monomers GlcN and GlcNOH are never detected from the enzyme reaction. Substrate specificity and product analysis, overview
-
-
?
additional information
?
-
-
enzyme is able to catalyze the synthesis of small amounts of chitooctaose from a mixture of chitobiose to chitoheptaose oligomers, possible through transglycosylation. Pentamer and hexamer oligosaccharides are the main glycosyl acceptors
-
-
?
additional information
?
-
-
chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus
-
-
?
additional information
?
-
-
no substrates: colloidal and glycol chitin
-
-
?
additional information
?
-
-
chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus
-
-
?
additional information
?
-
-
no substrates: colloidal and glycol chitin
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
-
no activity with glycol chitin
-
-
?
additional information
?
-
-
no activity with powdered chitosan
-
-
?
additional information
?
-
-
no activity with methylcellulose
-
-
?
additional information
?
-
-
no activity with colloidal chitin
-
-
?
additional information
?
-
-
the enzyme is constitutively produced
-
-
?
additional information
?
-
-
no activity on colloidal chitin
-
-
?
additional information
?
-
no hydrolysis of chitobiose and chitotriose
-
-
?
additional information
?
-
-
no hydrolysis of chitobiose and chitotriose
-
-
?
additional information
?
-
-
the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose
-
-
?
additional information
?
-
-
chitosanase has antioxidant activity. Squid pen powder is a carbon/nitrogen source for chitosanase production. Autoclave treatment of squid pen powder for 45 min remarkably promotes enzyme productivity, production of chitosanase is highest when 3% squid pen powder is used. No acitivity with chitosan (80% degree of deacetylation), chitosan (73% degree of deacetylation), chitosan (60% degree of deacetylation) and chitin (alpha-type)
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
-
no activity with glycol chitin
-
-
?
additional information
?
-
-
no activity with powdered chitosan
-
-
?
additional information
?
-
-
no activity with cellulose
-
-
?
additional information
?
-
-
no activity with methylcellulose
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) KCTC 0377BP
no hydrolysis of chitobiose and chitotriose
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) MET 1299
-
the enzyme is constitutively produced
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) MET 1299
-
no activity on colloidal chitin
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) No. 7-M
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) No. 7-M
-
no activity with glycol chitin
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) No. 7-M
-
no activity with powdered chitosan
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) No. 7-M
-
no activity with cellulose
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) No. 7-M
-
no activity with methylcellulose
-
-
?
additional information
?
-
-
no activity with chitin (i.e. 100% acetylated chitosan)
-
-
?
additional information
?
-
-
the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) TKU004
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chitosanase has antioxidant activity. Squid pen powder is a carbon/nitrogen source for chitosanase production. Autoclave treatment of squid pen powder for 45 min remarkably promotes enzyme productivity, production of chitosanase is highest when 3% squid pen powder is used. No acitivity with chitosan (80% degree of deacetylation), chitosan (73% degree of deacetylation), chitosan (60% degree of deacetylation) and chitin (alpha-type)
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additional information
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the enzyme does not hydrolyze glycolchitosan, chitin, carboxymethyl cellulose, barley beta-glucan or phosphoric acid swollen cellulose (0.2% each)
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additional information
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the enzyme does not hydrolyze glycolchitosan, chitin, carboxymethyl cellulose, barley beta-glucan or phosphoric acid swollen cellulose (0.2% each)
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additional information
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does not hydrolyze colloidal chitin and carboxylmethyl cellulose
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additional information
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does not hydrolyze colloidal chitin and carboxylmethyl cellulose
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additional information
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chitosanases hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues
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additional information
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the enzyme is specific for chitosan as substrate, no activity with colloidal chitin, insoluble cellulose, CM cellulose, beta-1,3-glucan, xylan, and arabinoxylan
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additional information
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at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan
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additional information
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at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan
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additional information
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The enzyme might be identical with a lipase that co-purifies with the chitosanase. No activity for colloidal chitin, Na-CMC and starch
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additional information
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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Niallia circulans MH-K1
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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the enzyme is not active toward colloidal chitin or carboxymethyl cellulose
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additional information
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the enzyme is not active toward colloidal chitin or carboxymethyl cellulose
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additional information
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bacterial chitosanase of the GH-8 group possessing additional glucanase activity
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additional information
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the chitosanase from Paenibacillus fukuinensis exhibits both chitosanase and beta-1,4 glucanase activities. Glu302 is a proton acceptor for chitosanase activity, and Asn312 also participates in the hydrolysis of chitosan and cellulose
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additional information
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the chitosanase from Paenibacillus fukuinensis exhibits both chitosanase and beta-1,4 glucanase activities. Glu302 is a proton acceptor for chitosanase activity, and Asn312 also participates in the hydrolysis of chitosan and cellulose
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additional information
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no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide
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additional information
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Paenibacillus mucilaginosus TKU032
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no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide
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additional information
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does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan
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additional information
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does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan
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additional information
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no substrate: carboxymethyl chitosan
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additional information
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no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan
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additional information
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no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan
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additional information
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no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity
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additional information
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the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin
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additional information
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no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity
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additional information
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Serratia marcescens TKU019
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the enzyme shows no activity with chitosan (73% and 60% degrees of deacetylation), glycols chitosan, and alpha-chitin
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additional information
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comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis. The preferred cleavage site of enzyme CSN1 is between GlcN and GlcNAc
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additional information
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the enzyme cannot hydrolyze colloidal chitin, carboxylmethyl cellulose, diglucosamine, triglucosamine, or tetraglucosamine
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additional information
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the enzyme cannot hydrolyze colloidal chitin, carboxylmethyl cellulose, diglucosamine, triglucosamine, or tetraglucosamine
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additional information
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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chitosan crosslinked with trimellitic anhydride, diisocyanatohexane, and dibromodecane show the same hydrolytic behaviour as uncrosslinked chitosan. Crosslinked chitosan species which are complexed with metals exhibits a significantly reduced extent of hydrolysis
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additional information
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the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed
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additional information
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comparison of activities on chitosan by cellobiohydrolases, chitosanases, and lysozyme, oligomer pattern, overview. The different enzymes produce chito-oligosaccharides (COSs) with varying acetylation, NMR spectrometric analysis. The preferred cleavage site of enzyme CSN2 is between GlcN(Ac) and GlcN(Ac)
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additional information
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does not hydrolyze GlcNbeta(1-4)GlcN or GlcNbeta(1-4)GlcNbeta(1-4)GlcN
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additional information
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed
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additional information
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the enzyme is not able to degrade chitin
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additional information
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the enzyme is not able to degrade chitin
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additional information
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no activity with chitin (i.e. 100% acetylated chitosan)
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additional information
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slow cleavage of: pentamer of N-acetylglucosamine, glucosamine oligomers
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additional information
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no activity with trimers or tetramers of N-acetylglucosamine, carboxymethylcellulose, dextran, galactan, polygalacturonic acid, laminaran, mucoran, pectin, pullulan, salicin, xylan, 4-nitrophenyl-beta-D-acetylglucosamine, 4-nitrophenyl-beta-D-glucosamine
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additional information
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enzyme cleaves both glucosamine-glucosamine and N-acetylglucosamine-glucosamine linkages of chitosan. Enzyme additionally has cellulase activity for carboxymethylcellulose
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additional information
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catalytic residues Glu121 and Glu141 are important for the antifungal effect of the cho product
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