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Literature summary for 3.2.1.132 extracted from
- Identification, characterization, and regulation of a novel antifungal chitosanase gene (cho) in Anabaena spp. (2010), Appl. Environ. Microbiol., 76, 2769-2777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cho, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Trichormus fertilissimus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichormus fertilissimus | C9D7S2 | RPAN1, gene cho | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan pentasaccharide + H2O | hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 | Trichormus fertilissimus | chitosan disaccharide + chitosan trisaccharide | - |
? |  |
| additional information | catalytic residues Glu121 and Glu141 are important for the antifungal effect of the cho product | Trichormus fertilissimus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycoside hydrolase 3, GH3-like family of Anabaena variabilis ATCC 29413 | Trichormus fertilissimus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Trichormus fertilissimus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Trichormus fertilissimus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Trichormus fertilissimus | the enzyme's chitosanase and antifungal activities are induced significantly under of growth-limiting conditions of 8 of light and 16 h of darkness | up |
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Release 2023.1 (January 2023)
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