Information on EC 3.2.1.132 - chitosanase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.132
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RECOMMENDED NAME
GeneOntology No.
chitosanase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
chitosan N-acetylglucosaminohydrolase
A whole spectrum of chitosanases are now known (for more details, see {http://rbrzezinski.recherche.usherbrooke.ca/::http://rbrzezinski.recherche.usherbrooke.ca/}). They can hydrolyse various types of links in chitosan. The only constant property is the endohydrolysis of GlcN-GlcN links, which is common to all known chitosanases. One known chitosanase is limited to this link recognition [4], while the majority can also recognize GlcN-GlcNAc links or GlcNAc-GlcN links but not both. They also do not recognize GlcNAc-GlcNAc links in partly acetylated chitosan.
CAS REGISTRY NUMBER
COMMENTARY hide
51570-20-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
C-17
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Manually annotated by BRENDA team
C-17
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Manually annotated by BRENDA team
strain HG08
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Manually annotated by BRENDA team
strain HG08
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-
Manually annotated by BRENDA team
alpine bacterium
strain YSK-28
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Manually annotated by BRENDA team
alpine bacterium YSK-28
strain YSK-28
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Manually annotated by BRENDA team
CsO-2
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Manually annotated by BRENDA team
Amycolatopsis sp.CsO-2
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
KB-1
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Manually annotated by BRENDA team
KH-94
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Manually annotated by BRENDA team
synonym Aspergillus fumigatus
UniProt
Manually annotated by BRENDA team
IAM2660
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-
Manually annotated by BRENDA team
IAM2660
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Bacillus cereus NTU-FC-4
strain NTU-FC-4
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Manually annotated by BRENDA team
S1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain MB-2
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Manually annotated by BRENDA team
Bn-262
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Manually annotated by BRENDA team
Bn-262
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Manually annotated by BRENDA team
739
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Manually annotated by BRENDA team
CK4
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Manually annotated by BRENDA team
Bacillus sp. HW-002
HW-002
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Manually annotated by BRENDA team
strain K17
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Manually annotated by BRENDA team
strain KCTC 0377BP
SwissProt
Manually annotated by BRENDA team
Bacillus sp. MET 1299
strain MET 1299
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Manually annotated by BRENDA team
Bacillus sp. No. 7-M
No. 7-M
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Manually annotated by BRENDA team
P16
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Manually annotated by BRENDA team
PI-7S
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Manually annotated by BRENDA team
strain S65
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Manually annotated by BRENDA team
Bacillus sp. TKU004
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GM9804
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Manually annotated by BRENDA team
IMR-NK1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain CHB101
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Manually annotated by BRENDA team
strain CHB101
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Ficus sp.
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Manually annotated by BRENDA team
strain 0114, gene csn1
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Manually annotated by BRENDA team
Gongronella sp.
Gongronella sp. JG
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Janthinobacterium sp.
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Manually annotated by BRENDA team
isolated from a fresh water lake close to Kangerlussuaq in West Greenland
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Manually annotated by BRENDA team
strain IB-9374, isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
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Manually annotated by BRENDA team
strain IB-9374, isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
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Manually annotated by BRENDA team
Macrotermes estherae
workers, soldiers, males, winged females
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Manually annotated by BRENDA team
strain QU01, chitosanase ChiN; strain QU01, chitosanase ChiX
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Manually annotated by BRENDA team
strain 3001
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Manually annotated by BRENDA team
strain 3001
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
chitosanase preprotein
UniProt
Manually annotated by BRENDA team
chitosanase preprotein
UniProt
Manually annotated by BRENDA team
strain ZD-Z1, isoform ChA
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Manually annotated by BRENDA team
strain ZD-Z1, isoform ChA
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
H-14
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Rhodosporidium toruloides
Rhodosporidium toruloides CFR-1
CFR-1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
roots colonized by Glomus mosseae or Glomus intraradices
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
HUT6037
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 174
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Manually annotated by BRENDA team
Streptomyces sp. No. 6
No. 6
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
isolate GB2 from Indralaya Swamp South Sumatera
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-chitotrioside + H2O
4-methylumbelliferone + chitotriose + chitobiose + 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
show the reaction diagram
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?
acetylated chitosan + H2O
?
show the reaction diagram
alginate + H2O
?
show the reaction diagram
alpha-chitin + H2O
?
show the reaction diagram
avicel + H2O
?
show the reaction diagram
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63% activity compared to 50% deacetylated chitosan
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?
beta-chitin + H2O
?
show the reaction diagram
beta-glucan + H2O
?
show the reaction diagram
carboxylmethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl chitin + H2O
?
show the reaction diagram
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isoform A shows 41.8% activity and isoform B 17.2% activity compared to chitosan
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?
carboxymethylcellulose + H2O
?
show the reaction diagram
carboxymethylchitosan + H2O
?
show the reaction diagram
chitin (beta-type) + H2O
?
show the reaction diagram
chitin + H2O
?
show the reaction diagram
chitobiose + H2O
glucosamine
show the reaction diagram
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chitosanase II
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?
chitoheptaose + H2O
?
show the reaction diagram
chitohexaose + H2O
?
show the reaction diagram
chitohexaose + H2O
chitobiose + chitotriose + chitotetraose
show the reaction diagram
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mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme
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?
chitopentaose + H2O
?
show the reaction diagram
chitosan + H2O
?
show the reaction diagram
chitosan + H2O
chitobiose + chitotriose + chitotetraose
show the reaction diagram
chitosan + H2O
chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose
show the reaction diagram
chitosan + H2O
chitopentaose + chitohexaose
show the reaction diagram
chitosan + H2O
chitopentaose + chitotetraose + chitotriose
show the reaction diagram
chitosan + H2O
chitosan oligomers
show the reaction diagram
chitosan + H2O
chitosan oligosaccharides
show the reaction diagram
chitosan + H2O
chitosan-oligosaccharides
show the reaction diagram
chitosan + H2O
chitotriose + chitotetraose
show the reaction diagram
chitosan + H2O
chitotriose + chitotetraose + chitopentaose
show the reaction diagram
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major hydrolytic products
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?
chitosan + H2O
D-glucosamine + ?
show the reaction diagram
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chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B
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?
chitosan + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
show the reaction diagram
chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type
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?
chitosan + H2O
glucosamine
show the reaction diagram
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hydrolysis of chitosan hexamer, chitosan substrates are 55-80% deacetylated
dimers and trimers
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?
chitosan + H2O
glucosamine oligomer + N-acetyl-glucosamine oligomer
show the reaction diagram
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hydrolysates are oligomers with one to four glucosamine residues and some oligomers with longer chain length
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?
chitosan + H2O
low molecular weight chitosan polymers + chitosan oligomers
show the reaction diagram
Ficus sp.
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most effectively hydrolyzes chitosan polymers that are 52-70% deacetylated
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?
chitosan heptasaccharide + H2O
chitosan pentasaccharide + chitosan disaccharide
show the reaction diagram
chitosan heptasaccharide + H2O
chitosan tetrasaccharide + chitosan trisaccharide
show the reaction diagram
chitosan hexasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
show the reaction diagram
chitosan hexasaccharide + H2O
chitosan tetrasaccharide + chitosan disaccharide
show the reaction diagram
chitosan hexasaccharide + H2O
chitosan trisaccharide
show the reaction diagram
chitosan pentasaccharide + H2O
?
show the reaction diagram
chitosan pentasaccharide + H2O
chitosan disaccharide + chitosan trisaccharide
show the reaction diagram
chitotetraose + H2O
?
show the reaction diagram
chitotetraose + H2O
chitobiose
show the reaction diagram
chitotriose + H2O
?
show the reaction diagram
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?
colloidal chitin + H2O
?
show the reaction diagram
colloidal chitosan + H2O
?
show the reaction diagram
colloidal chitosan + H2O
chitobiose + chitotriose + chitotetraose
show the reaction diagram
fucoidan + H2O
?
show the reaction diagram
Glc-beta-(1->4)-Glc-beta-(1->4)-beta-(1->4)-GlcNAc-GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) + H2O
?
show the reaction diagram
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-beta-(1->4)-GlcNAc + H2O
?
show the reaction diagram
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
?
show the reaction diagram
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?
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
?
show the reaction diagram
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?
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) + H2O
?
show the reaction diagram
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-beta-(1->4)-GlcNAc + H2O
?
show the reaction diagram
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?
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) + H2O
triglucosamine + diglucosamine + tetraglucosamine
show the reaction diagram
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triglucosamine is the main product
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?
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
?
show the reaction diagram
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) + Glc-beta-(1->4)-Glc-beta-(1->4) + GlcN-beta-(1->4)-GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)
show the reaction diagram
-
best substrate
GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) is the main product
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + D-glucosamine
show the reaction diagram
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?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN
show the reaction diagram
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?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
show the reaction diagram
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?
glycol chitin + H2O
?
show the reaction diagram
glycol chitosan + H2O
?
show the reaction diagram
glycol chitosan + H2O
glycol chitosan oligosaccharides
show the reaction diagram
hexa-N-acetylchitohexaose + H2O
?
show the reaction diagram
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-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
methylcellulose + H2O
?
show the reaction diagram
N,N'',N'''-triacetylchitotriose + H2O
?
show the reaction diagram
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-
-
-
?
N-acetyl-chitooligosaccharides + H2O
?
show the reaction diagram
-
-
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?
N-acetylated chitosan + H2O
?
show the reaction diagram
activity is ca. 3fold less than toward chitosan
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?
shrimp-shell powder + H2O
?
show the reaction diagram
soluble chitosan + H2O
?
show the reaction diagram
sulfate chitin + H2O
?
show the reaction diagram
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9.6% activity compared to chitosan
-
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?
water-soluble chitosan + H2O
chitosan oligosaccharides
show the reaction diagram
-
substrate 40-50% deacetylated, 7.6% of the activity with colloidal chitosan
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?
chitohexaose + H2O
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chitosan + H2O
?
show the reaction diagram
chitosan + H2O
chitosan oligomers
show the reaction diagram
chitosan + H2O
chitosan oligosaccharides
show the reaction diagram
chitosan + H2O
D-glucosamine + ?
show the reaction diagram
-
chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B
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?
chitosan pentasaccharide + H2O
?
show the reaction diagram
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-
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?
additional information
?
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