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Literature summary for 3.2.1.132 extracted from

  • Ogura, J.; Toyoda, A.; Kurosawa, T.; Chong, A.L.; Chohnan, S.; Masaki, T.
    Purification, characterization, and gene analysis of cellulase (Cel8A) from Lysobacter sp. IB-9374 (2006), Biosci. Biotechnol. Biochem., 70, 2420-2428.
    View publication on PubMed
Search for more literature for 3.2.1.132

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Lysobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.
41241
-
 x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.

Organism

Organism UniProt Comment Textmining
Lysobacter sp.
-
 isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
-
Lysobacter sp. IB-9374
-
 isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a 33-amino acid, N-terminal signal peptide and a mature enzyme Lysobacter sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Lysobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Lysobacter sp.
-

Storage Stability

Storage Stability Organism
4°C, pH 6.5, stable for at least 24 h Lysobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan Lysobacter sp. chitosan oligosaccharides
-
 ?
chitosan + H2O substrate colloidal chitosan, 85% deacetylated Lysobacter sp. chitosan oligosaccharides
-
 ?
chitosan + H2O substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan Lysobacter sp. IB-9374 chitosan oligosaccharides
-
 ?
chitosan + H2O substrate colloidal chitosan, 85% deacetylated Lysobacter sp. IB-9374 chitosan oligosaccharides
-
 ?
colloidal chitin + H2O 5.1% of the activity with colloidal chitosan Lysobacter sp. ?
-
 ?
colloidal chitin + H2O 5.1% of the activity with colloidal chitosan Lysobacter sp. IB-9374 ?
-
 ?
glycol chitosan + H2O substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan Lysobacter sp. glycol chitosan oligosaccharides
-
 ?
glycol chitosan + H2O substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan Lysobacter sp. IB-9374 glycol chitosan oligosaccharides
-
 ?
additional information at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan Lysobacter sp. ?
-
 ?
additional information at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan Lysobacter sp. IB-9374 ?
-
 ?
water-soluble chitosan + H2O substrate 40-50% deacetylated, 7.6% of the activity with colloidal chitosan Lysobacter sp. chitosan oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.

Synonyms

Synonyms Comment Organism
Cel8A
-
 Lysobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, 20 mM acetate pH 5.0, stable up to 40°C Lysobacter sp.
50
-
 30 min, 20 mM acetate pH 5.0, 95% loss of activity Lysobacter sp.
70
-
 optimum for chitosanase acivity Lysobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 and 7.0, at pH 7.0 higher chitosanase activity than at pH 5.0 Lysobacter sp.
7
-
 and 5.0, at pH 7.0 higher chitosanase activity than at pH 5.0 Lysobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 more than 80% of maximum activity Lysobacter sp.