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Literature summary for 3.2.1.132 extracted from
- Biochemical characteristics of chitosanase from the Indonesian Bacillus licheniformis MB-2 (2006), Mol. Biotechnol., 33, 93-102.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidinium hydrochloride | 2 M, 32% residual activity | Bacillus licheniformis |  |
| additional information | enzyme is not affected by presence of 1 M NaCl or 6 M urea | Bacillus licheniformis | |
| Ni2+ | 38% residual activity | Bacillus licheniformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus licheniformis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 35% increase in activity | Bacillus licheniformis | |
| Mn2+ | 45% increase in activity | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, SDS-PAGE | Bacillus licheniformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus licheniformis | chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus | ? | - |
? | |
| additional information | Bacillus licheniformis MB-2 | chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 10%, 57% residual activity | Bacillus licheniformis |
| propanol | 10%, 57% residual activity | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis MB-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | 85% deacetylated soluble chitosan is the most susceptible substrate | Bacillus licheniformis | chitopentaose + chitohexaose | products after 24 h incunation at 70°C | ? | |
| chitosan + H2O | 85% deacetylated soluble chitosan is the most susceptible substrate | Bacillus licheniformis MB-2 | chitopentaose + chitohexaose | products after 24 h incunation at 70°C | ? | |
| glycol chitosan + H2O | - |
Bacillus licheniformis | glycol chitosan oligosaccharides | - |
? | |
| glycol chitosan + H2O | - |
Bacillus licheniformis MB-2 | glycol chitosan oligosaccharides | - |
? | |
| additional information | chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus | Bacillus licheniformis | ? | - |
? | |
| additional information | no substrates: colloidal and glycol chitin | Bacillus licheniformis | ? | - |
? | |
| additional information | chitosan oligosaccharides, the enzymatic degradation products, show an inhibitory effect on growth of Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monomcytogenens, Bacillus cereus, Escherichia coli, Staphylococcus aureus | Bacillus licheniformis MB-2 | ? | - |
? | |
| additional information | no substrates: colloidal and glycol chitin | Bacillus licheniformis MB-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life 26.6 min | Bacillus licheniformis |
| 80 | - |
half-life 18.4 min | Bacillus licheniformis |
| 90 | - |
half-life 16.7 min, but enzyme retains activity for up to 8 h in presence of 1 mM MnCl2 | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Bacillus licheniformis |
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