Cloned (Comment) | Organism |
---|---|
expressed in Streptomyces lividans strain TK24 and Escherichia coli Gold cells | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
D40G | the mutant shows drastic loss of activity | Streptomyces sp. |
D40G/R42E | the mutant shows drastic loss of activity | Streptomyces sp. |
D40G/R42K | the mutant shows drastic loss of activity | Streptomyces sp. |
R42E | the mutant shows drastic loss of activity | Streptomyces sp. |
R42K | the mutant shows drastic loss of activity | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chitosan | the wild type enzyme shows a Ki value of 1.547 mg/ml | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Streptomyces sp. | the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | P33665 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
mutant enzyme D40G/R42E, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
0.018 | - |
mutant enzyme D40G/R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
0.9 | - |
mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
1.4 | - |
mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
1.6 | - |
mutant enzyme D40G, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
52.9 | - |
wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) | Streptomyces sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Csn | - |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
chitosan | mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. | |
1.6 | - |
chitosan | mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. | |
10.7 | - |
chitosan | wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptomyces sp. |