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Literature summary for 3.2.1.132 extracted from

  • Wang, S.L.; Chen, S.J.; Wang, C.L.
    Purification and characterization of chitinases and chitosanases from a new species strain Pseudomonas sp. TKU015 using shrimp shells as a substrate (2008), Carbohydr. Res., 343, 1171-1179.
    View publication on PubMed
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 2% nonionic surfactant of Triton X-100 activates by 51% at 25°C for 30 min Pseudomonas sp.
Tween 20 2% nonionic surfactant of Tween 20 activates by 38% at 25°C for 30 min Pseudomonas sp.
Tween 40 2% nonionic surfactant of Tween 40 activates by 6% at 25°C for 30 min Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
EDTA 10 mM inhibits by 86% at 25°C for 30 min Pseudomonas sp.
Fe2+ 5 mM inhibits by 67% at 25°C for 30 min Pseudomonas sp.
Mn2+ 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
PMSF 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
SDS 2 mM of the anionic surfactant SDS completely inhibits at 25°C for 30 min Pseudomonas sp.
Triton X-100 0.5% nonionic surfactant of Triton X-100 inhibits by 56% at 25°C for 30 min Pseudomonas sp.
Tween 20 0.5% nonionic surfactant of Tween 20 inhibits by 16% at 25°C for 30 min Pseudomonas sp.
Tween 40 0.5% nonionic surfactant of Tween 40 inhibits by 29% at 25°C for 30 min Pseudomonas sp.
Zn2+ 5 mM inhibits by 21% at 25°C for 30 min Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM activates by 11% at 25°C for 30 min Pseudomonas sp.
additional information 5 mM of Mg2+, Ba2+ have no effect on activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS–PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. TKU015
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, to homogeneity, 6.4fold Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.3
-
 culture supernatant Pseudomonas sp.
59.1
-
 6.4fold purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan Pseudomonas sp. ?
-
 ?
chitosan + H2O activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan Pseudomonas sp. TKU015 ?
-
 ?
additional information no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan Pseudomonas sp. ?
-
 ?
additional information no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan Pseudomonas sp. TKU015 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS–PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
Chs1
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 maintains its initial activity, has almost half of its activity at 50°C but is completely inactivated at 70°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9
-
 Pseudomonas sp.