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Literature summary for 3.2.1.132 extracted from

  • Takasuka, T.E.; Bianchetti, C.M.; Tobimatsu, Y.; Bergeman, L.F.; Ralph, J.; Fox, B.G.
    Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA-E (2014), Proteins, 82, 1245-1257.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 10% (w/v) MEPEG 5K, 300 mM tetramethylammonium chloride, and 100 mM Bis-Tris, pH 6.5, at 4°C Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Streptomyces sp.
-
 ?
-
 ?
chitosan + H2O Streptomyces sp. SirexAA-E
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. G2NRC4
-
-
Streptomyces sp. SirexAA-E G2NRC4
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, and gel filtration Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Streptomyces sp. ?
-
 ?
chitosan + H2O
-
 Streptomyces sp. SirexAA-E ?
-
 ?
additional information the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed Streptomyces sp. ?
-
 ?
additional information the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed Streptomyces sp. SirexAA-E ?
-
 ?

Synonyms

Synonyms Comment Organism
SACTE_5457
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 chitosan chitosan with 75-85% deacetylation, at pH 6.0 and 45°C Streptomyces sp.
10
-
 chitosan chitosan with less than 75% deacetylation, at pH 6.0 and 45°C Streptomyces sp.
11.2
-
 chitosan chitosan with more than 90% deacetylation, at pH 6.0 and 45°C Streptomyces sp.