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3.2.1.132: chitosanase

This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.

Word Map on EC 3.2.1.132

Reaction

2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose
+
H2O
=
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose
 +
2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.132 chitosanase

Specific Activity

Specific Activity on EC 3.2.1.132 - chitosanase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
mutant enzyme D40G/R42E, at 37°C in 50 mM sodium acetate buffer (pH 5.5)
0.002
0.005316
-
culture supernatant
0.006
-
culture supernatant, at pH 7.0 and 60°C
0.0065
-
crude extract, at pH and °C
0.011
mutant T45E
0.018
mutant enzyme D40G/R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5)
0.02
mutant D40G/T45E
0.032
-
enzyme HEWL
0.055
-
enzyme BCVC-4
0.07
mutant E36A/D40G
0.075
-
enzyme BCVC-3
0.087
-
enzyme BCVC-5
0.114
-
enzyme BCVC-6
0.177
-
after 88.5fold purification, at pH 4.0 and 40°C
0.18
-
after 30fold purification, at pH 7.0 and 60°C
0.243
-
isoform B, after 115.1fold purification, at pH 4.5 and 50°C
0.3
mutant E36Q/D40G
0.402
-
pH 5.2, 60°C
0.567
-
enzyme Yam
0.753
-
isoform A, after 115.1fold purification, at pH 4.5 and 50°C
0.9
mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5)
1.291
-
enzyme BCLVC
1.38 - 1.53
-
activity of enzyme immobilized on liposomes
1.4
mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5)
102.3
126
-
after TSK-gel HW-55F gel filtration
154.8
163.8
-
after Mono-S cation exchange chromatography with 0.06 to 0.08 M KCl
17.1
-
culture broth, pH 6.0, 50°C
17.2
-
enzyme I after after Sephadex G-100 chromatography
196
-
after purification on Super Q Toyopearl
2.62
-
after Toyopearl HW-50 chromatography
2.8
-
527fold purified enzyme
250.87
-
pH 5.6, 55°C, mutant enzyme K163A
261.73
-
pH 5.6, 55°C, mutant enzyme E160A
2819
-
after DEAE-Sephacel ion exchange chromatography
283.93
-
pH 5.6, 55°C, mutant enzyme E160Q
3.411
-
enzyme BCVC-1
3.9
65fold purified enzyme
328.05
-
pH 5.6, 55°C, mutant enzyme T114A
335.07
-
pH 5.6, 55°C, wild-type enzyme
35.61
412
-
after 24.1fold purification, pH 6.0, 50°C
444
-
after Superdex 75 HR gel filtration
489.8
-
isoenzyme PSC-II
58.9
-
Bio-Gel A eluate of the recombinant protein
59.1
-
6.4fold purified enzyme
6.03
-
pH 7, 37°C
7
-
50fold purified enzyme
7.59
-
culture filtrate, at pH 5.7 and 37°C
733.5
-
isoenzyme PSC-III
742.6
-
isoenzyme PSC-I
8.8
-
after 1.16fold purification, at pH 5.7 and 37°C
82
-
pH 4.8, 50°C
850
-
purified enzyme
89.6
-
desalted extraction
9.3
-
culture supernatant
additional information