3.2.1.132: chitosanase

This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.

Reaction

Synonyms

actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.132 chitosanase

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Results	in table
17	Activating Compound
2397	AA Sequence
56	Application
1	CAS Registry Number
51	Cloned(Commentary)
11	Crystallization (Commentary)
8	Expression
6	General Information
7	General Stability
1	IC50 Value
318	Inhibitors
8	kcat/KM [mM/s]
55	KM Value [mM]
36	Localization
71	Metals/Ions
182	Molecular Weight [Da]
174	Natural Substrates/ Products (Substrates)
16	Organic Solvent Stability
504	Organism
2	Pathway
24	PDB ID
135	pH Optimum
28	pH Range
37	pH Stability
37	pI Value
10	Posttranslational Modification
174	Protein Variants
97	Purification (Commentary)
1	Reaction
1	Reaction Type
299	Reference
38	Source Tissue
106	Specific Activity [micromol/min/mg]
5	Storage Stability
669	Substrates and Products (Substrate)
182	Subunits
119	Synonyms
1	Systematic Name
131	Temperature Optimum [°C]
19	Temperature Range [°C]
126	Temperature Stability [°C]
42	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.2.1.132 - chitosanase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

Aeromonas sp.

-

activates 28% at 1 mM

707019

Chelating agents

Bacillus sp. (in: Bacteria)

-

especially EDTA, activate

646743, 646792

Co2+

Trichoderma viride

-

2.5 mM, 16% increase in activity

678006

diethyl dicarbonate

Bambusa oldhamii

-

isoform A shows 111% activity at 2.5 mM

731964

Mn2+

Trichoderma viride

-

3 mM, 18% increase in activity

678006

p-hydroxymercuribenzoate

Bambusa oldhamii

-

isoform A shows 112% activity at 0.5 mM

731964

Triton X-100

2 entries

Triton X-114

uncultured bacterium

A0A0A7RCI7

1.0% v/v, 1.38fold increase in activity

751337

Tween 20

Pseudomonas sp.

-

2% nonionic surfactant of Tween 20 activates by 38% at 25°C for 30 min

691643

Tween 40

2 entries

Tween 80

2 entries

additional information

2 entries

-

Triton X-100

Pseudomonas sp.

-

2% nonionic surfactant of Triton X-100 activates by 51% at 25°C for 30 min

691643

Triton X-100

uncultured bacterium

A0A0A7RCI7

1.0% v/v, 1.35fold increase in activity

751337

Tween 40

Pseudomonas sp.

-

2% nonionic surfactant of Tween 40 activates by 6% at 25°C for 30 min

691643

Tween 40

uncultured bacterium

A0A0A7RCI7

1.0% v/v, 1.85fold increase in activity

751337

Tween 80

Bacillus thuringiensis

-

0.25-1%, increases activity 7-40%

665017

Tween 80

uncultured bacterium

A0A0A7RCI7

1.0% v/v, 1.29fold increase in activity

751337

additional information

Serratia marcescens

-

chitosanase is activated after keeping at 4°C for 10 days, in the presence of 25% (v/v) toluene, acetonitrile, and butanol

697120

-

additional information

Streptomyces griseus

-

direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes with membrane of Streptomyces griseus cell under heat stress at 41°C increases chitosanase production and release to 2.2times higher than that at 37°C without the 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes. Lipid mimicking cell membrane liposome under heat at 41°C in the presence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes is 17% of initially-entrapped chitosanase while it is only 1% in the absence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes, even under a heat stress at 41°C

699049

-