Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.132 extracted from

  • Aktuganov, G.E.; Shirokov, A.V.; Melent'ev, A.I.
    Isolation and characterization of chitosanase from the strain Bacillus sp. 739 (2003), Appl. Biochem. Microbiol., 39, 469-474.
No PubMed abstract available
Search for more literature for 3.2.1.132

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 25 mg/ml with colloidal chitosane as substrate Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) K-1
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) 739
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 K-1
-
Bacillus sp. (in: Bacteria)
-
 739
-
Bacillus sp. (in: Bacteria) 739
-
 739
-
Bacillus sp. (in: Bacteria) K-1
-
 K-1
-

Purification (Commentary)

Purification (Comment) Organism
739 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.62
-
 after Toyopearl HW-50 chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) 739 ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 additional information
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) 739 additional information
-
 ?

Subunits

Subunits Comment Organism
? x * 46000 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-maximum inactivation after 1 h Bacillus sp. (in: Bacteria)
60
-
 loss of 75% activity after 15 min Bacillus sp. (in: Bacteria)
70
-
 loss of 95% activity after 15 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Bacillus sp. (in: Bacteria)