3.2.1.132: chitosanase
This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.
Word Map on EC 3.2.1.132
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3.2.1.132
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chitosans
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chitooligosaccharides
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deacetylation
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oligosaccharide
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glycoside
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colloidal
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chitinases
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glcn
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endo-type
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biocontrol
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paenibacillus
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circulans
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chitotetraose
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chitosanolytic
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d-glucosamine
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food industry
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chitinolytic
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chitotriose
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synthesis
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biotechnology
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glucanase
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analysis
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industry
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pharmacology
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coss
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medicine
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chitopentaose
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chitooligomers
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lichenan
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microbacterium
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agriculture
- 3.2.1.132
- chitosans
- chitooligosaccharides
-
deacetylation
- oligosaccharide
- glycoside
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colloidal
- chitinases
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glcn
-
endo-type
-
biocontrol
- paenibacillus
- circulans
- chitotetraose
-
chitosanolytic
- d-glucosamine
- food industry
-
chitinolytic
- chitotriose
- synthesis
- biotechnology
- glucanase
- analysis
- industry
- pharmacology
- coss
- medicine
- chitopentaose
-
chitooligomers
- lichenan
- microbacterium
- agriculture
Reaction
Synonyms
actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase
ECTree
3.2.1.132 chitosanaseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 3.2.1.132 - chitosanase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2,4-Dinitro-1-fluorobenzene
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isoform A shows 95% residual activity at 0.625 mM, isoform B shows 84% residual activity at 0.625 mM
2-Hydroxy-5-nitrobenzyl bromide
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69% inhibition at 5 mM, 33% inhibition at 1 mM
Mo2+
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94% inhibition of chitosanase I and 88% inhibition of chitosanase II
N-Acetylimidazole
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isoform A shows 96% residual activity at 5 mM, isoform B shows 84% residual activity at 5 mM
phenylmethylsulfonyl fluoride
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isoform B shows 86% residual activity at 2.5 mM
sulfhydryl reagents
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glutathione or L-cysteine restores activity
Woodward's reagent K
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N-ethyl-5-phenylisoazoline-3'-sulfonate, isoform A shows 95% residual activity at 50 mM, isoform B shows 1% residual activity at 50 mM
Ag+
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isoform A shows 91% residual activity at 1 mM, isoform B shows 86% residual activity at 1 mM
Al3+
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1 mM, 86.5% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 87% inhibition of wild-type enzyme
Ba2+
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5 mM inhibits by 15%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Ca2+
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5 mM inhibits by 10%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Cu2+
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59% inhibition of chitosanase I and 48% inhibition of chitosanase II
Cu2+
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5 mM, about 40% loss of activity of chitosanase A, about 30% loss of activity of chitosanase B
Cu2+
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5 mM inhibits by 41%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Cu2+
1 mM completely inhibits, with colloidal chitosan as substrate
Cu2+
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1 mM, complete inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) and of wild-type enzyme
EDTA
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5 mM inhibits by 10%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Fe2+
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5 mM, about 30% loss of activity of chitosanase A, about 25% loss of activity of chitosanase B
Fe2+
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5 mM inhibits by 77%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Fe3+
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1 mM, 11% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 13% inhibition of wild-type enzyme
Hg2+
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81% inhibition of chitosanase I and 80% inhibition of chitosanase II
Hg2+
1 mM completely inhibits, with colloidal chitosan as substrate
Mg2+
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5 mM inhibits by 14%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
Mn2+
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5 mM inhibits by 17%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
N-bromosuccinimide
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isoform A shows 12% residual activity at 2.5 mM, isoform B is completely inhibited at 2.5 mM
Ni2+
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1 mM, 22% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM,10% inhibition of wild-type enzyme
SDS
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2 mM of the anionic surfactant SDS completely inhibits at 25°C for 30 min
Triton X-100
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0.5% nonionic surfactant of Triton X-100 inhibits by 56% at 25°C for 30 min
Tween 20
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0.5% nonionic surfactant of Tween 20 inhibits by 16% at 25°C for 30 min
Tween 40
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0.5% nonionic surfactant of Tween 40 inhibits by 29% at 25°C for 30 min
Zn2+
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5 mM, about 30% loss of activity of chitosanase A, about 40% loss of activity of chitosanase B
Zn2+
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5 mM inhibits by 18%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C
additional information
antifungal effect of CtoA against Rhizopus oryzae IAM6252 is drastically enhanced by the simultaneous addition of the family 19 chitinase ChiC from Streptomyces griseus
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additional information
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not inhibited by phenylmethylsulfonyl fluoride, TKUPSP018, TKUPSP073, TKUPSP011, TKUPSP035, TKUPSP080, and TKUPSP062
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additional information
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enzyme is not affected by presence of 1 M NaCl or 6 M urea
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additional information
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in the presence of 2% Tween 20, Tween 40, Triton X-100 (non-ionic surfactant), and 2 mM SDS (anionic surfactant), the enzyme retains almost original activity
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additional information
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the enzyme is very stable under incubation with EDTA, EGTA, o-phenanthroline, N-ethylmaleimide, monoiodoacetate and EDAC (5 mM each), beta-mercaptoethanol and dithiothreitol (2 mM each), N-bromosuccinimide (1 mM) and p-chloromercuribenzoate (0.1 mM)
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additional information
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not inhibited by diethyl dicarbonate, 1,2-cyclohexanedione, and p-hydroxymercuribenzoate
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additional information
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ethanol, isopropanol and dimethyl sulfoxide (5 mM -100 mM) have no effect in activity
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additional information
1 mM Mg2+, Ca2+, Fe3+, Zn2+, Ba2+ and Pb2+ have little or no effect on activity
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additional information
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5 mM Ba2+, Mg2+ and Ca2+ have little effect on activity at 37°C for 30 min. After keeping at 25°C for 10 days, the chitosanase is inactivated in the presence of 25% (v/v) ethanol and ethyl ether
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additional information
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not influenced by 5 mM phenylmethylsulfonyl fluoride, 0.5 mM SDS, 0.5-2% (v/v) Tween 20, 0.5-2% (v/v) Tween 40, and 0.5-2% (v/v) Triton X-100
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additional information
extent of growth inhibition is dependent on chitosan concentration, average molecular mass, the pH of the medium and salt composition. Growth inhibition can be suppressed by the expression of CsnN174 in Escherichia coli JM109
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