3.2.1.132: chitosanase

This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.

Reaction

Synonyms

actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.132 chitosanase

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Results	in table
17	Activating Compound
2397	AA Sequence
56	Application
1	CAS Registry Number
51	Cloned(Commentary)
11	Crystallization (Commentary)
8	Expression
6	General Information
7	General Stability
1	IC50 Value
318	Inhibitors
8	kcat/KM [mM/s]
55	KM Value [mM]
36	Localization
71	Metals/Ions
182	Molecular Weight [Da]
174	Natural Substrates/ Products (Substrates)
16	Organic Solvent Stability
504	Organism
2	Pathway
24	PDB ID
135	pH Optimum
28	pH Range
37	pH Stability
37	pI Value
10	Posttranslational Modification
174	Protein Variants
97	Purification (Commentary)
1	Reaction
1	Reaction Type
299	Reference
38	Source Tissue
106	Specific Activity [micromol/min/mg]
5	Storage Stability
669	Substrates and Products (Substrate)
182	Subunits
119	Synonyms
1	Systematic Name
131	Temperature Optimum [°C]
19	Temperature Range [°C]
126	Temperature Stability [°C]
42	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.2.1.132 - chitosanase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystal structure determined at 1.5 A resolution in the active form and at 2.0 A resolution in the inactive form. Hanging-drop vapor-diffusion method

Bacillus sp. (in: Bacteria)

-

666032

two different crystal forms, ChoK-a and ChoK-i are obtained at pH 6.4 and pH 3.7, respectively. ChoK-a crystals belong to space group P2(1)2(1)2(1). From the unit-cell parameters, a = 70.8 A, b = 98.1 A, c = 115.8 A. The number of protein molecules in the asymmetric subunit is estimated to be two. The crystal diffracts well to 1.5 A resolution. The ChoK-i crystal belongs to space group I222, with unit-cell parameters a = 85.1 A, b = 91.1 A, c = 131.5 A. There is one molecule in the asymmetric subunit and the resolution is restricted to 2.0 A

Bacillus sp. (in: Bacteria)

-

663489

in complex with hexaglucosamine, hanging drop vapor diffusion method, using 0.05 M KH2PO4 and 23% (w/v) PEG 8000 using 0.7 M NaCl as the reservoir at 15°C

Microbacterium sp.

A7KBW5

731275

crystal structure of mutant enzyme K218P

Niallia circulans

P33673

665430

sitting drop vapor diffusion method at 20°C. The crystal structure of mutant K218P reveals that the main chain and side chain structures of the loop comprising Lys218 are effected by the mutation. It is concluded that the flexible loop comprising Lys128 plays an important role in substrate binding

Niallia circulans

-

665331

sitting drop vapour diffusion method at 20°C, orthorhombic space group P21212, two globular upper and lower domains which generate the active site cleft for the substrate binding

Niallia circulans

P33673

646770

hanging drop vapor diffusion at 15°C

Pseudomonas sp. A-01

Q8KZM5

749948

-

Streptomyces sp.

-

646787

hanging drop crystallization with 30 mg/ml protein in 20 mM acetate, pH 5.5 mixed with 0.1 volume of a 0.5 M potassium phosphate, 20% w/v PEG 8000 solution, monoclinic space group P21

Streptomyces sp.

-

646761

sitting drop vapor diffusion method, using 10% (w/v) MEPEG 5K, 300 mM tetramethylammonium chloride, and 100 mM Bis-Tris, pH 6.5, at 4°C

Streptomyces sp.

G2NRC4

732891

the substrate binding cleft is composed of six monosaccharide binding subsites

Streptomyces sp.

-

646779