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Literature summary for 3.2.1.132 extracted from

  • Chen, X.; Zhai, C.; Kang, L.; Li, C.; Yan, H.; Zhou, Y.; Yu, X.; Ma, L.
    High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris (2012), Biotechnol. Lett., 34, 689-694.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Aspergillus fumigatus

General Stability

General Stability Organism
the enzyme retaines 70% activity after incubation with 10 M urea at room temperature for 30 min Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 10 mM inhibits more than 50% of the enzyme activity Aspergillus fumigatus
Cu2+ 10 mM inhibits more than 50% of the enzyme activity Aspergillus fumigatus
Fe3+ strongly inhibited by 10 mM Aspergillus fumigatus
SDS strongly inhibited by 0.1% (w/v) SDS Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Aspergillus fumigatus
-
 chitosan oligosaccharides
-
 ?
chitosan + H2O Aspergillus fumigatus Y2K
-
 chitosan oligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q875I9 synonym Aspergillus fumigatus
-
Aspergillus fumigatus Y2K Q875I9 synonym Aspergillus fumigatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Aspergillus fumigatus chitosan oligosaccharides
-
 ?
chitosan + H2O
-
 Aspergillus fumigatus Y2K chitosan oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
Csn
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 65
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 about 50% activity at 45°C, about 80% activity at 50°C, 100% activity from 55-65°C, about 80% activity at 70°C, about 50% activity at 75°C Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 100 the enzyme has half-lives of 2.5 h at 80°C, 1 h at 90°C and 32 min at 100°C. Almost full enzyme activity remains after 1 h at 80°C, 30 min at 90°C and 10 min at 100°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 more than 50% activity between pH 4.0 and 7.5, more than 90% activity between pH 4.5 and 7.0 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 the enzyme retains 100% activity after 1 h in the pH range 3.5-8.0 Aspergillus fumigatus