Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | slightly inactivated at 0.5 mM | Bacillus cereus | |
Mg2+ | slightly inactivated at 0.5 mM | Bacillus cereus | |
NaCl | slightly inactivated at 0.5 mM | Bacillus cereus | |
Zn2+ | slightly inactivated at 0.5 mM | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000327 | - |
chitosan | at pH 8.5 and 37°C | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | chitosan degrading activity is enhanced by 15% in the presence of calcium ions (8 mM) | Bacillus cereus | |
Mg2+ | slightly activated by Mn2+ | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Bacillus cereus | - |
chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
chitosan + H2O | Bacillus cereus GU-02 | - |
chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus GU-02 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Superdex 75 gel filtration | Bacillus cereus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate + H2O | 12.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
alginate + H2O | 12.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
beta-glucan + H2O | 32.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
beta-glucan + H2O | 32.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
chitosan + H2O | - |
Bacillus cereus | chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
chitosan + H2O | - |
Bacillus cereus GU-02 | chitosan oligosaccharides | chitosan oligosaccharides comprise oligomers with degree of polymerization mainly from dimers to pentamers | ? | |
fucoidan + H2O | 16.9% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
fucoidan + H2O | 16.9% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
hyaluronic acid + H2O | 26.7% activity compared to chitosan | Bacillus cereus | ? | - |
? | |
hyaluronic acid + H2O | 26.7% activity compared to chitosan | Bacillus cereus GU-02 | ? | - |
? | |
sulfate chitin + H2O | 9.6% activity compared to chitosan | Bacillus cereus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
actase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 50 | about 55% activity at 27°C, about 62% activity at 32°C, 100% activity at 37°C, about 90% activity at 42°C, and about 80% activity at 50°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | about 55% activity at pH 3.0, about 57% activity at pH 5.0, about 80% activity at pH 6.0, 100% activity at pH 9.0, about 75% activity at pH 10.0-11.0 | Bacillus cereus |