Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Bacillus subtilis | |
N-bromosuccinimide | - |
Bacillus subtilis | |
p-hydroxymercuribenzoic acid | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Bacillus subtilis |
41000 | - |
1 * 41000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Bacillus subtilis | - |
? | - |
? | |
chitosan + H2O | Bacillus subtilis IMR-NK1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
IMR-NK1 | - |
Bacillus subtilis IMR-NK1 | - |
IMR-NK1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2819 | - |
after DEAE-Sephacel ion exchange chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | - |
Bacillus subtilis | ? | - |
? | |
chitosan + H2O | - |
Bacillus subtilis IMR-NK1 | ? | - |
? | |
chitosan + H2O | degrades 60-94% deacetylated chitosan most effectively | Bacillus subtilis | additional information | end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length | ? | |
chitosan + H2O | degrades 60-94% deacetylated chitosan most effectively | Bacillus subtilis IMR-NK1 | additional information | end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length | ? | |
colloidal chitin + H2O | - |
Bacillus subtilis | ? | - |
? | |
colloidal chitin + H2O | - |
Bacillus subtilis IMR-NK1 | ? | - |
? | |
glycol chitosan + H2O | - |
Bacillus subtilis | ? | - |
? | |
glycol chitosan + H2O | - |
Bacillus subtilis IMR-NK1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
rather stable for 20 min | Bacillus subtilis |
40 | - |
rather stable for 20 min | Bacillus subtilis |
45 | - |
gradual inactivation of the enzyme | Bacillus subtilis |
50 | - |
gradual inactivation of the enzyme | Bacillus subtilis |
55 | - |
significant loss in activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable at 25°C for 1 h | Bacillus subtilis |