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3.2.1.132: chitosanase

This is an abbreviated version!
For detailed information about chitosanase, go to the full flat file.

Word Map on EC 3.2.1.132

Reaction

2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose
+
H2O
=
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose
 +
2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

actase, AsChi, BspCsnMN, Cel7A, Cel8A, ChA, CHI, ChiN, Chit A, Chit B, chitosan N-acetylglucosaminohydrolase, chitosanase, chitosanase 1, chitosanase CsnA, chitosanase EAG1, chitosanase II, chitosanase OU01, ChiX, CHO, Cho-GG, ChoK, Chs1, Csn, CSN-174, CSN-7M, Csn-PD, Csn1, Csn2, CsnA, CsnB, CsnM, CSNMHKI, CsnN174, CsnTS, csnw2, Cto1, CtoA, Culf-Z, endo-acting chitinase, endo-chitosanase, endochitosanase, family 46 chitosanase, GH-75 chitosanase, GsCsn46A, ImmB-Z, MH-K1 chitosanase, More, mschito, N174 chitosanase, PgChP, SACTE_5457, SCO0677, StrCSN1, StrCSN2, TKU032 chitosanase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.132 chitosanase

Cloned

Cloned on EC 3.2.1.132 - chitosanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified DNA fragment ligated into a pGEM T-easy vector, subsequently subcloned from the T-vector into a pET vector, expressed as 6 x His tag fused at its N-terminal in the Escherichia coli strain BL21(DE3) as a soluble and active form
-
chimeric chitosanase and wild-type Csn overexpressed from vector pET22b(+) in Escherichia coli strain BL21 (DE3)
csn gene under control of the Aspergillus nidulans gpdA promoter and Aspergillus nidulans trpC terminator, introduced back into the Fusarium solani genome by Agrobacterium tumefaciens-mediated transformation, and usage of the herbicide-resistance gene bar from Streptomyces hygroscopicus as selection marker. Construction of a vector based on pCAMBIA 1300, overexpression in Fusarium solani 0114
expressed in Aspergillus oryzae
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3)
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Pichia pastoris GS115 cells
expressed in Saccharomyces cerevisiae strain BY4741/sed1DELTA
expressed in Streptomyces lividans strain TK24 and Escherichia coli Gold cells
expression as a fusion protein in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3)
-
expression in Saccharomyces cerevisiae
-
expression in Streptomyces lividans strain TK24
-
expression in Streptomyces lividans TK24
expression in yeast cells as a whole-cell biocatalyst
-
expression of CsnN174 in Escherichia coli JM109. T45S chitosanase mutant introduced into Streptomyces lividans
expression of GST-tagged shuffled mutant enzymes Y18 and Y20 in Escherichia coli strain BL21(DE3)
-
gene cho, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
genomic sequence of Csn2 cloned from the DNA library by PCR
heterologously expressed in Escherichia coli BL21 (DE3)
overexpression of Csn1 in Fusarium solani strain T2 using transformation by Agrobacterium tumefaciens strain LBA 4404 and expression vector pCIR
-
overexpression of wild-type enzyme and mutant enzymes E122Q, D183N and E309Q in Escherichia coli
-
subcloning and library construction in Escherichia coli strain DH5alpha, expression of the enzyme in Saccharomyces cerevisiae cells displayed at the cell surface, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae
-
the enzyme is expressed recombinantly as a periplasmic enzyme in Escherichia coli strain BMS172
-
the mature CsnW2 protein is fused to His-tag at C-terminus and expressed in Pichia pastoris X-33
wild-type and mutant overexpressed from vector pET15b/CSO2 in Escherichia coli BL21(DE3)
wild-type and mutants overexpressed in Brevibacillus choshinensis HDP31-M3 carrying the cto1 gene on expression plasmid vector pNCMO2
wild-type and mutants subcloned into vector pFD666 and expressed in Streptomyces lividans TK-24
-