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0 - 90
-
the enzyme is stable over a temperature range of 0-50°C and shows about 20% of the initial activity after heating at 90°C for 10 min
10
-
most stable at 10°C, stability of chitosanase I droppes at higher temperatures and most activity is lost above 60°C
20 - 70
-
all three isoforms are stable in this range for 30 min
27 - 50
-
the enzyme is stable between 27 and 50°C for up to 3 h
30 - 40
-
stable at this range
35.55
-
Tm-value of mutant enzyme E160A
36.02
-
Tm-value of mutant enzyme E160Q
37.36
-
Tm-value of mutant enzyme T114A
38.86
-
Tm-value of mutant enzyme K163A
40 - 50
-
its half-life is only several min at 60°C. At lower temperatures, the enzyme is quite stable, its half-life is extended to several hours at 50 and 40°C. No detectable activity loss at room temperature for several days
40 - 60
-
the purified chitosanase is continuously thermostable at 40°C and the stability is gradually decreased when the temperature is increased to 50°C but rapidly decreases at 60°C
40.36
-
Tm-value of wild-type enzyme
42.5
melting temperature of the wild type enzyme
43.4
-
transition temperature in absence of chitosan
45 - 55
-
the enzyme is gradually inactivated during incubation at temperature above 45°C and is completely inactivated at 55°C after 15 min, at 55°C this instability is abolished in the presence of reaction products chitotriose and chitotetraose (2.5 mM each). The residual activities of the enzyme after 1 h of incubation at 60°C with 10 mM chitotriose or chitotetraose are 35.5% and 59.5%, respectively
48.9
-
T50-value, wild-type enzyme
5 - 30
-
in absence of substrate the chitosanase is stable between 5°C and 30°C
50 - 55
half-lives of Csn2 at 50°C and 55°C are 30 min and 11 min, respectively
54
-
wild-type, transition temperature in absence of chitosan
57.5
Tm-value of mutant enzymes D318R
57.7
Tm-value of mutant enzymes D318K
57.8
-
T50-value, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5)
60 - 80
-
the chitosanase maintains its initial activity at less than 60°C, the chitosanase is inactivated completely at 80°C
61
-
both wild-type and mutants A104L, K164R are active over 90 min
63.6
Tm-value of mutant enzyme D318A
67.1
Tm-value of wild-type enzyme
67.5
Tm-value of mutant enzyme D318N
68.6
-
transition temperature in presence of chitosan at 6 g/l
69.5
-
wild-type, transition temperature in presence of chitosan at 6 g/l
70 - 85
the half-life of the enzyme at 70°C in the presence of chitosan substrate is more than 20 h. Maximal activity is observed at 80°C for 10 min and at 85°C for 5 and 3 min incubation times
80 - 100
the enzyme has half-lives of 2.5 h at 80°C, 1 h at 90°C and 32 min at 100°C. Almost full enzyme activity remains after 1 h at 80°C, 30 min at 90°C and 10 min at 100°C
81.1
-
consensus mutant, transition temperature in presence of chitosan at 6 g/l
83.3
-
mutant A104L, transition temperature in presence of chitosan at 6 g/l
85.1
-
mutant K164R, transition temperature in presence of chitosan at 6 g/l
90
-
half-life 16.7 min, but enzyme retains activity for up to 8 h in presence of 1 mM MnCl2
0
-
stable
100
-
10 min, 50% loss of activity
100
-
the enzyme recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively
20
-
purified enzyme, pH 5.8, 2 h, 98% activity remaining
25 - 40
-
maintains its initial activity from 25 to 40°C but is completely inactivated at 80°C
25 - 40
-
maintains its initial activity, has almost half of its activity at 50°C but is completely inactivated at 70°C
25 - 50
-
the enzyme is stable between 25 and 50°C, maintains its initial activity from 25 to 40°C and has 50% of its activity at 50°C, and is inactivated at 80°C
25 - 50
-
maintains more than 90% of its initial activity between 25 and 50°C, but is rapidly inactivated retaining only 60% of its initial activity at 60°C, and is completely inactivated at greater than 70°C
30
-
purified enzyme, pH 5.8, 2 h, 92% activity remaining
30
-
pH 5.0, 30 min, stable
30
24 h, enzyme retains 96.5% of its initial activity
30
-
rather stable for 20 min
30
-
1 h, enzyme retains 25.4% of its initial activity
30 - 60
-
stable at, chitosanase A and chitosanase B
30 - 60
-
more than 90% of the initial activities are retained after incubation at 30, 40, 50, and 60°C for 30 min
30 - 80
-
after 15 min incubation the enzyme shows 100% activity at 30°C, about 90% at 40°C, about 85% at 50°C, about 82% at 60°C, about 80% at 70°C, about 60% at 80°C, about 40° at 90°C, and about 20% activity at 100°C
30 - 80
-
isoform A is stable between 30 and 80°C, whereas isoform B is stable between 30 and 50°C after 60 min incubation
37
-
pH 6.0-8.0, stable, unstable at elevated temperatures
37
-
denaturation in 1-2 h, stabilized by 1.0 mM Ca2+
40
-
purified enzyme, pH 5.8, 2 h, 85% activity remaining
40
-
15 min, stable up to
40
-
pH 5.0, 30 min, stable
40
-
pH 6, 1 h, stable below
40
24 h, enzyme retains 77.9% of its initial activity
40
-
rather stable for 20 min
40
-
30 min, 20 mM acetate pH 5.0, stable up to 40°C
40
-
1 h, enzyme retains 15.8% of its initial activity
40
-
rapid decrease of activity above
45
-
stable for 1 h
45
-
pH 6.8, 15 min, stable below
45
24 h, enzyme retains full activity
45
half-life time: 66 min
45
-
gradual inactivation of the enzyme
45
-
isoform A, stable up to
50
-
purified enzyme, pH 5.8, half-live is 30 min
50
alpine bacterium
-
active below 50°C
50
-
the enzyme remains stable at temperatures up to 50°C
50
-
stable below, pH 6.0, 15 min
50
-
half-maximum inactivation after 1 h
50
-
pH 6, 1 h, about 50% loss of activity
50
-
gradual inactivation of the enzyme
50
-
the recombinant chitosanase is stable at temperatures up to 50°C above which the enzyme activity decreases rapidly
50
-
30 min, 20 mM acetate pH 5.0, 95% loss of activity
50
-
1 h, 25% residual activity for wild-type, 27% residual activity for mutant L74Q/V75I/G151D, 42% residual activity for mutant G151D/N222S
50
30 min, wild-type shows less than 37% of the initial activity, mutant enzyme G113C/D116C retains 62.0% of its initial activity, mutant enzyme A207C//L286C retains 81.0% of its initial activity
50
-
pH 6, 1 h, stable below
55
half-life: 10 min
55
-
significant loss in activity
60
-
purified enzyme, pH 5.8, half-live is 13 min
60
-
60 min, 40% loss of activity
60
-
30 min, pH 5.0, complete inactivation
60
-
pH 6.0, 15 min, 40% loss of activity
60
-
loss of 75% activity after 15 min
60
-
pH 5.5-9.0, stable below
60
the enzyme loses activity within 10 min at 60°C
60
30 min, 90% loss of activity
65
-
pH 6, 10 min, about 90% loss of activity
65
-
1 h, wild-type enzyme and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) retain 37% activity. Complete loss of activity of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35)
70
-
60 min, 70% loss of activity
70
-
15 min, 30% loss of activity
70
-
pH 6.0, 15 min, complete loss of activity
70
-
loss of 95% activity after 15 min
70
-
in presence of substrate remarkable stability at
70
Ficus sp.
-
30 min, enzyme retains almost all of its activity
70
-
the recombinant chitosanase shows hardly activity after 30 min
70
-
optimum for chitosanase acivity
75
0.5 h, 16% loss of activity. 1 h, 22% loss of activity
75
Ficus sp.
-
30 min, about 35% loss of activity
80
-
15 min, 55% loss of activity
80
-
60 min, 80% loss of activity
80
Ficus sp.
-
5 min, about 70% loss of activity
80
-
15 min, almost complete loss of activity
3.2.1.132 chitosanase
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