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Literature summary for 3.2.1.132 extracted from
- Purification and hydrolytic action of a chitosanase from Nocardia orientalis (1991), Biochim. Biophys. Acta, 1079, 65-72.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Amycolatopsis orientalis | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis orientalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amycolatopsis orientalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Amycolatopsis orientalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.4 | - |
after Sephadex G-75 chromatography | Amycolatopsis orientalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Amycolatopsis orientalis | ? | - |
? | |
| chitosan + H2O | enzyme is capable of cleaving between glucosamine and glucosamine or N-acetylglucosamine, but not cleaving between N-acetylglucosamine and glucosamine | Amycolatopsis orientalis | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | the smallest of the substrates is a tetramer of glucosamine | Amycolatopsis orientalis | additional information | mixture of dimer and trimer of glucosamine | ? | |
| chitosan + H2O | most susceptible substrates are: chitohexaose and chitoheptaose | Amycolatopsis orientalis | additional information | mixture of dimer and trimer of glucosamine | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Amycolatopsis orientalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
15 min, stable up to | Amycolatopsis orientalis |
| 80 | - |
15 min, 55% loss of activity | Amycolatopsis orientalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Amycolatopsis orientalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | more than 80% of activity maximum at pH 4.5 and 6.5 | Amycolatopsis orientalis |
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Release 2023.1 (January 2023)
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