KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 2.0% glycol chitosan | Bacillus sp. (in: Bacteria) | |
additional information | - |
additional information | Km: 3.3% soluble chitosan | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
41000 | - |
1 * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Bacillus sp. (in: Bacteria) | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
K-1 | - |
Bacillus sp. (in: Bacteria) | - |
No. 7-M | - |
Bacillus sp. (in: Bacteria) K-1 | - |
K-1 | - |
Bacillus sp. (in: Bacteria) No. 7-M | - |
No. 7-M | - |
Purification (Comment) | Organism |
---|---|
No. 7-M | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
319 | - |
after CM-Sephadex C-50 (II) chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | slow cleavage | Bacillus sp. (in: Bacteria) | ? | - |
? | |
chitosan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
chitosan + H2O | hydrolysis of: soluble chitosan | Bacillus sp. (in: Bacteria) | additional information | glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan | ? | |
chitosan + H2O | hydrolysis of: glycol chitosan | Bacillus sp. (in: Bacteria) | additional information | glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan | ? | |
additional information | no activity with chitin (i.e. 100% acetylated chitosan) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with glycol chitin | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with powdered chitosan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with cellulose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with methylcellulose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with colloidal chitin | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | no activity with chitin (i.e. 100% acetylated chitosan) | Bacillus sp. (in: Bacteria) K-1 | ? | - |
? | |
additional information | no activity with glycol chitin | Bacillus sp. (in: Bacteria) K-1 | ? | - |
? | |
additional information | no activity with powdered chitosan | Bacillus sp. (in: Bacteria) K-1 | ? | - |
? | |
additional information | no activity with cellulose | Bacillus sp. (in: Bacteria) K-1 | ? | - |
? | |
additional information | no activity with methylcellulose | Bacillus sp. (in: Bacteria) K-1 | ? | - |
? | |
additional information | no activity with chitin (i.e. 100% acetylated chitosan) | Bacillus sp. (in: Bacteria) No. 7-M | ? | - |
? | |
additional information | no activity with glycol chitin | Bacillus sp. (in: Bacteria) No. 7-M | ? | - |
? | |
additional information | no activity with powdered chitosan | Bacillus sp. (in: Bacteria) No. 7-M | ? | - |
? | |
additional information | no activity with cellulose | Bacillus sp. (in: Bacteria) No. 7-M | ? | - |
? | |
additional information | no activity with methylcellulose | Bacillus sp. (in: Bacteria) No. 7-M | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable below, pH 6.0, 15 min | Bacillus sp. (in: Bacteria) |
60 | - |
pH 6.0, 15 min, 40% loss of activity | Bacillus sp. (in: Bacteria) |
70 | - |
pH 6.0, 15 min, complete loss of activity | Bacillus sp. (in: Bacteria) |